Small interfering RNA transfection
Evaluation of Small Interfering RNA Delivery into Cells by Reverse Transfection in Suspension with Cationic Liposomes
... siRNA transfection method using cationic liposomes, we compared the transfection efficiency of siRNA between Fw-transfection and Rev-transfection methods with various types of cationic ...Fw- ...
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Adenovirus VA1 Noncoding RNA Can Inhibit Small Interfering RNA and MicroRNA Biogenesis
... VA1 RNA seen in HeLa cells infected with the E1A ⫺ E1B ⫺ adenovirus with the levels seen in pBS-VA1-transfected HeLa cells suggested that this low level of VA1 RNA expression explains the modest phenotype ...
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Multi-responsive microencapsulated nanogels for the oral delivery of small interfering RNA
... with RNA-PEI complexes to form a cationic core, which was then loaded with Endo-Porter, a peptide to facilitate intracellular delivery, followed by fluorescently-labeled siRNAs targeting TNF-α, and finally an ...
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Ebolavirus Proteins Suppress the Effects of Small Interfering RNA by Direct Interaction with the Mammalian RNA Interference Pathway
... Since assays for the measurement of viral protein are not routinely performed in biosafety level 4 (BSL4) containment, where this experiment was conducted, we instead evaluated viral load by plaque assay to assess the ...
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Inhibitory effect of small interfering RNA on dengue virus replication in mosquito cells
... Results: A 21 nt siRNA targeting the membrane glycoprotein precursor gene of DEN-1 was synthesized and transfected into mosquito C6/36 cells followed by challenge with DEN. The stability of the siRNA in cells was ...
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Role of the Trypanosoma brucei HEN1 Family Methyltransferase in Small Interfering RNA Modification
... to transfection of ␣ -tubulin ...total RNA was prepared 2 h after electroporation and analyzed by Northern hybridization with an ␣ -tubulin-specific probe ( ␣ -tubulin ...
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Efficient delivery of small interfering RNA for inhibition of IL-12p40 expression in vivo
... reversed (mutant siRNA), and siRNA targeting GFP, a pro- tein that does not naturally occur in J774A.1 cells. These control siRNAs did not induce IL-12p40 mRNA expres- sion (Fig. 1B). Our results show sequence-specific ...
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Inhibition of foot and mouth disease virus replication in vitro and in vivo by small interfering RNA
... cellular transfection and identifica- tion of FMDV were conducted in BHK-21 ...hairpin RNA is competent to trigger an antiviral response on BHK-21 ...
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Stable expression of small interfering RNA sensitizes TEL PDGFβR to inhibition with imatinib or rapamycin
... using RNA inter- ference ...stranded small interfering RNA (siRNA) in mammalian cells with- out initiating the nonspecific double-stranded RNA responses ...on transfection effi- ...
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Effective small interfering RNA delivery in vitro via a new stearylated cationic peptide
... the transfection efficiency of CPPs for DNA delivery 19–25 or that of polyethyleneimine for siRNA ...increased transfection efficiency is attributed to the enhanced compaction of DNA, increased endosomal ...
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Small-Interfering RNA-Eluting Surfaces as a Novel Concept for Intravascular Local Gene Silencing
... We found that different gelatin species used for the coating showed different E- selectin receptor knockdowns (data not shown). The use of gelatin that contains only short collagen fragments and has low gel strength ...
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RNA Interference against Animal Viruses: How Morbilliviruses Generate Extended Diversity To Escape Small Interfering RNA Control
... after transfection, cells were in- fected with 10-fold serial dilutions of the ...the transfection test, virus was harvested from the wells containing at least 25% CPE, pooled, freeze-thawed 3 times, and ...
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Deletion of Cytoplasmic Double-Stranded RNA Sensors Does Not Uncover Viral Small Interfering RNA Production in Human Cells
... The IFN and RNAi responses coexist in somatic cells; however, Maillard et al. suggested that a functional IFN pathway masks or inhibits the activity of RNAi (13). These authors showed that differentiated mouse embryonic ...
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Targets of Small Interfering RNA Restriction during Human Immunodeficiency Virus Type 1 Replication
... HIV-1 RNA regions rep- resenting different HIV-1 mRNA species: the RU5 region or CA-NC gag genes for genomic RNA, the env C1-C2 or env C2-V3 region for singly spliced as well as unspliced genomic ...after ...
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Alternative Approaches for Efficient Inhibition of Hepatitis C Virus RNA Replication by Small Interfering RNAs
... HCV RNA copy ...total RNA were determined by quantitative RT-PCR. Hatched columns, viral RNA concentration in control cells; black columns, viral RNA concentration after the ...
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Small interfering RNA against CD86 during allergen challenge blocks experimental allergic asthma
... low transfection efficacy might be a potential hurdle in RNA interference, it is expected that DCs can be good targets because of their location and their prominent particle uptake ability, including the ...
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Small interfering RNA targeting S100A4 sensitizes non-small-cell lung cancer cells (A549) to radiation treatment
... Results: Our results demonstrate that S100A4-siRNA effectively silenced the S100A4 gene. When siRNA against S100A4 was used, S100A4 protein expression was downregulated, whereas the expressions of E-cadherin and p53 were ...
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Sustained Small Interfering RNA-Mediated Human Immunodeficiency Virus Type 1 Inhibition in Primary Macrophages
... 2003 Small interfering RNAs (siRNAs) can induce potent gene silencing by degradation of cognate ...after transfection with CCR5 and p24 ...when transfection preceded infection by 15 days, ...
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Original Article Small interfering RNA-mediated inhibition of respiratory syncytial virus infections in Hela cells
... siRNAs Transfection of siRNAs into Hela cells was per- formed, according to manufacturer instruc- ...pre-prepared transfection com- pounds. Transfection compounds, formed by 30 μl DMEM medium, ...
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Small interfering RNA targeting NF-κB attenuates lipopolysaccharide-induced acute lung injury in rats
... of RNA interference (RNAi) lies in delivery and stable expression of siRNA and has been used to cure disorders of the eyes, lungs and cen- tral nervous systems [1, 2, 4, 20, ...of transfection reagents ...
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