Shikimate Pathway
Comprehensive database of Chorismate synthase enzyme from shikimate pathway in pathogenic bacteria
... A promising drug target for bacterial pathogenic diseases could be developed by blocking any enzymes of this pathway. Designing inhibitors for this reaction would greatly facilitate researchers to block multiple ...
7
Enhanced biosynthesis of arbutin by engineering shikimate pathway in Pseudomonas chlororaphis P3
... than 1.5 g/L 4-HBA was produced using glycerol as a carbon source [17]. XanB2 is an ideal enzyme for design- ing arbutin biosynthesis pathway, and therefore it was employed to generate 4-HBA for arbutin synthesis. ...
14
Design of Novel Antimycobacterial Molecule Targeting Shikimate Pathway of Mycobacterium tuberculosis
... tuberculosis, shikimate pathway is essential for amino acid biosynthesis, siderophores formation to overcome starvation, to survive in low oxygen conditions as well as for pathogen’s virulence and ...
10
Unraveling the kinetic diversity of microbial 3-dehydroquinate dehydratases of shikimate pathway
... From all retrieved 459 DHQases, 38 were further se- lected (Table 2).The selection was carried out according to the following criterions: 1) Both type I and type II DHQases were included, and the majority was bacterial ...
9
Evolutionary Origins of the Eukaryotic Shikimate Pathway: Gene Fusions, Horizontal Gene Transfer, and Endosymbiotic Replacements
... AroDE in plants is an HGT and is not indicative of a planc- tomycete-plastid progenitor sisterhood. The final possible ex- ample of HGT in the Plantae shikimate pathway comes from the phylogeny of AroE ...
15
The acanthamoeba shikimate pathway has a unique molecular arrangement and is essential for aromatic amino acid biosynthesis
... the shikimate pathway. Rescue was determined by measuring the percentage alamarBlue reduction of test cultures compared to those of untreated controls. The addition of single aromatic amino acids (Phe, Trp ...
39
Metabolic flux responses to genetic modification for shikimic acid production by Bacillus subtilis strains
... several microorganisms, particular Escherichia coli, for SA accumulation [6-9]. One strategy was to increase the supply of PEP and/or E4P, thus more carbon substrate channeled into SA synthesis. For examples, the PEP- ...
11
The conserved Lysine69 residue plays a catalytic role in Mycobacterium tuberculosis shikimate dehydrogenase
... the shikimate pathway flux in ...whether shikimate dehydrogenase has a low flux control coefficient because mutation of Lys69 would be one way of gaining resistance to this enzyme’s ...
7
Production of hydroxycinnamoyl-shikimates and chlorogenic acid in Escherichia coli: production of hydroxycinnamic acid conjugates
... . Shikimate and 3-dehydroquinate, which are acyl group acceptors for hydroxycinnamate-CoA shikimate trans- ferases (HST) and hydroxycinnamate-CoA quinate transferases (HQT), respectively, can be synthesized ...
11
Genetic engineering of Pseudomonas chlororaphis GP72 for the enhanced production of 2-Hydroxyphenazine
... the shikimate pathway, into PCA, which is the synthesis substrate of the phena- zine derivatives [13, ...the shikimate pathway, which is used as substrate in the synthesis of oseltamivir ...
12
Substrate analogues as mechanistic probes for 3 Deoxy D arabino heptulosonate 7 phosphate synthase and 3 Deoxy D manno octulosonate 8 phosphate synthase : a dissertation presented in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Biochemistry at Massey University, Palmerston North, New Zealand
... re shikimate pat hw ...the shikimate pathway is responsible for 20 - 30 % of the carbon fl ux, suggesting that the first step of the pathway, DAH7P synthase, is important i n the control of ...
228
Engineering and systems-level analysis of Pseudomonas chlororaphis for production of phenazine-1-carboxamide using glycerol as the cost-effective carbon source
... Shikimate pathway, a bridge between primary and sec- ondary metabolism, links the carbohydrate metabolism to the biosynthesis of phenazines [29, ...mate pathway starts with DAHP-synthase-mediated ...
15
Novel technologies combined with traditional metabolic engineering strategies facilitate the construction of shikimate-producing Escherichia coli
... into shikimate pathway is only around ...the shikimate pathway ...the shikimate pathway interme- diate DAHP ...of shikimate, is derived from the pentose phosphate ...in ...
8
Transcriptome analysis of a phenol-producing Pseudomonas putida S12 construct: Genetic and physiological basis for improved production
... tion in P. putida S12TPL3 (Fig. 2) in order to gain further insight into the enhanced phenol production characteristics of this strain. The hpd and pobA genes (locus tags PP3433 and PP3537, respectively) are responsible ...
9
Metabolic engineering of Escherichia coli BL21 (DE3) for de novo production of l-DOPA from d-glucose
... Microbial fermentation for l-DOPA production has pre- viously been focused on the catalyzation of l-tyrosine by tyrosinase [7] or through catechol and pyruvate from tyrosine phenol-lyase (TPL) [49–51]. The de novo pro- ...
10
Immunohistochemical localization of enzymes related to lignin biosynthesis in the primary xylem of hybrid aspen
... The spatial localization of 3-deoxy-D-arabino- heptulosonate 7-phosphate synthase (DAHPS), which is one of the key enzymes in the shikimate pathway, was tightly cor[r] ...
10
Insilico Identification of Novel Inhibitors for Shikimate Kinase from Mycobacterium Tuberculosis using Natural and Synthetic Ligands
... disease. Shikimate pathway plays indispensable role in synthesis of aromatic compounds and many other essential nutrients in plants and microorganisms (bacteria, fungi and algae) It involved series of ...
10
Isolation of alpha linolenic acid from Sutherlandia frutescens and its inhibition of Mycobacterium tuberculosis’ shikimate kinase enzyme
... the shikimate bio- synthetic pathway from ...the pathway in which it is involved only takes place in microbes and some plants but is absent in mammals ...The shikimate pathway involves ...
8
Down-regulation of p-coumaroyl quinate/shikimate 3′-hydroxylase (C3′H) and cinnamate 4-hydroxylase (C4H) genes in the lignin biosynthetic pathway of Eucalyptus urophylla × E. grandis leads to improved sugar release
... in H monomers is consistent with impeding the conver- sion of p-coumaroyl quinate/shikimate to caffeoyl qui- nate/shikimate by down-regulation of the C3 ′ H gene, reducing the formation of the monolignols ...
10
Dynamic simulation of red blood cell metabolism and its application to the analysis of a pathological condition
... thesis pathway can partially compensate for the lowered GSH concentrations resulting from G6PD deficiency, and that the concentration of GSSG can be kept at a very low level due to the active export ...
11