Somatic Cell Nuclear Transfer
Efficient generation of P53 biallelic knockout Diannan miniature pigs via TALENs and somatic cell nuclear transfer
... Somatic cell nuclear transfer (SCNT) was performed as previously described ...before nuclear transfer and then injected into the perivi- telline space of an enucleated ...donor ...
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Critical Developmental Stages for the Efficiency of Somatic Cell Nuclear Transfer in Zebrafish
... Somatic cell nuclear transfer (SCNT) has been performed extensively in fish since the 1960s with a generally low efficiency of approximately ...about somatic nuclear ...
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Interspecies somatic cell nuclear transfer is dependent on compatible mitochondrial DNA and reprogramming factors
... Interspecies somatic cell nuclear transfer (iSCNT) involves the transfer of a nucleus or cell from one species into the cytoplasm of an enucleated oocyte from ...the ...
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The influence of interspecies somatic cell nuclear transfer on epigenetic enzymes transcription in early embryos
... from somatic cell nuclear transfer is caused by insufficient demethylation of injected somatic chromatin to a state comparable with an early embryonic ...the nuclear ...
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The influence of donor nucleus source on the outcome of zebrafish somatic cell nuclear transfer
... of nuclear reprogramming following somatic cell nuclear transfer (SCNT) is thought to depend on factors present in the ...the somatic cell type on the outcome of the ...
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Generation of GHR modified pigs as Laron syndrome models via a dual sgRNAs/Cas9 system and somatic cell nuclear transfer
... GHRKO cell lines were established by single cell cloning ...knockout cell lines were selected as the donor cell line for somatic cell nuclear transfer for the ...
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Production of CFTR null and CFTR ΔF508 heterozygous pigs by adeno associated virus–mediated gene targeting and somatic cell nuclear transfer
... Progress toward understanding the pathogenesis of cystic fibrosis (CF) and developing effective therapies has been hampered by lack of a relevant animal model. CF mice fail to develop the lung and pancreatic disease that ...
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Efficient generation of GGTA1-null Diannan miniature pigs using TALENs combined with somatic cell nuclear transfer
... GTKO pigs were generated using traditional homolo- gous recombination (HR), zinc-finger nuclease (ZFN) gene editing technologies and somatic cell nuclear transfer (SCNT) methods [6–10]. ...
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Recombinant human activin A promotes development of bovine somatic cell nuclear transfer embryos matured in vitro
... bovine somatic cell nuclear transfer (SCNT) embryos, we studied the effects of activin A on developmental competence of bovine SCNT embryos during the early development stage based on the ...
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SV40 Large T Antigen Disrupts Embryogenesis of Canine and Porcine Somatic Cell Nuclear Transfer Embryo
... immortalized sheep fibroblast cells [26] and SV40LT- mediated transformed rabbit tracheal-epithelial cells, re- spectively [27]. However, we found that SCNT embryos derived from SV40LT-expressing canine and porcine ...
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Progress toward generating a ferret model of cystic fibrosis by somatic cell nuclear transfer
... 1000 cell clones. We settled on using a 96-well plate to screen crude cell lysates by ...of cell clones, and will thus likely increase cellular compe- tence for NT ...other cell type use- ful ...
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Generation of an MC3R knock-out pig by CRSPR/Cas9 combined with somatic cell nuclear transfer (SCNT) technology
... Currently, pigs are extensively used as a large-animal model for biomedical research because of their greater similarities with humans in terms of anatomy, physi- ology, and immunology, compared to rodents [11, 16, 17]. ...
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Fam60al as a novel factor involved in reprogramming of somatic cell nuclear transfer in zebrafish (Danio rerio)
... in somatic cell reprogramming of human pluripotent stem cells [25, ...in somatic cell reprogramming, which improved somatic cell induction efficiency by blocking the degradation ...
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Generation of bovine transgenics using somatic cell nuclear transfer
... cells somatic cells have a definitive ...transgenic cell line with a capacity for 30 PDs [17], Clarke and coworkers have estimated that gene targeting requires around 45 PDs ...between cell lines and ...
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Cloning animals by somatic cell nuclear transfer – biological factors
... of nuclear transfer ...the cell cycle stage of ...with nuclear transfer have uti- lized a serum starvation ...for nuclear transfer and four calves were born from 28 ...
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Somatic cell nuclear transfer: failures, successes and the challenges ahead
... As cell division begins, the total number of mitochondria within each blastomere decreases due to dilution with no new mitochondrial biosynthesis (John et ...the nuclear-encoded replication factors required ...
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Richter, Anne (2012): Genetic modification of primary cells for the generation of transgenic pig models using somatic cell nuclear transfer. Dissertation, LMU München: Tierärztliche Fakultät
... Similar cell quality was found in the control transfection without ...DNA transfer into the cells, because with lower voltage (100 V) successfully transfection was not ...
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Application of new biotechnologies for improvements in swine nutrition and pork production
... embryonic cell nuclear transfer ...called somatic cell nuclear transfer ...the transfer of a nucleus from a donor cell into a mature enucleated oocyte (an ...
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Development of genome engineering technologies in cattle: from random to specific
... embryo transfer into a recipient was performed because of the ease of the ...cattle, somatic cell nuclear transfer (SCNT) was introduced and successfully used to produce cloned ...
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Gene expression of OCT4, SOX2, KLF4 and MYC (OSKM) induced pluripotent stem cells: identification for potential mechanisms
... by cell fusion, somatic cell nuclear transfer and ectopic expression of defined factors including octamer binding transcription factor 4 (OCT4), SRY related high mobility group box ...
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