Shotgun Proteomics
White Spot Syndrome Virus Proteins and Differentially Expressed Host Proteins Identified in Shrimp Epithelium by Shotgun Proteomics and Cleavable Isotope-Coded Affinity Tag
... Human epithelial proteomics has provided powerful infor- mation on the relationships between biological molecules and disease mechanisms (58). Here we have generated a data set of shrimp epithelial proteins ...
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GO Explorer: A gene-ontology tool to aid in the interpretation of shotgun proteomics data
... Shotgun proteomics is a strategy capable of identifying thousands of proteins in complex ...a shotgun pro- teomics technique capable of identifying thousands of proteins in proteolytically digested ...
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Quantifying raft proteins in neonatal mouse brain by 'tube-gel' protein digestion label-free shotgun proteomics
... tures. Proteomics profiling of either protein mixtures frac- tionated by 1DE or unfractionated protein mixtures by protease digestion and LC-MS/MS analysis has become increasingly ...
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Altered proteolytic events in experimental autoimmune encephalomyelitis discovered by iTRAQ shotgun proteomics analysis of spinal cord
... previous shotgun proteomics study using isobaric tags for relative and absolute quantification (iTRAQ) to compare the proteomes of normal and EAE rat lumbar spinal ...
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Michalski, Annette (2012): Overcoming challenges of shotgun proteomics. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... and shotgun proteomics, we also evaluated a top-down ...bottom-up shotgun proteomics, as the sequences often lack a tryptic cleavage site or the obtained peptides are too ...conventional ...
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A quantitative shotgun proteomics analysis of germinated rice embryos and coleoptiles under low-temperature conditions
... quantitative shotgun proteomics analysis of low-temperature resistant and susceptible em- bryos and coleoptiles in both cold and control tempera- tures, we identified a total of 2626 non-redundant proteins, ...
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What’s in SWAP? Abundance of the principal constituents in a soluble extract of Schistosoma mansoni revealed by shotgun proteomics
... In spite of this long-established usage very little is known about its precise composition. The first prote- omic study of S. mansoni, using 2D-gel technology and peptide-mass fingerprinting, provided a comparison of ...
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Shotgun proteomics implicates protease inhibition and complement activation in the antiinflammatory properties of HDL
... Shotgun proteomics identifies distinct protein families in HDL. We first confirmed that liquid chromatography–electrospray ionization– tandem MS (LC-ESI-MS/MS) analysis reproducibly identifies the same ...
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Beck, Scarlet Svenja Anna-Maria (2016): Novel quadrupole time-of-flight mass spectrometry for shotgun proteomics. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... Recent advances in proteomic technologies have made MS-based proteomics a central research tool. However, truly complete proteomes are still elusive, mainly due to the high complexity and dynamic range of ...
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MUMAL: multivariate analysis in shotgun proteomics using machine learning techniques.
... The shotgun strategy by means of LC-MS/MS is currently the standard method for analyzing complex ...to shotgun DNA sequen- cing, where small DNA molecules are computationally assembled into the continuous ...
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Enhanced detection method for corneal protein identification using shotgun proteomics
... proteins, proteomics procedures have typically involved the pulverization of the entire cornea prior to ...separate shotgun proteomics using liquid chromatography/mass spectrometry results in ...
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SILAC labeling coupled to shotgun proteomics analysis of membrane proteins of liver stem/hepatocyte allows to candidate the inhibition of TGF-beta pathway as causal to differentiation
... Here, we aimed to identify RLSC functional elements underlying either self-renewal or differentiation toward hepatocytes. We therefore compared the membrane pro- tein profile of self-renewing RLSCs with those of RLSCdH ...
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Quantitative shotgun proteomics distinguishes wound-healing biomarker signatures in common carp skin mucus in response to Ichthyophthirius multifiliis
... Ichthyophthirius multifiliis is a ciliated protozoan parasite recognized as one of the most pathogenic diseases of wild and cultured freshwater fish. Fish skin mucus plays a significant role against invading pathogens. ...
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A label-free quantitative shotgun proteomics analysis of rice grain development
... label-free shotgun proteomic approach, we were able to identify a rice grain proteome at large scale where physiochemical properties of the identified pro- teins were unbiased and to monitor protein expression ...
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Shotgun proteomic analysis of mulberry dwarf phytoplasma
... more proteomics techniques (such as two-dimensional polyacrylamide gel electrophoresis (2- DE), matrix-assisted laser desorption ionisation-time-of- flight mass spectrometry, liquid chromatography tandem mass ...
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Interleukin-6 Induced “Acute” Phenotypic Microenvironment Promotes Th1 Anti-Tumor Immunity in Cryo-Thermal Therapy Revealed By Shotgun and Parallel Reaction Monitoring Proteomics
... Shotgun data-dependent acquisition (DDA) MS Approximately 0.5 µg of glycopeptides from each fraction were separated in a 2-h gradient on a 15-cm long (75 µm inner diameter) PicoFrit Self-Pack Column (360 µm OD, 75 ...
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Derivation of HLA types from shotgun sequence datasets
... HLAminer is the implementation of a strategy for auto- mated HLA typing directly from NGS data sets. It is a fundamentally different approach compared to conven- tional methods that all rely on first amplifying HLA ...
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INSTRUCTION MANUAL FOR 12 GAUGE OVER & UNDER SHOTGUN
... Before disassembling, ensure your shotgun is unloaded and pointed in a safe direction. Move the barrel latch downward to its rear most position. Move the top lever to the extreme right to open the shotgun. ...
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Principles and Applications of Proteomics
... Protein Identification Using Peptide Mass Fingerprinting (MALDI-TOF Data). Experimental Proteolytic[r] ...
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High Throughput Proteomics
... Mascot Input File Mascot Search Engine Mascot Search Results Data reduction Search parameters MS Data. Parse results into database[r] ...
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