Saccharopolyspora erythraea
Comparative genomics and transcriptional profiles of Saccharopolyspora erythraea NRRL 2338 and a classically improved erythromycin over-producing strain
... nomycete, Saccharopolyspora erythraea, has been widely studied as a model system for antibiotic production [13-16] and erythromycin and its semi-synthetic deriva- tives are widely used in the ...
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Dissecting and engineering of the TetR family regulator SACE_7301 for enhanced erythromycin production in Saccharopolyspora erythraea
... Saccharopolyspora erythraea, a Gram-positive industrial actinomycetes, produces a valuable macrolide antibiotic erythromycin A ...S. erythraea has been used for studying the biosynthesis and ...
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The dynamic regulation of nitrogen and phosphorus in the early phase of fermentation improves the erythromycin production by recombinant Saccharopolyspora erythraea strain
... Erythromycin is a kind of polyketide antibiotic produced by Saccharopolyspora erythraea through industrial fer- mentation. Recently, erythromycin has received much attention because its semi-synthetically ...
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Complete gene expression profiling of Saccharopolyspora erythraea using GeneChip DNA microarrays
... of Saccharopolyspora erythraea NRRL2338, a myc- elium-forming actinomycete and the producer of the clin- ically important macrolide antibiotic erythromycin ...S. erythraea, albeit originally ...
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A metabolic engineering approach to examine polyketide production by Saccharopolyspora erythraea
... S. erythraea physiology data and metabolic modelling predictions, over expression of a-ketoglutarate dehydrogenase was selected as a metabolic engineering ...S. erythraea strain with an over expressed ...
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Transcriptome-guided target identification of the TetR-like regulator SACE_5754 and engineered overproduction of erythromycin in Saccharopolyspora erythraea
... SACE_0388 , encoding a pyruvate, water diknase, catalyze pyruvate into phosphoenol-pyruvate, which may enter into TCA cycle. Methylmalonyl-CoA can either be filled or drained by methylmalonyl-CoA mutase- catalyzed ...
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Phosphate regulator PhoP directly and indirectly controls transcription of the erythromycin biosynthesis genes in Saccharopolyspora erythraea
... S. erythraea, the erythromycin production and the levels of ery mRNAs were regulated by nitrogen/phos- phate sources and their concentrations ...S. erythraea wild-type strain NRRL2338 was grown in ...
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Phenotypes and gene expression profiles of Saccharopolyspora erythraea rifampicin-resistant (rif) mutants affected in erythromycin production
... Results: Spontaneous rifampicin-resistant (rif) mutants were isolated from the parental strain NRRL2338 and two rif mutations mapping within rpoB, S444F and Q426R, were characterized. With respect to the parental strain, ...
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In silico reconstruction and experimental validation of Saccharopolyspora erythraea genome-scale metabolic model iZZ1342 that accounts for 1685 ORFs
... We have currently reconstructed and evaluated the genome-scale metabolic model (GEMs) of S. erythraea, called iZZ1342, which contains the latest gene annota- tion information, physiological parameters, and ...
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Process Synthesis for Antibiotic Recovery by Microfiltration from Saccharopolyspora erythraea Fermentations
... There are two rheological categories under which liquids and suspensions can be grouped, these are Newtonian and non-Newtonian fluids. For Newtonian fluids the viscosity, a measurement of the internal resistance to flow, ...
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The influence of agitation on morphology, rheology and erythromycin production in Saccharopolyspora erythraea culture
... Many types o f rheometer have been used to study the rheology of fermentation broths (See table 1.3). For this study a concentric cylinder was chosen against an impeller viscometer due to the difficulty in obtaining ...
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SACE_5599, a putative regulatory protein, is involved in morphological differentiation and erythromycin production in Saccharopolyspora erythraea
... S. erythraea wild type (WT) NRRL 23338 strain has been sequenced recently ...S. erythraea strains are continuing by genetic/metabolic en- gineering as well as classical methods, assisted by genomic and ...
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Effect of rapeseed oil on the nitrogen balance of laboratory and pilot-scale fermentation of Saccharopolyspora erythraea
... Too many variations in the media to make much of this, but in view of what follows the carbon limitation is important, as is the source of nitrogen. It can be seen that (Figure 5.1) the biomass of Medium 3 cultures give ...
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Integrated omics approaches provide strategies for rapid erythromycin yield increase in Saccharopolyspora erythraea
... In conclusion, the present study contributes a signifi- cant step forward in our understanding of how eryth- romycin biosynthesis is coordinated with the primary carbon metabolism and with the activities of other ...
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Optimization of medium using response surface methodology for erythromycin production by Saccharoplyspora Erythraea
... Peter, F., Patrick, C., Debra, J. and James, S. (1992). Identification of DEBS 1, DEBS 2 and DEBS 3, the multi enzyme polypeptide of the erythromycin-producing polyketide synthase from Saccharopolyspora ...
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Suitable extracellular oxidoreduction potential inhibit rex regulation and effect central carbon and energy metabolism in Saccharopolyspora spinosa
... in Saccharopolyspora erythraea , Streptomyces coelicolor , and Streptomyces avermitilis by using the BLASTP algorithm with significant sequence similarity (E value < 10 −40 ...of Saccharopolyspora ...
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Differential proteomic profiling reveals regulatory proteins and novel links between primary metabolism and spinosad production in Saccharopolyspora spinosa
... in S. coelicolor [37]. Although lysozyme M1 was not de- tected in our study, three lysozyme M1 precursor proteins were identified and one of these proteins (gi348170152, LM1 P) was highly abundant in S. spinosa and ...
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Complete genome sequence of butenyl-spinosyn-producing Saccharopolyspora strain ASAGF58
... The secondary metabolite-encoding gene clusters were predicted by submitting the whole-genome sequence in fasta format to the antiSMASH website (http://anti- smash.secondarymetabolites.org/) (Blin et al. 2013), and we ...
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Comparative Proteomic Analysis of saccharopolyspora spinosa SP06081 and PR2 strains reveals the differentially expressed proteins correlated with the increase of spinosad yield
... Many biological processes are intrinsically dynamic, incurring profound changes at both molecular and phy- siological levels. Systems analysis of such processes incorporating large-scale proteome profiling can be quite ...
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In Situ Activation and Heterologous Production of a Cryptic Lantibiotic from an African Plant Ant-Derived Saccharopolyspora Species
... Heterologous expression of the kyamicin BGC. Attempts to scale up cultures of Saccharopolyspora sp. KY21/pEVK4 to generate sufficient material for further study were not successful due to low titers and poor growth ...
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