Skin Fibroblasts
Respiratory chain defects in the mitochondria of cultured skin fibroblasts from three patients with lacticacidemia
... cultured skin fibroblasts from three patients with lacticacidemia were found to have low rates of 1-[14C]pyruvate oxidation in the face of normal pyruvate dehydrogenase ...from skin ...
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Complementation studies of isovaleric acidemia and glutaric aciduria type II using cultured skin fibroblasts
... cultured skin fibroblasts, we studied the heterogeneity of inborn errors of leucine metabolism such as isovaleric acidemia (IVA), glutaric aciduria type II (GA II), and multiple carboxylase deficiency ...
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Effects of Cigarette Smoke Extracts on the Growth and Senescence of Skin Fibroblasts In Vitro
... primary skin fibroblasts in vitro, and we investigated the mechanisms by which CS induces cellular senescence in cultured primary skin ...of fibroblasts was inhibited in a dose-dependent ...
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Influence of caffeine and hyaluronic acid on collagen biosynthesis in human skin fibroblasts
... The enzyme responsible for recycling proline for collagen resynthesis is prolidase. Its activity was assessed in cultures of human skin fibroblasts in conditions established in the current experiment. ...
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Hydroxyeicosatetraenoic acid metabolism in cultured human skin fibroblasts Evidence for peroxisomal beta oxidation
... deficient skin fibroblasts from patients with Zellweger's (cerebrohepatorenal) ...normal fibroblasts, reverse phase HPLC indicated that 24% of the 12-HETE radioactivity was converted to one major ...
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Immune complexes containing scleroderma specific autoantibodies induce a profibrotic and proinflammatory phenotype in skin fibroblasts
... SSc-specific antibodies. The first relevant observation that emerged in this work consists of the confirmation of IC content in all PEG-precipitated preparations, with significantly higher IC amounts in the samples from ...
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Uroporphyrin I Stimulation of Collagen Biosynthesis in Human Skin Fibroblasts: A UNIQUE DARK EFFECT OF PORPHYRIN
... human skin fibroblasts were exposed to uroporphyrin I and to 400 nm radiation and the effect of these agents on collagen accumulation by the cells was ...
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Demonstration of 26 hydroxylation of C27 steroids in human skin fibroblasts, and a deficiency of this activity in cerebrotendinous xanthomatosis
... In skin fibroblasts from three patients with cerebrotendinous xanthomatosis CTrX, 26-hydroxylation of C2rsteroids proceeded at a rate of only 0.2-2.5% of healthy controls.. No accumulati[r] ...
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Glycopeptide storage in skin fibroblasts cultured from a patient with alpha mannosidase deficiency
... mannosidosis fibroblasts or for any abnormality in cell surface glycoprotein ...cultured skin fibroblasts may be attributed to the low level of N-aspartyl-beta-glucosamindase (EC ...
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Intracellular and nuclear binding of [3H]dihydrotestosterone in cultured genital skin fibroblasts of patients with severe hypospadias
... genital skin fibroblasts from 10 males who underwent circumcision for phimosis (controls A), 2 patients with 5 alpha-reductase deficiency (controls B), and 11 patients with severe forms of hypospadias of ...
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CD109, a TGF β co receptor, attenuates extracellular matrix production in scleroderma skin fibroblasts
... SSc skin fibroblasts and the demonstrated ability of endogenous CD109 protein to inhibit ECM and CCN2 production, the levels of ECM and CCN2 in SSc fibro- blasts remain ...SSc fibroblasts, which ...
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Resistance to 1,25 dihydroxyvitamin D Association with heterogeneous defects in cultured skin fibroblasts
... with skin fibroblasts cultured from normal subjects or from affected members of six kindreds with rickets and resistance to 1-alpha, 25(OH)2D ...normal fibroblasts the affinity and capacity of ...
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Comparative proteomic analysis of extracellular matrix proteins secreted by hypertrophic scar with normal skin fibroblasts
... of fibroblasts, abnormal deposition of extracellular matrix (ECM) during wound healing, associated with cosmetic, functional, and psychological ...normal skin fibroblasts (NSFs) and HS ...
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miR-27a-containing Exosomes Secreted by Irradiated Skin Keratinocytes Delayed the Migration of Unirradiated Skin Fibroblasts
... of skin fibroblasts play important roles in wound healing [40], thus in this study we investigated whether irradiated HaCaT cells would affect the proliferation and the migration of bystander WS1 ...WS1 ...
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Skin fibroblasts from individuals with Chediak-Higashi Syndrome (CHS) exhibit hyposensitive immunogenic response
... Methods and results: Primary skin fibroblasts from CHS subjects or healthy controls were assessed for genes encoding inflammatory response factors using PCR array. At baseline, we found CD14, IL1R1 and ...
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Sialylation regulates myofibroblast differentiation of human skin fibroblasts
... Sialidase NEU1 has been shown to be involved in epithe- lial to mesenchymal transition in pancreatic cancer due to activation of matrix metalloproteinase 9-EGFR signaling after reduction of EGFR sialylation [34, 35]. ...
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Oxidative stress in skin fibroblasts cultures from patients with Parkinson's disease
... generalised phenomenon. It is of note that complex V has not been usually studied neither in brain nor in per- ipheral tissues from PD patients. Indeed, complex V was not measured in previous studies by our group using ...
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Heme synthetase deficiency in human protoporphyria Demonstration of the defect in liver and cultured skin fibroblasts
... The final step in heme biosynthesis is chelation of porphyrin with Fe++ catalyzed by the mitochondrial enzyme heme synthetase. We have employed a sensitive radiochemical assay for this enzyme, using 59Fe and ...
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Characterization of Deficient Heme Synthase Activity in Protoporphyria with Cultured Skin Fibroblasts
... Heme synthase (ferrochelatase) activity, as determined by the chelation of ferrous iron to protoporphyrin or deuteroporphyrin, is reduced to 10-25% of normal in tissues of patients with protoporphyria. With cultured ...
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Roughness threshold for cell attachment and proliferation on plasma micro-nanotextured polymeric surfaces: The case of primary human skin fibroblasts and mouse immortalized 3T3 fibroblasts
... Bourkoula, A, Constantoudis, V, Kontziampasis, D orcid.org/0000-0002-6787-8892 et al. (4 more authors) (2016) Roughness threshold for cell attachment and proliferation on plasma micro-nanotextured polymeric surfaces: The ...
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