SKOV-3
Original Article Mechanism of SKOV-3 inducing hMSC homing
... hMSC, using cell scratch assay and Boyden- Chamber method. Thus, HGF/c-met signaling played an important role in hMSC motivation and homing [18]. Monocyte chemotactic pro- tein-3 (MCP-3) belongs to the CC ...
11
Original Article MicroRNA-22 suppresses metastasis by targeting SNAI1 in the ovarian cancer cell line SKOV-3
... Abstract: MicroRNAs (miRNAs), a class of small non-coding RNAs, regulate gene expression by targeting specific mRNAs to trigger translational repression or mRNA degradation. MiR-22 is aberrantly expressed in human ...
10
Effect of ovarian cancer ascites on SKOV-3 cells proteome: new proteins associated with aggressive phenotype in epithelial ovarian cancer
... modifying SKOV-3 cells, we proceeded with their proteomic ...ascites-treated SKOV-3, as well as from cells incubated in culture medium were analyzed by 2-DE gel electrophoresis to obtain a ...
12
Original Article ERα and ERβ oppositely regulated plexin B1 expression and migration of ovarian cancer SKOV-3 cells
... The ERα and ERβ highly expressed vectors were constructed as described previously [20]. 293T-pLV-ERα, 293T-pLV-ERβ or blank plasmid were co-transfected SKOV-3 cells with Lenti- Pac HIV Expression Packaging ...
11
Original Article Unfolded protein response mediated JNK/AP-1 signal transduction, a target for ovarian cancer treatment
... during hypoxia through autophagy [16]. However, further studies are needed to eluci- date the complex crosstalk between these pro- cesses and reveal their requirement in all stag- es of cancer development and ...
7
Carboplatin and oxaliplatin in sequenced combination with bortezomib in ovarian tumour models
... and SKOV-3 ovarian cancer cells with BORT alone and its combinations with CB and OX significantly reduced the total glutathione (GSH and GSSG) levels in all the three cell lines – more so from BORT alone ...
11
Resveratrol and acetyl-resveratrol modulate activity of VEGF and IL-8 in ovarian cancer cell aggregates via attenuation of the NF-κB protein
... the SKOV-3 cell ag- gregates is markedly ...the SKOV-3 aggregates suggesting that resveratrol may not be a single action compound, this is further supported by the opposing trends of IL-8 ...
12
MiR-137 and miR-34a directly target Snail and inhibit EMT, invasion and sphere-forming ability of ovarian cancer cells
... and SKOV-3 cells following trans- fection of miRNA mimics or inhibitors, ...into SKOV-3 cells (which has relatively higher levels of miR-34a and miR-137) up-regulated Snail expression at mRNA ...
9
Evaluation of the effects of hyaluronic acid-carboxymethyl cellulose barrier on ovarian tumor progression
... To determine the potential role of HA-CMC barrier in ovarian tumor growth, we established SKOV-3 cell s.c. xe- nografts in mice in both flanks. The day following this in- jection, we made an incision at the ...
12
Proton-sensing GPCR-YAP Signalling Promotes Cancer-associated Fibroblast Activation of Mesenchymal Stem Cells
... Next, we investigated whether interference of GPR68 can decrease CAF activation under cancer mi- croenvironment. We therefore co-cultured SKOV-3 cells with GPR68-silenced MSCs to determine wheth- er GPR68 ...
8
Cytotoxic effect of Alpinia scabra (Blume) Náves extracts on human breast and ovarian cancer cells
... Bioassay-guided fractionation of leaf chloroform extract The cytotoxic chloroform extract of leaves (9.0 g) was subjected to vacuum liquid chromatography (VLC) which is a rapid crude fractionation system. The column (7 ...
14
Transactivation of epidermal growth factor receptor through platelet-activating factor/receptor in ovarian cancer cells
... in SKOV-3 cells, HB-EGF production increased after 30 min of stimulation with PAF (100 nM) and continued to increase, reaching a maximum after 2 ...in SKOV-3 ...of SKOV-3 ...
12
Mitochondrially targeted p53 or DBD subdomain is superior to wild type p53 in ovarian cancer cells even with strong dominant negative mutant p53
... After confirming that our mitochondrial p53 constructs effectively localize to the mitochondria and do not have nuclear activity, we tested our constructs’ ability to in- duce cell death and apoptosis in ovarian cancer ...
17
Actively-targeted LTVSPWY peptide-modified magnetic nanoparticles for tumor imaging
... Results: The average diameter and zeta potential of the LTVSPWY-PEG-CS-modified magnetic nanoparticles was 267.3 ± 23.4 nm and 30.5 ± 7.0 mV, respectively, with a narrow size distribution and spherical morphology. In ...
9
H-RYK, an Unusual Receptor Kinase: Isolation and Analysis of Expression in Ovarian Cancer
... Material and Methods: An unusual receptor tyrosine kinase, H-RYK, has been isolated from a complimentary DNA library of SKOV-3, an epithelial ovarian cancer cell line, using a polymerase[r] ...
15
Formulation and evaluation of mixed polymeric micelles of quercetin for treatment of breast, ovarian, and multidrug resistant cancers
... lines was 33, 41, 11.1, and 71.2 µ M, respectively, while that of A22 was found to be 42, 24, 10.1, and 71 µ M respectively. Both the MPM formulations containing QCT exhibited enhanced cytotoxicity compared to pure QCT. ...
13
Malignant ascites enhances migratory and invasive properties of ovarian cancer cells with membrane bound IL-6R in vitro
... may affect tumor cells behavior [17]. To determine the cytokine(s) in ascites that are associated with EMT- dependent invasion of SKOV-3 cells, we evaluated a panel of cytokines using a cytokine array. ...
12
A novel far-red fluorescent xenograft model of ovarian carcinoma for preclinical evaluation of HER2-targeted immunotoxins
... line SKOV-kat belongs to a family of far-red fluorescent proteins that are well-suited for the in vivo imaging as their emission spectrum falls within the “window of tissue transparency” ...the SKOV-kat ...
10
The Role of Long Non-Coding RNAs in Ovarian Cancer
... HOTAIR is a long intervening non-coding RNA (lincRNA) transcribed from the HOXC locus, which participates in epigenetic regulatory processes, cooperates with polycomb repressive complex 2 and is required for histone H3 ...
13
The proliferation, apoptosis, invasion of endothelial-like epithelial ovarian cancer cells induced by hypoxia
... HUVEC, SKOV-3, SKOV-3 EL, ES-2, ES-2 EL, or the SKOV-3 or ES-2 cells treated by 50 nM Sirolimus) using TRIzol reagent (Invitrogen, Carlsbad, ...
8