Small subunit ribosomal DNA
Phylogenetic Analysis of Rhinosporidium seeberi’s 18S Small Subunit Ribosomal DNA Groups This Pathogen among Members of the Protoctistan Mesomycetozoa Clade
... For the past 100 years the phylogenetic affinities of Rhinosporidium seeberi have been controversial. Based on its morphological features, it has been classified as a protozoan or as a member of the kingdom Fungi. We ...
5
Cladistic relationships among the Pleurotus ostreatus complex, the Pleurotus pulmonarius complex, and Pleurotus eryngii based on the mitochondrial small subunit ribosomal DNA sequence analysis
... 12.5 pM primers and 10–50 ng of template total DNA. Amplification was carried out as follows: 1 cycle of 5 min at 94°C, followed by 30 cycles of 30 s at 94°C, 30 s at 60°C, and 90 s at 72°C, and finally, 1 cycle ...
6
Mitochondrial DNA Restriction Fragment Length Polymorphism (RFLP) and 18S Small Subunit Ribosomal DNA PCR RFLP Analyses of Acanthamoeba Isolated from Contact Lens Storage Cases of Residents in Southwestern Korea
... genomic DNA were extracted with equal volumes of phe- nol-chloroform by centrifugation at 12,000 rpm at 4°C for 5 ...precipitated DNA was washed with 70% chilled ethanol. The DNA sediment was vacuum ...
8
Alteration of a Novel Dispensable Mitochondrial Ribosomal Small-Subunit Protein, Rsm28p, Allows Translation of Defective COX2 mRNAs
... YDR494w/RSM28 sequence correction, disruption, and hemagglutinin tagging. YDR494w/RSM28 was PCR amplified from both NB64 and NB64-S7c to deter- mine the nature of the suppressor mutation. The sequences of the PCR ...
9
Pleurostomophora ochracea, a Novel Agent of Human Eumycetoma with Yellow Grains
... Genomic DNA was extracted by scraping material off Sabouraud plates, freezing it in liquid nitrogen, and grinding it with a mortar and ...pestle. DNA was extracted from the resulting pulp with the Promega ...
8
Ribosomal small subunit sequence diversity of Scutellospora within single spores and roots of bluebell from a woodland community.
... On the basis of our data, primers targeting variable regions of the SSU are unlikely to amplify DNA from all homologous genes present within a single spore. This has implications for the use of molecular methods ...
8
Natural infection of the sand fly Phlebotomus kazeruni by Trypanosoma species in Pakistan
... Genomic DNA was extracted from the ethanol-fixed specimen, and the cyto- chrome b (cyt b) gene was amplified from the flagellate with primers prepared for the leishmanial cyt b gene ...and small ...
5
LOCALIZATION IN THE TOMATO GENOME OF DNA RESTRICTION FRAGMENTS CONTAINING SEQUENCES HOMOLOGOUS TO THE rRNA (45S), THE MAJOR CHLOROPHYLL a/b BINDING POLYPEPTIDE AND THE RIBULOSE BISPHOSPHATE CARBOXYLASE GENES
... DNA restriction fragments containing sequences homologous to the ribosomal RNA (45s), the major chlorophyll a/b binding polypeptide (CAB) and the small subunit of ribulose bis[r] ...
13
The HIV 1 Nef Protein Interacts with two components of the 40S small ribosomal subunit, the RPS10 protein and the 18S rRNA
... of ribosomal RNA (rRNA), synthesis of ribosomal proteins and their import into the nucleus, the assembly of ribosome subunits, and the transport of the mature 40S and 60S subunits into the cytoplasm ...by ...
9
Hidden biodiversity revealed by integrated morphology and genetic species delimitation of spring dwelling water mite species (Acari, Parasitengona: Hydrachnidia)
... large subunit ribosomal RNA gene; 18S: small subunit ribosomal RNA gene; ABGD: Automatic Barcode Gap Discovery (species delimitation algorithm); BI: Bayesian inference (phylogenetic ...
13
Comparison of techniques for DNA extraction and agarose gel staining of DNA fragments using samples of Cryptosporidium
... two DNA extraction methods, several annealing temperatures for nested PCR reactions targeting the 18S, SSU rRNA (small subunit ribosomal RNA), and the GP60 (60 kDa glycoprotein) genes, and two ...
8
Epitope regions on U1 small nuclear RNA recognized by anti U1RNA specific autoantibodies
... Anti28S rRNA antibodies, present in sera from patients that also contain anti-P ribosomal protein antibody appear to recognize an epitope region in the 60S ribosomal subunit, which lies [r] ...
11
Phylogenetic Analysis of Lacazia loboiPlaces This Previously Uncharacterized Pathogen within the Dimorphic Onygenales
... revealed that with the exception of nucleotides at the extreme 5⬘ and 3⬘ ends, our sequence is identical to AF241655 and differs from AF227151 at three nucleotide positions in the middle of the molecule (two gaps and one ...
6
A complete mitochondrial genome sequence of Asian black bear Sichuan subspecies (Ursus thibetanus mupinensis)
... oxidase subunit 1, 2 and 3, ATP synthase F0 subunit 8 and ATP synthase F0 subunit 8), two rRNA genes (12s RNA and 16s RNA), and a full set of 22 ...
6
Host-Specific Replication of BK Virus DNA in Mouse Cell Extracts Is Independently Controlled by DNA Polymerase α-Primase and Inhibitory Activities
... single-stranded DNA (ssDNA) threading through the hexameric channels ...of DNA rep- lication (5, 7, 43). Then, DNA polymerase ␣ -primase (Pol- prim) is loaded onto this TAg-RPA-topoisomerase ...
9
The mitochondrial transcriptome of the anglerfish Lophius piscatorius
... mitochondrial small- and large-subunit ribosomal RNA; ND1–6, NADH dehydrogenase subunit 1 to 6; COI-III, cytochrome oxidase subunit I to III; A6 and A8, ATPase subunit 6 and 8; ...
7
An evolutionary study of two microsporidia
... The LSU of N. apis contains many features that identify it as eukaryotic. It is also comparatively very small when all three evolutionary lineages (eubacteria, archaebacteria and eukaryotes) are considered. This ...
196
Morad, Samy (2010): Pharmacological studies of a novel inhibitor of the mammalian target of rapamycin (mTOR) signaling pathway. Dissertation, LMU München: Tierärztliche Fakultät
... a small GTPase that belongs to a unique family within the Ras superfamily of ...The small GTPase Rheb is a positive upstream regulator of the target of ...
110
Experience with the MicroSeq D2 Large Subunit Ribosomal DNA Sequencing Kit for Identification of Filamentous Fungi Encountered in the Clinical Laboratory
... aerobic and anaerobic bacteria, mycobacteria, and fungi (in- cluding yeasts) (1, 2, 4, 6). Turenne et al. (6) showed that sequencing of the ITS2 region has great potential for the iden- tification of yeasts and several ...
5
Isolation And Identification Of Endophytic Fungi Rhizopus Delemar From Memecylon Umbellatum In Gudiyum Forest, Tamil Nadu, India
... on DNA sequences is recognized as the most reliable method to reveal genetic relationship between the strains which could be unambiguously used to identify and evaluate the isolates at any taxonomic ...
8