small RNA (sRNA)
Altered Fecal Small RNA Profiles in Colorectal Cancer Reflect Gut Microbiome Composition in Stool Samples
... for small RNA sequencing (sRNA-Seq). sRNA transcripts were converted into barcoded cDNA ...multiplex small RNA library prep set for Illumina (protocol E7330; New England BioLabs ...
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Endurance training remodels sperm-borne small RNA expression and methylation at neurological gene hotspots
... Using small RNA (sRNA) sequencing and reduced representation bisulfite sequencing (RRBS), we, respectively, investigated sRNA expression and DNA methylation in pure fractions of motile ...
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Functionalization of an antisense small RNA
... antisense small RNA (sRNA) can trans-sequester a functional sRNA that targets an existing ...antisense sRNA may additionally acquire the ability to directly control gene expres- ...
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SePIA: RNA and small RNA sequence processing, integration, and analysis
... of RNA-seq protocols exist to produce total RNA, poly(A)-derived RNA and small RNA ...of RNA-seq protocol affects the design principles implemented in the computational analysis ...
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Isolation and detection of small RNA molecules
... detect small RNA molecules is extraction of all small RNA molecules from de- naturing polyacrylamide gel (“Crush and soak”; Sambrook and Russell 2001), cloning and se- ...of small ...
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The Heterocyst Specific NsiR1 Small RNA Is an Early Marker of Cell Differentiation in Cyanobacterial Filaments
... ABSTRACT Differentiation of single cells along filaments of cyanobacteria constitutes one of the simplest developmental patterns in nature. In response to nitrogen deficiency, certain cells located in a semiregular ...
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Emerging role of a novel small non-coding regulatory RNA: tRNA-derived small RNA
... of small non-coding RNAs, such as miRNA and piRNA, has dramatically changed our understanding of the role RNA plays in ...novel small non-coding RNA generated from cleavage of tRNA or ...
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A Central Small RNA Regulatory Circuit Controlling Bacterial Denitrification and N2O Emissions
... the sRNA-29-controlled regulatory ...between sRNA-29 and ...of sRNA-29 across the whole genome of P. denitrificans, we combined sRNA-29 overexpression with RNA sequencing ...with ...
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Changes in Aphid Host Plant Diet Influence the Small RNA Expression Profiles of Its Obligate Nutritional Symbiont, Buchnera
... Buchnera sRNA expression is more heterogenous among biological replicates in both life stages when ...in sRNA expression profiles, there is a significant difference in sRNA expression observed between ...
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The CpxQ sRNA Negatively Regulates Skp To Prevent Mistargeting of β Barrel Outer Membrane Proteins into the Cytoplasmic Membrane
... ABSTRACT The promoter most strongly induced upon activation of the Cpx two-component envelope stress response is the cpxP promoter. The 3= untranscribed region (UTR) of the cpxP transcript is shown to produce a ...
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A Unique cis Encoded Small Noncoding RNA Is Regulating Legionella pneumophila Hfq Expression in a Life Cycle Dependent Manner
... cis-encoded small RNA named Anti-hfq that is transcribed antisense of the hfq transcript and overlaps its 5= untranslated ...Anti-hfq sRNA is highly expressed only in replicating ...the small ...
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Genome-Wide Approaches To Study Rna Secondary Structure
... just RNA expression, but several other aspects of RNA ...of RNA biology: RNA-secondary structure, RNA cleavage, and regulatory small ...determining RNA secondary structure ...
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Reprogramming of Small Noncoding RNA Populations in Peripheral Blood Reveals Host Biomarkers for Latent and Active Mycobacterium tuberculosis Infection
... yielded small RNA biomarkers that can be used to identify difficult-to-diagnose TB cases regardless of level of lung injury (as seen on CXR images), bacterial load, or clinical ...for RNA amplifica- ...
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RNA-Based Immunity Terminates Viral Infection in Adult Drosophila in the Absence of Viral Suppression of RNA Interference: Characterization of Viral Small Interfering RNA Populations in Wild-Type and Mutant Flies
... virus-derived small RNA pools in WT and mutant fruit ...bars)-strand small RNAs showing perfect match with the two viral genomic RNAs in WT and loqs, dcr-2, and ago2 mutant flies inoculated with FHV ...
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sRNA Target Prediction Organizing Tool (SPOT) Integrates Computational and Experimental Data To Facilitate Functional Characterization of Bacterial Small RNAs
... identify sRNA candidate ...an sRNA. While microarrays and RNA sequencing have been successfully used to deduce sRNA targets, in many cases, separating direct effects from indirect effects is ...
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Small regulatory RNA induced growth rate heterogeneity of Bacillus subtilis
... traits. Small regulatory RNAs (sRNAs) could affect this heterogeneity since they act by fine- tuning mRNA or protein levels to coordinate the appropriate cellular ...the sRNA RnaC/S1022 from the ...
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More precise location of the polycytidylic acid tract in foot and mouth disease virus RNA.
... This treatment produces a small fragment S containing the small protein covalently linked to the RNA and a large fragment L that migrates faster than untreated RNA on low-percentage poly[r] ...
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Sorting nexin-4 regulates β-amyloid production by modulating β-site-activating cleavage enzyme-1
... Scrambled small interfering RNA; siRNA: Small interfering RNA; siSNX4: Small interfering RNA mixture; SNX: Sorting nexin; SNX4: Sorting nexin-4; SNX-BAR: SNX protein containing a ...
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A rhlI 5′ UTR Derived sRNA Regulates RhlR Dependent Quorum Sensing in Pseudomonas aeruginosa
... Disruption of the RhlS terminator reduces C4-HSL production. From the term- seq analysis, we estimate that RhlS is the predominant transcript derived from the rhII promoter, with full-length rhlI mRNA accounting for ...
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Naming 'junk': Human non-protein coding RNA (ncRNA) gene nomenclature
... termed splicing — in which introns are removed so that the exons can be joined to form a mature mRNA (for a review, see Ritchie et al. 17 ). Some introns are self-splicing but most require the inter- vention of the ...
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