Signal Peptide
Signal peptide replacement resulted in recombinant homologous expression of laccase Lcc8 in Coprinopsis cinerea
... native signal peptide of lcc8 the signal peptide of lcc1 was ...Lcc1 signal peptide. The * marks the signal peptide cleavage side ...
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Evaluating the Effect of Protein A Signal Peptide on Extracellular Expression of Recombinant Hirudin in E.Coli
... In order to investigate the expression of hirudin, the expression sample was compared through electrophoresis on SDS-PAGE gel with negative expression controls that include an E.coli JM107 sample transformed with pEZZ18 ...
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The Signal Peptide of a Simple Retrovirus Envelope Functions as a Posttranscriptional Regulator of Viral Gene Expression
... In the same experimental set, we also quantified the levels of JSRV Gag present in cell lysates and supernatants of trans- fected cells by Western blotting and chemifluorescence (Fig. 7D). The influence of JSE-SP on ...
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A novel strategy to improve protein secretion via overexpression of the SppA signal peptide peptidase in Bacillus licheniformis
... release. Signal peptides play an important role in protein secretion, these peptide sequences are cleaved from the mature protein by signal peptidase I during the late stage of the secretion ...
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Improving N terminal protein annotation of Plasmodium species based on signal peptide prediction of orthologous proteins
... Interestingly, signal peptide pre- diction patterns that concur with the phylogeny of Plasmodium species were significantly over represented in these groups, which argues in favor of biological nov- elties ...
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Requirements for Mouse Mammary Tumor Virus Rem Signal Peptide Processing and Function
... long signal peptide (7, ...cellular signal pep- tides are to bind the signal recognition particle (SRP) and direct mRNAs to the ER membrane for translation ...by signal peptidase in the ...
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The Signal Peptide of the Ebolavirus Glycoprotein Influences Interaction with the Cellular Lectins DC-SIGN and DC-SIGNR
... The signal sequence impacts EBOV-GP modification with high-mannose ...the signal peptide might modulate to what extent these carbohydrates are incorporated into EBOV-GP and that dif- ferential ...
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Use of a Sec signal peptide library from Bacillus subtilis for the optimization of cutinase secretion in Corynebacterium glutamicum
... the signal peptide and target (pre-) protein to be recognized by signal peptidase ...the signal recognition parti- cle (SRP), transported to the Sec translocon in the cyto- plasmic membrane ...
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Mapping the Landscape of the Lymphocytic Choriomeningitis Virus Stable Signal Peptide Reveals Novel Functional Domains
... stable signal peptide (SSP), which is cotranslationally cleaved by signal peptidase and followed by the GP precursor GPC (residues 59 to 498), which is further processed into GP1 (residues 59 to 265) ...
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Role of the Stable Signal Peptide of Junín Arenavirus Envelope Glycoprotein in pH-Dependent Membrane Fusion
... The arenaviruses are enveloped viruses whose genome con- sists of two single-stranded RNA molecules that encode am- bisense expression of the four viral proteins (8, 14). Because the envelope glycoprotein (GP-C) mediates ...
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Conformational determinants necessary for secretion of Paecilomyces thermophila β-1,4-xylosidase that lacks a signal peptide
... a signal peptide (Teng et ...of signal peptide-lacking proteins in various bacteria, there is no conclusive and universally applicable pathway identified so ...of signal ...
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Rubella Virus E2 Signal Peptide Is Required for Perinuclear Localization of Capsid Protein and Virus Assembly
... The signal peptide that initiates translocation of E2 into the lumen of the endoplasmic reticulum remains attached to the carboxy terminus of the capsid protein after cleavage by signal ...E2 ...
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Myristoylation of the Arenavirus Envelope Glycoprotein Stable Signal Peptide Is Critical for Membrane Fusion but Dispensable for Virion Morphogenesis
... Arenaviruses are responsible for severe and often fatal hemorrhagic disease. In the absence of effective antiviral therapies and vaccines, these viruses pose serious threats to public health and biodefense. Arenaviruses ...
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Secretory signal peptide modification for optimized antibody-fragment expression-secretion in Leishmania tarentolae
... Secretory signal peptides (SPs) are well-known sequence motifs targeting proteins for translocation across the endoplasmic reticulum ...the signal peptide cleavage site as well as changes of ...
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Mutation of the signal peptide encoding region of the preproparathyroid hormone gene in familial isolated hypoparathyroidism
... the signal peptide-encoding region of a preproPTH gene in one FIH kindred and demonstrated a functional defect caused by the ...the signal sequence constitutes a novel pathophysiologic mechanism in ...
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Modified FGF4 Signal Peptide Inhibits Entry of Herpes Simplex Virus Type 1
... a peptide with antivi- ral activity, which blocks the entry and fusion steps of HSV-1 ...This peptide consists of the 16-amino-acid FGF4 signal peptide with an RRKK tetramer attached to its ...
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Signal Peptide and Helical Bundle Domains of Virulent Canine Distemper Virus Fusion Protein Restrict Fusogenicity
... the signal peptide between both F CDV strains or replacing it with an exogenous signal peptide, we demonstrated that this domain controlled intracellular and consequently cell surface protein ...
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Drosophila Signal Peptide Peptidase Is an Essential Protease for Larval Development
... melanogaster Signal peptide peptidase gene (Spp) that encodes a multipass transmembrane aspartyl ...human signal peptide peptidase (SPP) and is distantly related to the ...model signal ...
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A novel feature extraction method by compressive sensing for signal peptide
... of signal peptide that can truly reflect the intrinsic attributes of the ...every signal peptide that displays diverse length, we first transformed the original sequence into Markov transition ...
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Characterization of the Glycoprotein Stable Signal Peptide in Mediating Pichinde Virus Replication and Virulence
... stable signal peptide (SSP) is unique among signal peptides in that it is an integral component of the ma- ture glycoprotein complex (GPC) and plays important roles not only in GPC expression and ...
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