Separation of proteins using hydrophobic interaction chromatography
Separation of biological proteins by liquid chromatography
... thousands proteins in living organisms and a set of them is called as proteome and unlike genome, the prote- ome differs from cell to cell ( Garcia et ...The separation and identification of proteomics is a ...
15
Separation of Cancer Proteins using Polyacrylamide Gel Electrophoresis
... the proteins present in serum has detected. The isolation of proteins involves the appropriate methods ...of proteins by electrophoresis following fractionation of human blood ...The proteins ...
8
Separation and identification of carp pituitary proteins and glycoproteins
... Affinity chromatography on Con ...and separation was done according to Hulová et ...of proteins was monitored by measuring the absorbance of the eluate at 280 ...
9
Chromatographic Separation and Antigenic Analysis of Proteins of the Oncornaviruses
... phy in GuHCI of MC29 labeled with a mixture of radioactive amino acids permitted a calculation of the relative numbers of protein molecules of each type present in the virion. The per ce[r] ...
8
Hydrophobic Interaction Chromatography
... for Hydrophobic Interaction Chromatography Hydrophobic interaction chromatography (HIC) is a powerful tool for the process purification of ...accessible hydrophobic ...
11
GE Healthcare. Hydrophobic Interaction and Reversed Phase Chromatography. Principles and Methods
... liquid chromatography (MDLC) The Cipp strategy is well suited for purification of a target protein or ...However, chromatography techniques are also successfully combined to achieve identification and ...
168
Purification of Mycobacterium bovis BCG Tokyo antigens by chromatofocusing, lectin-affinity chromatography, and hydrophobic interaction chromatography.
... lectin-affinity chromatography, and hydrophobic interaction chroma- tography resulted in a simple purification of protein antigens of Mycobacterium bovis BCG Tokyo culture ...chromatographic ...
6
Separation of Amino Acids by Paper Chromatography
... The 20 different amino acids that make up our proteins, and those of most other living things, differ in the identity of the side chain R. In glycine, the simplest amino acid, R is a hydrogen atom. Eight amino ...
5
Separation of RNA & DNA by Gel Filtration Chromatography
... of proteins, polysaccharides and nucleic ...matrix, chromatography column and the elution ...the chromatography and flows through the matrix and out of the ...
30
Optimization of Lactoperoxidase and Lactoferrin Separation on an Ion-Exchange Chromatography Step
... 3.2 Experimental methods In all experimental setups the column was at first equilibrated with running buffer for 5 column volumes (CV). This step was followed by an automated sample load of 2 mL protein mixture. Then the ...
23
Understanding and manipulating the separation in hydrophilic interaction liquid chromatography
... ionic interaction (measured from alpha benzyltrimethylammonium ...uncharged using the mobile phase conditions in the ...ionic interaction, as ...
58
Native hydrophobic interaction chromatography hyphenated to multi-angle light scattering
... produce NP with high yield and product purity, using the CASPON technology for fusion-protein 95.. production 28,29.[r] ...
36
Separation of organophosphorus pesticides using micellar electrokinetic chromatography
... three hydrophobic OPPs and SRMM for three hydrophilic OPPs were expected to be useful either by direct injecting the sample upon filtration or with prior suitable offline pre- concentration ...
52
Separation of organophosphorus pesticides using micellar electrokinetic chromatography
... that separation of OPPs was incomplete regardless of changing all sort of possible factors such as buffer types, buffer and surfactant concentrations, addition of organic modifier, methanol and pH of the running ...
9
Investigation of host–pathogen interaction between Burkholderia pseudomallei and autophagy-related protein LC3 using hydrophobic chromatography-based technique
... bacterial proteins of both wild type and rpoS mutant, which were similar to MS signals in negative control column, had been ruled out in this ...LC3-interacting proteins among wild type and rpoS mutant. ...
13
Hydrophilic interaction liquid chromatography: An update
... biopharmaceuticals using gradients of 65-80 % ACN and ...better separation of insulin and insulin glulisine by HILIC, but superior separation of insulin and insulin lispro by ...
22
Analysis of Lidocaine Interactions with Serum Proteins Using High-Performance Affinity Chromatography
... affinity chromatography was used to study binding by the drug lidocaine to human serum albumin (HSA) and α 1 –acid glycoprotein ...serum proteins and is transported in the ...serum proteins and ...
12
Characterization Of Drug Interactions With Serum Proteins by Using High-Performance Affinity Chromatography
... chromatographic supports for use in drug binding studies. It was described how HPAC has already been employed with many drugs and with binding agents such as HSA, AGP and lipoproteins. Methods that have been used in ...
37
Application of binary packing for starch separation by hydrodynamic chromatography
... In the case of a binary packing bed, shaped micro-particles display a different behaviour than the one exhibited by spherical particles of the same scale. This might be explained by the interaction between shaped ...
5
Application of binary packing for starch separation by hydrodynamic chromatography
... In the case of a binary packing bed, shaped micro-particles display a different behaviour than the one exhibited by spherical particles of the same scale. This might be explained by the interaction between shaped ...
5