siRNA nanoparticles
Albumin pre-coating enhances intracellular siRNA delivery of multifunctional amphiphile/siRNA nanoparticles
... EHCO/siRNA nanoparticles modified with arginine-alanine-aspartic acid (RAD)-PEG- Mal, and PEG-Mal, were prepared as control ...the nanoparticles was in the range of 50–70 nm, as measured by dynamic ...
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Chitosan/siRNA Nanoparticles Targeting Cyclooxygenase Type 2 Attenuate Unilateral Ureteral Obstruction-induced Kidney Injury in Mice
... (siRNA) nanoparticles to knockdown COX-2 specifically in macrophages to prevent kidney injury induced by unilateral ureteral obstruction ...chitosan/siRNA nanoparticles accumulated in mac- ...
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Megalin-Mediated Specific Uptake of Chitosan/siRNA Nanoparticles in Mouse Kidney Proximal Tubule Epithelial Cells Enables AQP1 Gene Silencing
... of siRNA to the PTECs raises an interesting question: How are the siRNA/chitosan nanocom- plexes filtered in view of the glomerular sieving threshold? One possibility is that a fraction of chi- ...
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Targeting Tumors and the Kidney with siRNA Nanoparticles and Evaluation of Extracellular MicroRNA-based Methodologies to Track Their Activity
... naked siRNA and methods of administration not compatible with clinical ...the siRNA mechanism of action by using a modified 5’-RACE (rapid amplification of cDNA ends) PCR technique providing positive ...
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Overcoming multidrug resistance through inhalable siRNA nanoparticles-decorated porous microparticles based on supercritical fluid technology
... the nanoparticles, as well as PMs, was characterized by field emission scanning electron microscopy (FE-SEM, S-4800 UHR; Hitachi, Tokyo, ...CS nanoparticles and siRNA-CS nanoparticles were ...
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<p>p66shc siRNA Nanoparticles Ameliorate Chondrocytic Mitochondrial Dysfunction in Osteoarthritis</p>
... p66shc siRNA in the MIA-induced OA ...p66shc siRNA into joints, we used poly(lactic-co-glycolic acid) PLGA copolymers, which are biodegradable and bio- compatible with humans, and have been approved by the ...
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In vivo therapeutic efficacy of TNFα silencing by folate-PEG-chitosan-DEAE/siRNA nanoparticles in arthritic mice
... protects siRNA against nuclease destruction, its ligands facili- tate siRNA uptake via cell surface receptors, and it provides improved solubility at neutral pH with transport of its load into target ...
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Efficient delivery of NF-κB siRNA to human retinal pigment epithelial cells with hyperbranched cationic polysaccharide derivative-based nanoparticles
... derivative/cy5-siRNA nanoparticles at different weight ratios (w/w = 5, w/w = 10, and w/w = 20) were prepared and incubated with cells in each well at a cy5-siRNA final concentration of 50 ...
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Chitosan-based nanoparticles for survivin targeted siRNA delivery in breast tumor therapy and preventing its metastasis
... as siRNA delivery carrier was synthesized successfully, which could encapsulate siRNA ...PEG-CS/siRNA nanoparticles were conveniently prepared via a single-step ionic gelation method with a ...
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Comparative Evaluation of siRNA-loaded Chitosan Tripolyphosphate/Dextran Sulfate Nanoparticles Prepared by Adsorption Method: In Vitro Characterizations
... of siRNA- loaded CS-TPP/DS nanoparticles by increasing the CS concentration from ...CS-TPP- siRNA nanoparticles had the smallest particle size at all concentrations studied ...
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Novel functionalized nanoparticles for tumor-targeting co-delivery of doxorubicin and siRNA to enhance cancer therapy
... selenium nanoparticles (RGDfC-SeNPs) to load chemotherapeutic doxorubicin (DOX) and Nanog ...DOX nanoparticles (~12 nm) were confirmed by both dynamic light scattering and transmis- sion electron ...with ...
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Polyphosphoester nanoparticles as biodegradable platform for delivery of multiple drugs and siRNA
... the nanoparticles were determined (Figure 3). The size of the nanoparticles prepared at dif- ferent N/P ratios ranged from 170 to 400 nm (Figure ...of nanoparticles initially increased to 390 ...3B). ...
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Chaperonin–dendrimer conjugates for siRNA delivery
... The change in molar mass of THS subunits due to PAMAM conjugation was analyzed with SDS–PAGE ( Figure 3 a). The THS subunits have a molecular weight of ≈ 58 kDa. PAMAM exhibits a smeared band with a maximum slightly ...
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Synthetic poly(ester amine) and poly(amido amine) nanoparticles for efficient DNA and siRNA delivery to human endothelial cells
... of siRNA makes quick polymer degradation or nucleic acid release more crucial than in DNA delivery, where slower degradation could protect the plasmid for a longer time as it is trafficked towards the ...for ...
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I Synthesis and Proton Conductivity Studies of Mesostructured Organosilicates and Bitriazole Polymer Composites II Targeted Nanoparticles for siRNA Delivery
... The use of therapeutic nucleic acids such as short interfering RNAs (siRNA) to silence disease-causing genes has enjoyed limited success due to numerous biological barriers. Those specifically inherent to siRNAs ...
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High-Yield Synthesis of Monomeric LMWP(CPP)-siRNA Covalent Conjugate for Effective Cytosolic Delivery of siRNA
... fluorescent-labeled siRNA not only entered the cells but, giving sufficient time, also destined preferably towards the nucleus compartment, apparently attributing to the nucleus localization function by the ...
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<p>Codelivery of GRP78 siRNA and docetaxel via RGD-PEG-DSPE/DOPA/CaP nanoparticles for the treatment of castration-resistant prostate cancer</p>
... using siRNA to downregu- late the expression of GRP78 include high speci fi city and low toxicity to normal cells, but an ef fi cient siRNA delivery vector is in urgent need as free siRNA can be easily ...
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Enhanced therapeutic effect of Adriamycin on multidrug resistant breast cancer by the ABCG2-siRNA loaded polymeric nanoparticles assisted with ultrasound
... get nanoparticles with hydrophilic shell and hydrophobic core, suitable for carrying various genes ...charged siRNA to increase the entrapment efficiency ...as siRNA deliver system assistant with ...
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Targeted co-delivery of Beclin 1 siRNA and FTY720 to hepatocellular carcinoma by calcium phosphate nanoparticles for enhanced anticancer efficacy
... the siRNA and making the coated cores soluble in organic solvent; a suitable neutral or cationic lipid was used as the outer leaflet lipid to form an asymmetric lipid bilayer ...delivered siRNA in vitro and ...
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sirna Duplexes & RNAi Explorer
... RNAi Explorer from Gene Link is a series of product and services to aid researchers in exploring RNA interference. The online search and design algorithms for siRNA or shRNA are developed based on current known ...
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