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Serum deprivation

A novel role of vitronectin in promoting survival of mesenchymal stem cells under serum deprivation stress

A novel role of vitronectin in promoting survival of mesenchymal stem cells under serum deprivation stress

... under serum deprivation condition in ...under serum-deprived ...under serum deprivation was partially recovered in the p65 esiRNA- treated samples ...

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Identification of MicroRNAs Involved in Hypoxia- and Serum Deprivation-Induced Apoptosis in Mesenchymal Stem Cells

Identification of MicroRNAs Involved in Hypoxia- and Serum Deprivation-Induced Apoptosis in Mesenchymal Stem Cells

... The use of bone marrow mesenchymal stem cell- (MSC) transplantation therapy for car- diac diseases is limited due to poor survival of implanted cells. MicroRNAs (miRNAs) have been reported to be involved in regulating ...

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The Small GTPase Rac Suppresses Apoptosis Caused by Serum Deprivation in Fibroblasts

The Small GTPase Rac Suppresses Apoptosis Caused by Serum Deprivation in Fibroblasts

... To confirm whether cells died by apoptosis, we performed the terminal deoxynucleotidyl transferase- mediated deoxyuridine nick end-labeling (TUNEL) assay, which reveals DNA breakage. We observed an increase in TUNEL ...

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Increased viability of fibroblasts when pretreated with ceria nanoparticles during serum deprivation

Increased viability of fibroblasts when pretreated with ceria nanoparticles during serum deprivation

... HDF cells at 5,000 cells/well seeded on 96-well plates went through the same incubation process described in the section “Serum Starvation”. Then, after 72 hours of stress, the MTS Cell Proliferation Assay was ...

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The Na+–H+exchanger 1 induces cytoskeletal changes involving reciprocal RhoA and Rac1 signaling, resulting in motility and invasion in MDA MB 435 cells

The Na+–H+exchanger 1 induces cytoskeletal changes involving reciprocal RhoA and Rac1 signaling, resulting in motility and invasion in MDA MB 435 cells

... by serum deprivation. To examine the effect of RhoA and Rac1 on serum deprivation-induced motility and invasive ability of the cells, MDA-MB-435 cell monolayers were left in fresh whole growth ...

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Original Article Neuroprotective effects of genistein-3’-sodium sulfonate in a rat middle cerebral artery occlusion model: roles of enhancing antioxidant ability and regulating NO/NOS system

Original Article Neuroprotective effects of genistein-3’-sodium sulfonate in a rat middle cerebral artery occlusion model: roles of enhancing antioxidant ability and regulating NO/NOS system

... The present study investigated whether GSS can enhance the antioxidant ability of brain tissue in a middle cerebral artery occlusion (MCAO)/reperfusion (MCAO/R) model, a reli- able method for studying reversible regional ...

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A novel cytoprotective peptide protects mesenchymal stem cells against mitochondrial dysfunction and apoptosis induced by starvation via Nrf2/Sirt3/FoxO3a pathway

A novel cytoprotective peptide protects mesenchymal stem cells against mitochondrial dysfunction and apoptosis induced by starvation via Nrf2/Sirt3/FoxO3a pathway

... Methods: MSCs were subjected to serum deprivation and CHBP of graded concentrations was administered. Cell viability and apoptosis were detected by CCK-8, Annexin V/PI assay and Hoechst staining. ROS ...

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Pharmacokinetics of the Yougui pill in experimental autoimmune encephalomyelitis model rats and its pharmacological activity in vitro

<p>Pharmacokinetics of the Yougui pill in experimental autoimmune encephalomyelitis model rats and its pharmacological activity in vitro</p>

... in serum-deprived SH-SY5Y ...by serum deprivation followed with 5% YGP-containing serum, mixed standards and YGP extract for 24 hrs, respectively, and assessed ...

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The influence of serum, glucose and oxygen on intervertebral disc cell growth in vitro: implications for degenerative disc disease

The influence of serum, glucose and oxygen on intervertebral disc cell growth in vitro: implications for degenerative disc disease

... nutritional deprivation may limit the capacity of IVD cells to ...of serum, glucose, or oxygen has a variable influence on their growth and ...survival. Serum supplementation was required for ...

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Original Article QKI-5 regulates FOXO1 to inhibit apoptosis during myocardial ischemia and reperfusion injury in H9C2 myocardiocytes

Original Article QKI-5 regulates FOXO1 to inhibit apoptosis during myocardial ischemia and reperfusion injury in H9C2 myocardiocytes

... and serum deprivation, followed by 6 h of ...the deprivation of culture medium alone could slightly inhibit QKI-5 expres- sion, but such intervention showed no effects on QKI-6 ...

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Autophagy in rat annulus fibrosus cells: evidence and possible implications

Autophagy in rat annulus fibrosus cells: evidence and possible implications

... the serum-free ...under serum starvation conditions, whereas there was a significant increase in both autop- hagy and apoptosis incidence of cells after 24-hour serum ...of serum starvation. ...

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The high affinity IgE receptor (FcεRI) blocks apoptosis in normal human monocytes

The high affinity IgE receptor (FcεRI) blocks apoptosis in normal human monocytes

... Monocytes have a limited life span, and their homeostasis is regulated by apoptosis in vivo. When cultured in the absence of appropriate exogenous stimuli, they undergo apoptosis, but under the influence of survival ...

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Hypodipsic hypernatremia syndrome in an adult with polycythemia: a case report

Hypodipsic hypernatremia syndrome in an adult with polycythemia: a case report

... of serum osmolality is the serum sodium concentration, changes in the concentration of the latter reflect changes in serum ...in serum osmolality by stimu- lating or inhibiting the sensation ...

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Geographical epidemiology of health and overall deprivation in England, its changes and persistence from 2004 to 2015: a longitudinal spatial population study.

Geographical epidemiology of health and overall deprivation in England, its changes and persistence from 2004 to 2015: a longitudinal spatial population study.

... health-related deprivation strongly persisted at the LSOA ...overall deprivation, with regional levels converging towards the English ...health-related deprivation (and within each of its four ...

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MATERNAL DEPRIVATION

MATERNAL DEPRIVATION

... fancy; aim attempt at their etiologic classification. Some empirical findings and hypotheses on three of its manifestations in the first year of life. : Effects of early institutional ca[r] ...

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THE EFFECT OF SALT DEPRIVATION ON THE URINARY CONCENTRATING MECHANISM IN THE DOG

THE EFFECT OF SALT DEPRIVATION ON THE URINARY CONCENTRATING MECHANISM IN THE DOG

... In salt-deprived dogs with normal serum sodium the same amount of sodium was excreted at any given urine flow as in sodium-fed dogs, suggesting that during salt deprivation normal amount[r] ...

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POSTOPERATIVE POTASSIUM DEFICIT AND METABOLIC ALKALOSIS  THE PATHOGENIC SIGNIFICANCE OF OPERATIVE TRAUMA AND OF POTASSIUM AND PHOSPHORUS DEPRIVATION

POSTOPERATIVE POTASSIUM DEFICIT AND METABOLIC ALKALOSIS THE PATHOGENIC SIGNIFICANCE OF OPERATIVE TRAUMA AND OF POTASSIUM AND PHOSPHORUS DEPRIVATION

... THE EFFECTS OF POTASSIUM AND PHOSPHORUS DEPRIVATION AND OF OPERATIVE TRAUMA ON THE NITROGEN, PHOSPHORUS, AND CALCIUM BALANCES; ON THE URINARY CALCIUM EXCRETION; AND ON THE SERUM PHOSPHOR[r] ...

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Manifestation of Compensatory Behavior among Preschool Children with Trust Deprivation

Manifestation of Compensatory Behavior among Preschool Children with Trust Deprivation

... “trust deprivation” [1]. Deprivation of social trust of the children living in the children's homes affects emotional, personal and behavioral spheres of a ...trust deprivation is the cause of a ...

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The effect of social isolation in sensory deprivation on ego-functioning and perception.

The effect of social isolation in sensory deprivation on ego-functioning and perception.

... deprivation was required for Ss to experience the intrusion of primary process in the deprivation situation. Thus, Ss had been able, up to the point of release, to structure the situation meaningfully ...

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Attitudes Towards Immigrants and Relative Deprivation: The Case of a Middle Income Country

Attitudes Towards Immigrants and Relative Deprivation: The Case of a Middle Income Country

... relative deprivation within comparison, rather than reference groups, that is, when a population of natives itself can be sub-divided into groups within which income comparisons are made, there will be group of ...

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