siRNA transfection
siRNA transfection in larvae of the barnacle Amphibalanus amphitrite
... study, siRNA against p38 MAPK was synthesized and then transfected into ...of siRNA transfection on the p38 MAPK level were hardly detectable in the cyprids when they were transfected at the nauplius ...
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Highly efficient siRNA transfection in macrophages using apoptotic body-mimic Ca-PS lipopolyplex
... for transfection of the hard-to-transfect macrophages due to the hypothesis that PS can effect a much more effective uptake of liposomes, compared to routine transfection reagents, by macrophages in a way ...
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Transforming Growth Factor-β1 Downregulates Vascular Endothelial Growth Factor-D Expression in Human Lung Fibroblasts via the Jun NH2-Terminal Kinase Signaling Pathway
... VEGF-D siRNA exhibited a significant increase in the percentage of S-phase cells compared with control siRNA transfection, indicating that VEGF-D knockdown promoted the transition from G1 to S phase ...
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ARID3B : a novel regulator of the Kaposi’s sarcoma associated herpesvirus lytic cycle
... ARID3B siRNA-treated cells compared to scramble ...ARID3B siRNA or scramble control siRNA transfection of iSLK-BAC16 cells followed by reactivation of the lytic cycle for 72 h led to an ...
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Original Article Aberrant NEAT1 promotes migration in endometrial cancer and as marker of poor prognosis
... using siRNA, and the qRT-PCR re- sults verified that the siRNA transfection significantly re- duced NEAT1 expression (*in- dicates P < ...the siRNA group had a markedly wider wound than the ...
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Stimulation of translation by human Unr requires cold shock domains 2 and 4, and correlates with poly(A) binding protein interaction
... after siRNA transfection, and cells were harvested 6 hours ...Unr siRNA treated ...Unr siRNA (Fig. 4c). The negative control siRNA had no effect on ...
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MicroRNA-486-3p directly targets BIK and regulates apoptosis and invasion in colorectal cancer cells
... Background: MicroRNAs influence almost every genetic pathway and are involved in colorectal cancer (CRC). However, the biological role of miR486-3p in CRC remains to be elucidated. Methods: In this study, miR486-3p ...
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ARID3B: a Novel Regulator of the Kaposi's Sarcoma-Associated Herpesvirus Lytic Cycle
... ARID3B siRNA-treated cells compared to scramble ...ARID3B siRNA or scramble control siRNA transfection of iSLK-BAC16 cells followed by reactivation of the lytic cycle for 72 h led to an ...
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Original Article Roles of MED27 in regulating glioma cells via NF-κB iNOS
... controlled siRNA, and MED27 siRNA transfection ...MED27 siRNA, expression of MED27 protein was decreased, leading to inhibition of U87 cell proliferation or invasion, in addition to enhanced ...
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The Relationship between R&D Investment and Dividend Payment Tax Incentives and Their Role in the Dividend Tax Puzzle
... silencing control (1.5µg), or reagent only was transfected into HeLa cells. Following siRNA transfection, cells were stimulated with IFN-γ for 20 hours to induce transcription of CIITA and MHC II (30). ...
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P Bodies Inhibit Retrotransposition of Endogenous Intracisternal A Particles
... Dicer siRNA and analyzed IAP RTP at 8 days after the initial ...Dicer siRNA transfection and day 4 after the second Dicer siRNA transfection (data not ...
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Inhibition of the AKT pathway in cholangiocarcinoma by MK2206 reduces cellular viability via induction of apoptosis
... used siRNA knockdown of survivin in order to determine the role of survivin in MK2206 treat- ...survivin siRNA transfection and MK2206 treatment resulted in significant reduction in cell viability ...
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TAT-IL-24-KDEL-induced apoptosis is inhibited by survivin but restored by the small molecular survivin inhibitor, YM155, in cancer cells
... the transfection efficiency of TAT-IL-24-KDEL, fluorescein isothiocyanate (FITC) was conjugated to the fused protein for the feasibility of localization ...
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Downregulation of KPNA2 in non small cell lung cancer is associated with Oct4 expression
... KPNA2 siRNA treatment with Thermo Scientific NE-PER Nu- clear and Cytoplasmic Extraction ...KPNA2 siRNA treat- ment, as observed by real-time PCR (Figure ...
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A method for concentrating lipid peptide DNA and siRNA nanocomplexes that retains their structure and transfection efficiency
... (C) sirNa silencing from anionic Prl nanocomplexes (with peptide Y) before or after concentration made at a 4:3:1 molar charge ratio using sirNa targeting luciferase in Neuro-2A-Luc cells at 50 ...following ...
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<p>Effect of RSK4 on Biological Characteristics of Gastric Cancer</p>
... Purpose: Gastric cancer is one of the most common cancers with high mortality. Emerging evidences show that ribosomal s6 kinase4 (RSK4) may be an anti-oncogene in several types of cancers, while its function in GC is ...
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Bmi1 Drives Stem-Like Properties and is Associated with Migration, Invasion, and Poor Prognosis in Tongue Squamous Cell Carcinoma
... As expected, the expression of Bmi1 was inhibited in SP cells after transfection with C-myc siRNA (Figure 5E and S2D); the SP cells transfected with C-myc siRNA also displayed decrea[r] ...
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TRAF6 Affects RAC1 Expression and Apoptosis in SK Hep1 Cells
... RAC1 is a small-molecule G protein that regulates multiple cell cycle, cytoskelet- al reorganization, cell migration, and apoptosis. FADD-dependent TRAIL can promote tumor metastasis through RAC1 and PI3K, and ...
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Silencing of LncRNA ANCR Promotes the Osteogenesis of Osteoblast Cells in Postmenopausal Osteoporosis via Targeting EZH2 and RUNX2
... Fig. 3. Effects of long non-coding RNA anti-differentiation noncoding RNA (lncRNA-ANCR) transfected with siRNA-ANCR (si-ANCR). (A) The effect of lncRNA-ANCR on proliferation evaluated by MTT assay. (B) The effect ...
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CELF1 is Up-Regulated in Glioma and Promotes Glioma Cell Proliferation by Suppression of CDKN1B
... CELF1 siRNA was utilised to specifically knockdown CELF1 mRNA in U87 and U251 cells to decrease CELF1 ex- ...pression. Transfection of U87 and U251 glioma cell lines with an siRNA duplex for 2 d led ...
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