Eyoh, G. D., Etukapan, E., N. B. Idiong,Ubak, E, Ekpo, J. S. & Sam, I.M. (2016). Hematological Profile And SerumBiochemistry Of West African ` Dwarf (WAD) Goats In Obio Akpa, Akwa Ibom State, Nigeria. 21st Animal Science Association of NigeriaASAN-NIAS Annual Conference, University of Port Harcourt. 18th - 22nd September, 948 – 951.
creatinine content of serum. Birds onT1 were not significantly different (P > 0.05) from birds on T2, but T1 and T2 birds were significantly difference (P < 0.05) from T2 and T3 birds. 20mg clomiphene treated Chicken were not significantly different (P > 0.05) from T4. However, the lowest value (70.00mmol/L) for creatinine was observed on the T4 while the highest value (135.55mmol/L) was recorded in T1. A major source of creatinine in the blood is from the muscle when wasting occurs and creatinine phosphate catabolized, (Bale et al., 2000). There were significant differences (P < 0.05) among the treatment groups in glucose constituent of serumbiochemistry. Birds in T1 recorded the highest value in serum glucose (160.55mmg/100ml) and this differed significantly (P< 0.05) from birds on T2, T3 and T4. Treatment2(T2 clomiphene citrate treated birds were also significantly different from 20mg clomiphene treated cocks, 20mg clomiphene treated birds were also significantly different (P < 0.05) from 30mg clomiphene treated birds their glucose serum level. Bird on T3 recorded the lowest value in serum glucose (75.75 g/100ml). There was significant difference (P < 0.05) between the control and the other three treatment groups in serum total protein. Cocks on 10mg clomiphene treatment, cocks on 20mg clomiphene treatment and birds on 30mg clomiphene treatment were not significantly different (P > 0.05) in serum total protein. Birds
Mean values for hemoglobin (g/dl) differed significantly (P<0.05) between different genotype of poultry. In turkey, highest hemoglobin was recorded in local (15.09 g/dl), followed by Exotic (14.31g/dl and least in crossbred (12.42 g/dl). The mean values for PCV (%) were significantly (P<0.05) different with highest in exotic (36.00) followed by crossbred (33.01) and least in local turkey (32.62). Mean values for MCV (fl) were significantly (P<0.05) highest in crossbred (160.23) followed by exotic (156.24) and least in local (146.71). The mean values for MCH (pg) were significantly (P<0.05) highest in exotic (73.83), followed by local (69.33) and least in crossbred (59.47). The mean value of MCHC (g/dl) were significantly (P<0.01) highest in exotic (48.13) followed by local (47.05) crossbred (41.51). There was significant (P<0.05) differences between the genotypes in the total protein, albumin, globulin, cholesterol, urea, sodium(Na+) , potassium (K+), calcium and phosphorus of the genetic groups. The crossbred and exotic genotype however, had the highest significant albumin (1.43 and 1.41 ml) and lower globulin values (3.87 ml) were observed in the crossbred compared to the other turkey genotypes. Variation in these results may be due to difference in breeds. It is concluded that significant differences in haematological value and erythrocyte indices and serumbiochemistry among different genotype of turkey could be considered as a references values and this may serve as a guide to assess the state of health in the monitored birds.
Clarias species is a widely distributed fish which constitutes one of the major fisheries in Asia and Africa. Some records have shown that Clarias contributes about 14% of over 6,000 tonnes of annual fish production from all fisheries sectors . The common species found in Nigeria are Clarias gariepinus, Clarias angullaris and Clarias lazare. The Clarias gariepinus is a prominent cultured species because of its hardiness and fast growth rate. Several investigations have been carried out on various toxicants with Clarias sp. . The objective of this study was to evaluate the effects of Vestalin Herbicide on the heamatological parameters and blood serumbiochemistry of Clarias gariepinus juveniles.
Quinoa is a pseudo-cereal with the variety of vitamins, minerals contents, important proteins, essential unsaturated fatty acids and carbohydrates with less glycemic index. The current study was designed to evaluate the effects of quinoa seed meal on serumbiochemistry of broiler birds. Seventy (one day old) chicks were raised and divided into groups (n=6) with ten birds (n=10) in each group. The total duration of the experiment was 42 days and all the birds were fed with commercially available feed from market (Starter)from day 1 to day 14. From day 15 to day 42 all the birds in group 1 were fed with the dietcontaining50g/kg quinoa seeds meal. Group 2 birds were fed with the dietcontaining100g/kg quinoa seed meal. Group 3 birds were fed with 200g/kg quinoa seeds meal as a protein source. From day 21 to day 42 all birds from group 4 were fed with 50g/kg quinoa seed meal. Group 5 chicks were fed with the dietcontaining100g/kg quinoa seeds meal and group 6 chicks were fed with diet contain quinoa seed meal (200g/kg) as a protein source. Control birds were fed with 100% commercially available feed. In results, significant effects of quinoa meal in the trial. 2 and trial3 improved kidney functions (ALT & AST) and liver functions (Urea &Creatinine). The quinoa phytochemicals indicate major role as protein meal in the poultry feed through a multiplicity of effects.
assess the effect of sun dried (raw) (SD), dehulled (D) and boiled k idney beans (BKB)on the haematological and serumbiochemistry of broiler birds. One hundred and twenty unsexed broiler birds were used for the study. The birds were randomly assigned to four dietary treatments replicated three (3) times with twelve (12) birds per replicate in a completely randomized design (CRD). The treatment groups were control diet (CD), sun dried k idney bean (SDKB), dehulled k idney bean (DKB) and boiled k idney bean (BKB), designated as T1, T2, T3 and T4, respectively. The feeding trial lasted for four week s.The proximate composition of the k idney beans used was also determined. The proximate composition obtained showed that k idney beans is a good protein source for birds (crude protein content of 20.98%). At the end of the feeding trial, blood samples were collected and the haematological and serum biochemical parameters of the birds were determined using standard methods. Generally, the diets used exhibited significant effects on both the haematological and serum biochemical parameters of the birds studied. Diet without k idney bean (Control) and diet containing sun dried k idney bean gave the best result, though all the haematological and biochemical values obtained in the studyfall within the referral range indicating that processing method had no negative effect on the haematological and serum biochemical parameters of the birds. Based on the findings in this study, it was recommended that k idney bean is a good source of plant protein in animal diet and does not necessarily need to be processed prior to its incorporation in animal feed.
ABSTRACT: In this study the effects of monosodium l-glutamate (MSG) administration on the reproductive param- eters and serumbiochemistry of male rabbits were investigated. Sixteen mature male New Zealand mixed-breed of rabbits (Oryctolagus species) weighing 1.1–1.65 kg were used for this study. They were randomised into four groups of four rabbits each. Group A which served as the untreated control, received only distilled water while Groups B, C and D which were the treated groups, received 0.25 g/kg, 0.5 g/kg and 1 g/kg body weight of MSG from a 40% MSG stock solution. The MSG was administered to the rabbits by oral gavage every forty-eight hours for a period of eight weeks. Serum levels of luteinising hormone (LH), testosterone, total cholesterol and total protein, and activi- ties of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were evaluated on Days 14, 28 and 56 of MSG administration. Results showed that when compared to the untreated group (Group A), on Day 56 of MSG administration the mean serum levels of both LH and testosterone were significantly (P < 0.05) lower in Group D that received 1 g/kg body weight MSG, while the serum cholesterol levels of Group C rabbits that received 0.5 g/kg body weight MSG was significantly (P < 0.05) lower on Day 28 of MSG administration. The mean serum ALT activity of Groups B and C rabbits were significantly (P < 0.05) lower on Day 56 of MSG administration, while the mean serum AST activity of the Group D rabbits was significantly (P < 0.05) higher on Day 14 of MSG administration. There were no significant (P > 0.05) variations in the mean serum total protein between the groups. Testicular histomorphology revealed that MSG administration did not affect the testes of the rabbits as there was no obvious testicular histopa- thology. It was concluded that administration of MSG to male rabbits significantly lowered serum LH, testosterone and cholesterol levels and serum ALT activity without affecting testicular histomorphology.
In our recent studies, we found that high dietary F induced oxidative stress in the cecal tonsil and the intestin- al mucosa of broiler chickens  . However, limited studies have focused on the effects of high dietary F on serumbiochemistry and oxidative damage parameters in broiler chickens. Chen et al.  have reported that high dietary F decreases the superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activities and in- creases the malondialdehyde (MDA) content in the serum of broiler chickens. In addition, Liu et al.  have reported that F decreases the activities of SOD, catalase (CAT), GSH-Px and increases the MDA content in the serum of chicks, but the serum biochemical indexes, and the ability to inhibit hydroxyl radical and glutathione (GSH) content in the serum have not been reported in their experiments. Abdelhamid and Dorra  have stu- died that feed-borne F intoxication decreases the content of total protein (TP), albumin (ALB) and hepatic en- zymatic activity in broiler chicks.
Abstract. Two hundred and twenty five (225) unsexed Marshal Broiler chicks were used to investigate the carcass yield, organoleptic and serumbiochemistry of broilers fed activated charcoal. The broiler chicks were randomly allotted to five dietary treatments, supplemented with activated charcoal at 0, 0.5, 1, 1.5 and 2% and designated as T1, T2, T3, T4 and T5, respectively. Each treatment was grouped in three replicate with 15 birds per replicate. At the end of 8 weeks experimental period, a total of 15 broiler chickens were used for both carcass and blood analysis with three birds per treatment. Data was collected on carcass characteristics, sensory evaluation, and blood serum cholesterol. There were significant (P < 0.05) differences in the values of dressed weight, eviscerated weight, gizzard, abdominal fat, heart and spleen, while other carcass parameters were not significantly (P > 0.05) different. The result of the sensory evaluation were significant in all the parameters measured, while the serum biochemical analysis showed that SGOT (serum glutamine oxaloacetic transminase, SGPT (serum glutamine phosphates transminase), albumin, cholesterol and triglycerides were significantly (P < 0.05) different except alkaline phosphates and SGPT that were not significantly different. However, activated charcoal would serve as a good source of growth promoter for broiler birds to improve feed efficiency and their health. It is therefore concluded that at 0.5% activated charcoal supplementation increased relative organ weights, reduced cholesterol level of the broiler meat as a result or efficient mineral intake and nutrient utilization due to the absorptive effect of the activated charcoal.
Abstract: Blood samples were obtained from 155 North American river otters (Lontra canadensis; 94 adult males, 38 adult females, 10 juvenile males, and 13 juvenile females) to establish baseline hematology and from 50 adult river otters (40 males and 10 females) for baseline serumbiochemistry parameters for the species. The otters were livetrapped from eastern North Carolina (USA) during a 4-yr period. Data for 14 routine hematologic parameters and 22 serum chemistry assays showed significant differences in total leukocyte count and absolute neutrophil and monocyte numbers for adults versus juveniles, red blood cell counts and hemoglobin between adult and juvenile males, and calcium and alkaline phosphatase values for adult males between years of the study and an increase in leukocyte counts and absolute neutrophils with increased degree of trap injury sustained.
A 42 days experiment was carried out to evaluate the effects of dried Centella asiatica leaf meal (CSP) as an herbal feed additive on the growth performance, haematology and serumbiochemistry of broiler chicken. A total of 200 Ross 308 day old broiler chicks of mixed sex was divided into four (4) treatment groups of fifty (50) birds, each group was further divided into five replicates each containing ten (10) birds. Group A was fed basal diet + 0% CPS (control), group B,C and D were fed basal diet + 2%, 4% and 6% CPS, respectively.The basal diet was formulated to meet the nutritional requirements of birds according to NRC (1994). Feed and water were provided unrestricted and a completely randomized design and birds were vaccinated according to the prevailing disease in the environment. The obtained results showed that there were significant (p<0.05) differences among all treatments in the values of final live weight, body weight gain and feed conversion ratio. Diet containing 6% CPS had the highest weight gain of 2072.9grams, while broilers fed 0% CPS had the lowest weight gain of 1534.7 grams. Supplementation of CPS did not affect (p>0.05) the daily feed intake and mortality rate. All the hematological (PCV, Hb, RBC, MCV, MCH, MCHC, WBC and its differentials) and serum biochemical parameters (Albumin, globulin, total protein, SGPT and SGOT) were not significantly (p>0.05) different among the treatments.
A 42 days experiment was carried out to evaluate the effects of dried Centella asiatica leaf meal (CSP) as an herbal feed additive on the growth performance, haematology and serumbiochemistry of broiler chicken. A total of 200 Ross 308 day old broiler chicks of mixed sex was divided into four (4) treatment groups of fifty (50) birds, each group was further divided into five replicates each of ten (10) birds. Group A was fed basal diet + 0% CPS (control), group B,C and D were fed basal diet + 2%, 4% and 6% CPS respectively. The basal diet was formulated to meet the nutritional requirements of birds according to NRC (1994). Feed and water was provided unrestricted and a completely ran- domized design and birds were vaccinated according to the pre- vailing disease in the environment. The results obtained showed that there were significant (p<0.05) differences among all treat- ments in the values of final live weight, body weight gain and feed conversion ratio. Diet containing 6% CPS had the highest weight gain of 2072.9 grams, while broilers fed 0% CPS had the lowest weight gain of 1534.7 grams. Supplementation of CPS did not affect (p>0.05) the daily feed intake and mortality rate. All the hematological (PCV, Hb, RBC, MCV, MCH, MCHC, WBC and its differentials) and serum biochemical parameters (Albumin, globu- lin, total protein, SGPT and SGOT) were not significantly (p>0.05) different among the treatments.
The study was carried out at the grasscutter unit of the Department of Animal Science, University of Nigeria, Nsukka. Nsukka lies in the derived savannah region, and is located on Longitude 6°25'N and Latitude 7°24'E (Ofomata, 1975) at an altitude of 430m above sea level (Breinholt et al., 1981). The climate is a typical humid tropical setting with a RH range of 56.01- 103.83%. Average diurnal minimum temperature ranges from 22°C – 24.7°C while the average maximum temperature ranges between 33°C – 37°C (Okonkwo & Akubuo, 2007). Annual rainfall ranges from 1680mm – 1700mm (Breinholt et al., 1981). This study was carried out to determine the effects of tropical herbs on the serumbiochemistry and haematological characteristics of weaner grasscutters. In particular, the study determined the effect of Ocimum gratissimum, Moringa oleifera, and Gongronema latifolia as supplementary sources of vitamins and minerals, on the haematological and serum biochemical parameters of weaner grasscutters. A total of 20 weaner grasscutters comprising of four males and sixteen females at the age of eight weeks were used for the study. The experimental animals were allowed two weeks of stabilization period and feeding trials lasted for twelve weeks. Five grasscutters were randomly allocated to each treatment. The floor of the pens were cleaned daily, feed and water were provided ad libitum. Leaves were air dried under shade for ten days, all leaves were threshed carefully to separate leaves from twigs before blending. Twenty percent (20%) of M. oleifera, (20%) G. latifolia (20%) O. gratissimum leaf meals were added to each supplemented diet. The experimental animals were allowed two weeks stabilization period and feeding trials lasted for twelve (12) weeks.
To study the working mechanisms for non-starch polysaccha- rides’ to improve the growth performance of broiler chickens, a 35-day feeding trial was conducted. Two dietary treatments were included: 1) wheat diet (the control); 2) wheat+xylanase diet .There were 5 groups with 3 replicates ,each replicates having 8 birds each for each treatment and the experimental diets were given to birds from hatch. Group A was control and group B was treated with Xylanase concentration of 250IU/Kg feed, Group C was treated with Xylanase concentration of 500IU/Kg feed, Group D was treated with Xylanase concentration of 750IU/Kg feed and Group E was treated with Xylanase concentration of 1000IU/Kg feed.FCR and body weight were measured weekly. The vaccination was done according to schedule. Fresh Water provided at free labitum. The shed was properly maintained with respect to temperature and humidity. The Fumagination was properly and thoroughly done before the start of experiment. The xylanase supplement increased (p<0.05) body weight gain (BWG) and improved feed conversion ratio (FCR) at the end of the experiment but non-significant results were observed on se- rum biochemistry (p>0.05) by xylanase. (Key Words: Xylanase, weight gain, Serumbiochemistry.
Summarized haematological data on small blood count revealed that these four blood parameters are fairly similar despite of experiment or animal material. All haematological analyses were done by the same laboratory which probably declined variability of results. It looks that small blood count can be reliably used to screen gen- eral welfare status of farmed blue foxes . More data on serumbiochemistry is needed. However, it can be expected that small blood count together with serumbiochemistry profile is a potential test combination for analysis of animal welfare and health.
Glucose is the main and rapid precursor of energy in animal body. Glucose is the circulating carbohydrate of blood stream and energy yielding nutrient (Cox and Nelson, 2004). The present study showed a decrease in serum glucose level. The decrease were not significant in all groups. A significant difference (P<0.05) were observe between Control (H) and F group, and G and F group as shown in Table 1. Decrease in serum glucose was found in rabbits fed with oxidized ghee. The increase level of LDL, total cholesterol and TG may decrease the concentration of serum glucose level (Zeb and Mehmood, 2012). Previous studies have suggested a decrease in serum glucose level of oxidized tallow fed rabbits. Chances of hypoglycemic condition increases with oxidation and hence weight loss occurs (Zeb and ur Rahman, 2012).
value. The use of frog meal may augment the problem of competitiveness and high cost of conventional protein ingredient like fish meal. However, measuring the blood metabolites and constituents of birds fed frog meal based- diets can be used as basis for comparison to fish meal. Blood is an important index in determining the nutritional and health status of the animals. It is based on this background information that the present study was conducted to evaluate the effect of substituting frog meal for fish meal on serum biochemical and haematological parameters of broiler chickens.
is shown in Table 3. Enzymes, due to cell damage or destruction leaked out into the surrounding fluids and finally into the blood and thereby increasing their concentration in the blood . In this study, the total protein, creatinine, and bilirubin were not affected (P>0.05) by MLM supplementation. This suggests that supplementing the rabbits' diet with MLM as from 35 days of age to 119 days of age did not affect the production of protein, nor produced renal/cardiovascular disorders, neither does it caused liver damage . The reduction in serum cholesterol levels of the rabbits with increased MLM supplementation level and significant (P<0.05) reduction of cholesterol level (P<0.05) at 0.8% and 1.2% dietary MLM supplementation (Diets 3 and 4) may be a result of decreased uptake of cholesterol due to the dietary treatment applied in this study. This could be the reflection of activities of some phytochemicals present in MLM that are interfering with the normal cholesterol absorption in the rabbits. For instance, a high level of saponin has been reported to reduce cholesterol absorption .
Blood samples of six fish/groups were collected randomly from the caudal vein using a vacutainer fitted 5 mL on days 30 and 60. For blood sampling, fish were anaesthetized with MS222 (Sigma Aldrich, Steinheim, Ger- many) . They were well wiped and cleaned in order to avoid mucus mixing into the blood, and blood was taken from the fish through the caudal vein by a 2.5-mL plastic syringe without harming the fish  Then, 200 µL of blood was transferred to ethylenediaminetetraacetic acid (EDTA) tubes (BD, Oxford, UK) for hematolog- ical analysis. The other 600 µL of blood was harvested in plastic biochemistry tubes (Vacutest Kima s.r.l., Piove di Sacco, Italy). After the blood was coagulated, the tubes were centrifuged at 4000 × g for 10 min for serum separation, which was stored below −20˚C .
The serum biochemical indices of WAD goat fed cassava peel enriched with supplemental nitrogen are presented in Table 4. All the parameters measured were significantly different (P < 0.05) except for globulin, ALT, and urea. The value for total protein and globulin ranged between 4.10– 5.18 (g/dl) and 2.20–3.03 (g/dl), respectively, and were significantly (P < 0.0.05) different with T1 and T4 having significantly higher values. These observed values fell within the range as reported by Opara et al.  Creatinine had significant (P < 0.05)