Sample sections and confocal laser scanning microscopy (CLSM)
CONFOCAL LASER SCANNING MICROSCOPY TUTORIAL
... the confocal Pinhole restricts imaging to a thin Z ...illustration, laser light fills the back aperture of the objective lens and is focused to a Spot at a point ...
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The use of confocal laser scanning microscopy for the study of dentin infection
... 2.4 General sectioning, staining and CLSM procedures The samples of the in vitro and the extracted human teeth were sectioned using a 0.3mm Isomet saw under constant cooling using sterile distilled water. The roots were ...
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RESEARCH REPORT ON CONFOCAL LASER SCANNING MICROSCOPY STUDIES ON FURFURYLATED WOOD SAMPLES
... How is it possible to detect these changes in chemistry of a furfurylated wood product? To answer this question confocal laser scanning microscopy was used. The expectation was to detect ...
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Parametric Blind Deconvolution for Confocal Laser Scanning Microscopy (CLSM)-Proof of Concept
... fluorescence Confocal Laser Scanning Microscope (CLSM) images with parametric estimation of the acquisition sys- tem’s Point Spread Function ...
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Fluorescence excitation analysis by two-photon confocal laser scanning microscopy: a new method to identify fluorescent nanoparticles on histological tissue sections
... tissue sections of mouse aorta containing labeled iron nanoparticles stained with Texas Red and counterstained with SYTO13, to obtain visual information about the accumulation of these nanoparticles in the ...
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Confocal laser scanning microscopy analysis of S epidermidis biofilms exposed to farnesol, vancomycin and rifampicin
... antimicrobial therapy against S. epidermidis. However, its mechanism of action in S. epidermidis is not fully known. In this work we better elucidate the role of farnesol against S: epidermidis biofilms using ...
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Application of confocal laser scanning microscopy (CLSM) to visualize the effect of porous media wettability on unsaturated pore water configuration
... conventional microscopy arises from the capability to visualize the water phase constantly focused over the pore space, allowing the quantification of the area and number of wetted ...interest, sample ...
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Profile of bordered pit aspiration in Cryptomeria japonica using confocal laser scanning microscopy: pit aspiration and heartwood color
... make sections thin- ner than the diameter of the pit aperture and the prepara- tions involve embedding of specimens in ...consuming. Confocal laser scan- ning microscopy, which allows ...
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Development of useful recombinant promoter and its expression analysis in different plant cells using confocal laser scanning microscopy
... Figure 7. Expression of CaMV35S promoter and MSgt-FSgt promoter in different cell/tissue type of transgenic tobacco ( N. tabaccum cv Samsun NN) lines assayed in confocal laser scanning microscope ...
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LASER SCANNING CONFOCAL MICROSCOPY
... Abstract Laser scanning confocal microscopy has become an invaluable tool for a wide range of investigations in the biological and medical sciences for imaging thin optical sections in ...
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In vivo evaluation of DSAEK interface with scanning-laser confocal microscopy
... Similarly to others [17,18], we found no correlation between particle number and visual acuity. Small parti- cles located at the interface are a common finding fol- lowing DSAEK, and are also observed after LASIK surgery ...
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Cryo-laser scanning confocal microscopy of diffusible plant compounds
... temperature. As postulated [33], the extracellular water medium freezes first and provokes an outward water flux from the cells to osmotically equilibrate intracellular and extracellular media. Water in biomaterials is ...
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Correlative analysis of immunoreactivity in confocal laser-scanning microscopy and scanning electron microscopy with focused ion beam milling
... ADVANTAGE AND DISADVANTAGE OF THE IMMUNO-FIB-SEM METHOD The proposed immuno-FIB-SEM method was useful in the three- dimensional reconstruction of immunoreactive ultrastructures, and the contrast-inverted SEM images were ...
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Characterisation of solidification using combined confocal scanning laser microscopy with infrared thermography
... ACCEPTED MANUSCRIPT Figure 2: DSC traces during cooling at 0.5 °C/s for the pure iron and low carbon steel used in this study. Figure 3a shows the measured radiated heat taken from a pixel from the surface of the molten ...
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Iris ultrastructure in patients with synechiae as revealed by in vivo laser scanning confocal microscopy
... We found that the support structure of the iris branches gradually from coarse to fine. Based on the size of the support structure, the stent surface of iris can be divided into three classes of structures: tree trunk-, ...
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Studies of the microstructure of polymer modified bitumen emulsions using confocal laser scanning microscopy
... Polymer-modified bitumen emulsions present a safer and more environmentally friendly binder for enhancing the properties of roads. Cationic bitumen emulsion binders containing polymer latex were investigated using ...
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The neuromuscular system of Pycnophyes kielensis (Kinorhyncha: Allomalorhagida) investigated by confocal laser scanning microscopy
... 5 Fig. 2 Musculature of Pycnophyes kielensis; anterior with introvert inverted. Micrographs obtained with a confocal laser scanning microscope of two kinorhynchs with introvert slightly everted. The ...
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Histometric data obtained by in vivo confocal laser scanning microscopy in patients with systemic sclerosis
... histological sections by loss of melanin into the dermis, which would probably not influence this parameter, there seems to be an activation of melanocytes leading to a higher amount of melanosomes in the basal ...
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Confocal laser scanning microscopy to estimate nanoparticles’ human skin penetration in vitro
... corneum, confocal laser scanning micros- copy, tape stripping Background Nanotechnology, a rapidly emerging field, provides new techniques and ...
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Elucidating the pulsed-dye laser treatment of sebaceous hyperplasia in vivo with real-time confocal scanning laser microscopy
... CM operates by tightly focusing a laser beam on a specific point in the skin, detecting only the light reflected from the focal point through a pinhole- sized spatial filter. 10,11 The beam can then be scanned ...
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