Sandwich enzyme-linked immunosorbent
A reverse type sandwich enzyme linked immunosorbent assay for detecting antibodies to Borna disease virus
... reverse-type sandwich enzyme-linked immunosorbent assay (RS-ELISA) for detecting specific antibodies to ...an enzyme substrate were used to measure the captured biotinylated ...
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Development and Application of a Double-Antigen Sandwich Enzyme-Linked Immunosorbent Assay for Detection of Antibodies to Porcine Circovirus 2
... double-antigen sandwich enzyme-linked immunosorbent assay (ELISA) is described for detection of porcine circovirus 2 (PCV2) antibodies using the well-characterized recombinant PCV2 capsid ...
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Development of a Quantitative Sandwich Enzyme Linked Immunosorbent Assay for Detecting the MPT64 Antigen of Mycobacterium tuberculosis
... a sandwich enzyme- linked immunosorbent assay (ELISA) against the MPT64 protein of ...A sandwich ELISA was established using recombinant MPT64 protein and anti-MPT64 monoclonal ...The ...
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Development of a sandwich enzyme-linked immunosorbent assay for dTMP-GH fusion protein by rational immunogen selection
... sensitive sandwich enzyme- linked immunosorbent assay (ELISA) with two antibodies (one polyclonal and one HRP-conjugated monoclonal) to quantify ...
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Development and application of triple antibody sandwich enzyme linked immunosorbent assays for begomovirus detection using monoclonal antibodies against Tomato yellow leaf curl Thailand virus
... Development and application of triple antibody sandwich enzyme linked immunosorbent assays for begomovirus detection using monoclonal antibodies against Tomato yellow leaf curl Thailand virus RESEARCH[.] ...
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Diagnostic efficacy of monoclonal antibody based sandwich enzyme linked immunosorbent assay (ELISA) for detection of Fasciola gigantica excretory/secretory antigens in both serum and stool
... In the present study, our MoAbs were able to detect F. gigantica antigens level ≥ 3 ng/ml in sandwich ELISA. This could be attributed to the use of a pair of MoAbs, one as antigen capturing antibody (12B/11D/3F) ...
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Indirect Sandwich Enzyme Linked Immunosorbent Assay for Rapid Detection of Haemophilus influenzae Type b Infection
... A total of 18 clinical specimens spinal fluid, 11; urine, 5; serum, 2 from 11 patients with culture-proved HIb meningitis were obtained for testing by the indirect sandwich ELISA assay T[r] ...
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Indirect sandwich enzyme linked immunosorbent assay for rapid detection of Streptococcus pneumoniae type 3 antigen
... indirect sandwich ELISA assay that is capable of detecting low levels of the polysaccharide antigen of Streptococcus pneumoniae type 3 in clinical samples of human spinal fluid, urine, s[r] ...
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Double antibody sandwich enzyme linked immunosorbent assay for rapid detection of toxin producing Corynebacterium diphtheriae
... The plates were then reacted with mouse monoclonal diphtheria antitoxin 17.6, 0.1 ,ug/ml A or various concentrations B, followed by incubation with labeled anti-mouse IgG diluted 1: 1,00[r] ...
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Monoclonal antibody based sandwich enzyme linked immunosorbent assay for detection of Bordetella pertussis filamentous hemagglutinin
... Monoclonal antibody 16-2-D9 and biotinylated monoclonal antibody 15-2-A5 A and polyclonal goat anti-FHA serum and monoclonal antibody 16-1-E3 B were used as capture and second antibodies[r] ...
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Enzyme linked immunosorbent assay that uses labeled antigen for detection of immunoglobulin M and A antibodies in toxoplasmosis: comparison with indirect immunofluorescence and double sandwich enzyme linked immunosorbent assay
... Of 80 single sera with high antibody titer to toxoplasma in indirect immunofluorescence and complement fixation, 40 were positive in the direct ELISA for ToxIgM, 36 were positive in the [r] ...
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Rapid and Sensitive Detection of Immunoglobulin M (IgM) and IgG Antibodies against Canine Distemper Virus by a New Recombinant Nucleocapsid Protein Based Enzyme Linked Immunosorbent Assay
... capture-sandwich enzyme-linked immunosorbent assay (ELISA) that uses recombinant bacu- lovirus-expressed nucleocapsid (N) protein of a recent CDV wild-type isolate (2544/Han95) for the ...
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Serological differentiation between cystic and alveolar echinococcosis by use of recombinant larval antigens
... A sandwich enzyme-linked immunosorbent assay with monoclonal antibodies against EM10 and EG55 as capture reagents for the recombinant antigens was established and was evaluated with 74 s[r] ...
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Development and application of enzyme linked immunosorbent assay for specific detection of Salmonella enteritidis infections in chickens based on antibodies to SEF14 fimbrial antigen
... double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) was developed to detect anti- bodies to the SEF14 fimbrial antigen (SEF14-DAS ELISA) and was evaluated for its use in the ...
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Monoclonal Antibodies Specific for Immunorecessive Epitopes of Glucuronoxylomannan, the Major Capsular Polysaccharide of Cryptococcus neoformans, Reduce Serotype Bias in an Immunoassay for Cryptococcal Antigen
... Immunoassay for detection of glucuronoxylomannan (GXM), the major capsular polysaccharide of Crypto- coccus neoformans, is an important tool for diagnosis of cryptococcosis. However, immunoassays that are based solely or ...
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Comparison of enzyme linked immunosorbent assay systems using rift valley fever virus nucleocapsid protein and inactivated virus as antigens
... an enzyme-labeled secondary ...IgG sandwich ELISA and IgM capture ELISA systems for humans have not been ...IgG sandwich ELISA is more sensitive than IgG indirect ELISA ...
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Detection with monoclonal antibody of Salmonella typhi antigen 9 in specimens from patients
... Antibodies produced to antigen 9 of group D salmonella were used in double- and triple-sandwich antibody enzyme-linked immunosorbent assays ELISAs for detecting antigen 9 in urine and pl[r] ...
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Comparison of Two Gamma Interferon Release Assays and Tuberculin Skin Testing for Tuberculosis Screening in a Cohort of Patients with Rheumatic Diseases Starting Anti-Tumor Necrosis Factor Therapy
... by enzyme-linked immunosorbent assay (ELISA) (QuantiFERON-TB Gold [QFT-G] and QuantiFERON-TB Gold In Tube [QFT-GIT] assays; Cellestis Limited, Carnegie, Victoria, Australia) or by ...
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Development and evaluation of enzyme-linked immunosorbent assay of nucleic acid sequence-based amplification for diagnosis of invasive aspergillosis
... our experiment. Further more, a potential risk of con- tamination with such non-target microbes nucleic acids could be ignored because only the target nucleic acids with T7 promoter can be amplified. Thus, the hybridi- ...
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Enzyme-linked immunosorbent assay of nicotine metabolites
... Fig. 1 Urine from 93 kindergarten children analyzed by enzyme- linked immunosorbent assay (ELISA) and by chromatography–mass spectrometry (GC–MS). a Free and total cotinine levels determined by GC–MS ...
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