Semliki Forest virus (SFV)
Protection against lethal simian immunodeficiency virus SIVsmmPBj14 disease by a recombinant Semliki Forest virus gp160 vaccine and by a gp120 subunit vaccine.
... immunodeficiency virus (SIV) model as a rapid and rigorous challenge to evaluate the efficacy of two SIV Env vaccine ...recombinant Semliki Forest virus expressing the SIV-PBj14 Env gp160 ...
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Nairovirus RNA Sequences Expressed by a Semliki Forest Virus Replicon Induce RNA Interference in Tick Cells
... to virus infection and has been illustrated in several viral infection ...dengue virus, La Crosse virus, or Rift Valley fever virus (7, 14, ...homologous virus (8, ...the Semliki ...
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Furin Processing and Proteolytic Activation of Semliki Forest Virus
... alphavirus Semliki Forest virus (SFV) infects cells via low-pH-dependent membrane fusion in endocytic organelles (reviewed in references 24 and ...The SFV membrane con- tains ...
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The 6-kilodalton membrane protein of Semliki Forest virus is involved in the budding process.
... as Semliki Forest virus (SFV) and Sindbis virus (SIN) consist of an RNA-containing nucleocapsid envel- oped by a host-derived membrane in which are inserted two virus-encoded ...
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Role of Conserved Histidine Residues in the Low-pH Dependence of the Semliki Forest Virus Fusion Protein
... trigger virus-membrane fusion. For alphaviruses such as Semliki Forest virus (SFV), acidic pH initiates a series of conformational changes in the heterodimeric virus envelope ...
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Effect of intranasal administration of Semliki Forest virus recombinant particles expressing interferon ? on the progression of experimental autoimmune encephalomyelitis
... of Semliki Forest virus (SFV) recombinant particles expressing interferon-ß [IFN-ß, a partially effective treatment for multiple sclerosis (MS)] on the progression of experimental auto- immune ...
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Biogenesis of the Semliki Forest Virus RNA Replication Complex
... as Semliki Forest virus (SFV) and Sindbis virus, has been studied intensively over more than 30 ...in SFV-infected chicken embryo fibroblasts were defined as type I cytoplasmic ...
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Identification of distinct antigenic determinants on Semliki Forest virus by using monoclonal antibodies with different antiviral activities.
... 575 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Fifteen monoclonal antibodies MAs directed against either the El or E2 glycoprotein of Semliki Forest virus SFV were[r] ...
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Expression of Semliki Forest virus nsP1-specific methyltransferase in insect cells and in Escherichia coli.
... * ogy, 7418 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest We have expressed the Semliki Forest virus SFV-specific nonstructural protein nsPl both in insect cells and i[r] ...
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Deletion Analysis of a Defective Interfering Semliki Forest Virus RNA Genome Defines a Region in the nsP2 Sequence That Is Required for Efficient Packaging of the Genome into Virus Particles
... of Semliki Forest virus (SFV) defective interfering (DI) RNA 19 (DI-19) is coterminal with the infectious genome and contains two major ...DI SFV RNAs, DI-19 contains three regions that ...
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Biochemical Consequences of a Mutation That Controls the Cholesterol Dependence of Semliki Forest Virus Fusion
... the virus during bud- ...wt SFV and srf-3 and determined that both virus preparations had comparable ratios of pyrene excimer to monomer fluorescence, indicating com- parable densities of pyrene ...
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An Epitope of the Semliki Forest Virus Fusion Protein Exposed during Virus-Membrane Fusion
... Sindbis virus E1 protein. Sindbis virus is another well-characterized member of the alphaviruses (40) that has been shown to enter cells by a membrane fusion reaction that is dependent on low pH (14) and ...
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Studies on defective interfering Semliki Forest virus
... DI SFV particles were required to protect the majority of mice against 10 LD^ q (6000 pfu) S ...DI SFV protects mice by intrinsic interference rather than by the host's immune ...S virus from the ...
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Cell-to-Cell Spread of the RNA Interference Response Suppresses Semliki Forest Virus (SFV) Infection of Mosquito Cell Cultures and Cannot Be Antagonized by SFV
... the SFV replicons SFV(nseGFP/H)1 (control replicon [CTRL]) (A and B), SFV(nseGFP/ H)1-NS1 (A), or SFV(nseGFP/H)1-p19 (B) and then incubated for 24 h to allow replicon-derived dsRNA/siRNA ...
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Reversible Acid-Induced Inactivation of the Membrane Fusion Protein of Semliki Forest Virus
... of SFV E1 with target membranes ...intact virus, exposure of the E1 fusion peptide was dependent on low pH, consistent with the notion that the fusion peptide is shielded in the native virus by E2 ...
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Semliki Forest Virus in HEp-2 Cell Cultures
... West Nile virus growvacuolar membranes SFV particles were ing in HEp-2 cell cultures formed in granular foci in KB cells infected with Venezuelan equine in the cytoplasm and filled chann[r] ...
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Studies with temperature-sensitive mutants of Semliki Forest virus
... RNA mutants were used to test if inhibition of cellular RA synthesis could occur in the absence of viral RNA synthesise Two major proteins are found in the virions of Semliki Forest viru[r] ...
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Role of ribosomes in Semliki Forest virus nucleocapsid uncoating.
... Virus was bound at 4°C and internalized at 37°C in the presence of 200 ,uM puromycin Sigma to inhibit protein synthesis and thus avoid confusion between incoming and newly synthesized vi[r] ...
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Karyophilic properties of Semliki Forest virus nucleocapsid protein.
... Low concentrations of highly purified native C protein 103 to 104 molecules per cell transferred into various target cells induce the synthesis of specific host cellular proteins 10.. Hi[r] ...
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Phenotypic changes in Langerhans' cells after infection with arboviruses: a role in the immune response to epidermally acquired viral infection?
... Nile virus (WNV), and an alphavirus, Semliki Forest virus (SFV), being ...local, virus-directed, and/or host- directed responses and may modulate T-cell activation during viral ...
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