Southern blot
Parity among the randomly amplified polymorphic DNA method, multilocus enzyme electrophoresis, and Southern blot hybridization with the moderately repetitive DNA probe Ca3 for fingerprinting Candida albicans
... The RAPD method of DNA fingerprinting, in which genomic DNA is amplified with a single short primer with an arbitrary sequence, has also gained favor because it is less time-consuming than Southern blot ...
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RAPID SOUTHERN BLOT PROTOCOL FOR THE ANALYSIS OF TARGETED MUTAGENESIS IN THE MOUSE IGHG1 LOCUS
... The efficiency of homologous recombination relies on the lenght of the homology arms and the chromatin compactation of the locus. In ES cells the immuoglobulin locu is compacted. Thus, the homologous recombination events ...
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Analysis of human papillomavirus types in exophytic condylomata acuminata by hybrid capture and Southern blot techniques
... Exophytic condylomata acuminata of the external genitalia of 40 patients were analyzed for human papillomavirus HPV DNA by the Southern blot and hybrid capture methods.. All lesions were[r] ...
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Comparison of Southern blot hybridization and polymerase chain reaction methods for the detection of human papillomavirus DNA
... The larger number of samples that were HPV positive by PCR 2 compared with Southern blot hybridization was most evident among control women, because both methods detected HPV in the majo[r] ...
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Identification and characterization of Serpulina hyodysenteriae by restriction enzyme analysis and Southern blot analysis
... Chromosomal DNA restriction enzyme analysis and Southern blot hybridization were used to characterize Serpulina hyodysenteriae strains.. When chromosomal DNAs from selected strains refer[r] ...
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Application of nonradioactive southern blot foe detection of restriction fragment length polymorphism changes on chromosome 5 q21
... MATERIALS AND METHODS 21 2.1 Materials 21 2.2 Amplification of polymorphic probes by transformation into E Coli JM 83 2.3 Rapid miniisolation of transformants 2.4 Large scale purific[r] ...
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Repetitive DNA sequences as probes for Mycobacterium tuberculosis
... Since Southern blot hybridizations yield more specific information, the recombinants were hybridized with membranes containing BamHI digests ofDNA from members of the tuberculosis comple[r] ...
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A New Sensitive Method for Detection of Viroids
... This paper reports new protocol for detection of citrus viroids that has many advantages compared with conventional methods. This method designated RT-PCR-DBH is a combination of RT-PCR and dot-blot hybridization ...
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Comparative analysis of genetic variability among Borrelia burgdorferi isolates from Europe and the United States by restriction enzyme analysis, gene restriction fragment length polymorphism, and pulsed field gel electrophoresis
... A Fingerprint patterns obtained through Southern blot hybridization of the 83-kDa antigen gene to the chromosomal EcoRI digests of the tick isolates from central and eastern parts of the[r] ...
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Switching of Candida albicans during successive episodes of recurrent vaginitis
... In the present case study, we have employed newly developed Southern blot hybridization methods to assess strain relatedness and plating methods to assess the frequency of switching and [r] ...
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Sequence tags of provirus integration sites in DNAs of tumors induced by the murine retrovirus SL3-3.
... The number of provirus-flanking cellular coherent frag- ments obtained in a given tumor does not necessarily reflect the number of integrated proviruses in that tumor. As seen in Table 1, the ratio of the number of PCR ...
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Molecular cloning and physical mapping of the tupaia herpesvirus genome.
... Restriction maps for the restriction endonucleases EcoRI and Hindlll were constructed with data derived from Southern blot hybridizations of individual viral DNA fragments or cloned DNA [r] ...
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The Mitochondrial Nucleoid Protein, Mgm101p, of Saccharomyces cerevisiae Is Involved in the Maintenance of ρ+ and ori/rep-Devoid Petite Genomes but Is Not Required for Hypersuppressive ρ− mtDNA
... to Southern blot analysis, the hybrid- ization signals from the mgm101-1 mutants exposed to restrictive temperature were found mainly in a high- molecular-weight position as in a wild-type strain (not ...
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Reversible impairment of the ku80 gene by a recyclable marker in Aspergillus aculeatus
... Homologous recombination frequencies were deter- mined by simply replacing the AaargB gene with AnpyrG in pyrG13 and the A. aculeatus ku80 deletion-mutant (MR12). To construct the AaargB deletion cassette 1, the 5’ (981 ...
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Transfer and expression of the rabbit defensin NP-1 gene in lettuce (Lactuca sativa).
... by Southern blotting. In Southern blot hybridization experiments, DNA samples from the regenerated lettuces hybridized with the probe derived from the NP-1 gene (Figure 4A), and the intensities of ...
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CD3 negative lymphoproliferative disease of granular lymphocytes containing Epstein Barr viral DNA
... Southern blot analysis of DNA obtained from the peripheral blood of seven patients with LDGL, including one with CAEBV infection. Interestingly, EBV DNA was detected in the sample from the patient with ...
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Geno and phenotypic characteristics of a transfected Babesia bovis 6 Cys E knockout clonal line
... the Southern blot analysis indicated that: (i) both the mixed transfected and clonal strain, but not the wild-type non-transfected, parasites contain a single copy of the egfp-bsd gene; (ii) the clonal ...
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restriction-enzyme-lab-b.pdf
... produces tens or hundreds of thousands of DNA fragments. When run on an agarose gel, the sample will appear as a long smear with no resolution visible due to the extremely high number of fragments of all sizes. One way ...
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Modulation of Antioxidant Defense in Aspergillus parasiticus Is Involved in Aflatoxin Biosynthesis: a Role for the ApyapA Gene
... (i) Southern blot hybridization with probe ...after Southern analysis of the fungal DNA digested with EcoRI (which does not restrict ApyapA but cuts the amdS sequence once at ⫹4361 and the Hph coding ...
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Ribosomal DNA is a site of chromosome breakage in aneuploid strains of Neurospora.
... normal sequence derivatives o f a partial diploid. The Southern blot was probed with ra- diolabeled rDNA. cereuisiae chromosomes were used as size standards. 3), then [r] ...
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