Southern Blot Hybridization
Comparison of Southern blot hybridization and polymerase chain reaction methods for the detection of human papillomavirus DNA
... The larger number of samples that were HPV positive by PCR 2 compared with Southern blot hybridization was most evident among control women, because both methods detected HPV in the majo[r] ...
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Application of different chromosomal DNA restriction digest fingerprints to specific and subspecific identification of Campylobacter isolates
... Strains were compared by analyses of HaeIII and XhoI digest patterns of chromosomal DNA and Southern blot hybridization patterns of XhoI digests obtained with an Escherichia coli 16S + 2[r] ...
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A general primer GP5+/GP6(+) mediated PCR enzyme immunoassay method for rapid detection of 14 high risk and 6 low risk human papillomavirus genotypes in cervical scrapings
... radioactive Southern blot hybridization using a general probe on the same PCR products derived from 417 cytomorphologically abnormal cervical scrapings resulted in an overall agreement of 96% between ...
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Identification and characterization of Serpulina hyodysenteriae by restriction enzyme analysis and Southern blot analysis
... Chromosomal DNA restriction enzyme analysis and Southern blot hybridization were used to characterize Serpulina hyodysenteriae strains.. When chromosomal DNAs from selected strains refer[r] ...
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A New Sensitive Method for Detection of Viroids
... We examined whether viroids could be detected from their natural host tissues by dot- blot hybridization RT-PCR, southern blot and RT-PCR-DBH. We then compared the sensitivity of these four ...
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Targeted Hybridization of IS6110 Fingerprints Identifies the W Beijing Mycobacterium tuberculosis Strains among Clinical Isolates
... strains with multiple (15 to 26) copies of IS6110 that share similar restriction fragment length polymorphism (RFLP) genotyping patterns ( ⬎ 65%), and that have at least one spe- cific IS6110 insertion within the NTF ...
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Inverted repeat regions of Marek's disease virus DNA possess a structure similar to that of the a sequence of herpes simplex virus DNA and contain host cell telomere sequences.
... To determine the number of a-like sequences within the termini and the L-S junction region, we performed Southern blot hybridization using BamHI-BalI-digested genomic DNA and the GGGTTA5[r] ...
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Comparative analysis of genetic variability among Borrelia burgdorferi isolates from Europe and the United States by restriction enzyme analysis, gene restriction fragment length polymorphism, and pulsed field gel electrophoresis
... A Fingerprint patterns obtained through Southern blot hybridization of the 83-kDa antigen gene to the chromosomal EcoRI digests of the tick isolates from central and eastern parts of the[r] ...
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Switching of Candida albicans during successive episodes of recurrent vaginitis
... In the present case study, we have employed newly developed Southern blot hybridization methods to assess strain relatedness and plating methods to assess the frequency of switching and [r] ...
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Involvement of human papillomavirus type 8 genes E6 and E7 in transformation and replication.
... After transfection of C127 cells, 14 G418-resistant lines were established and the presence of the E6 mutant construct was confirmed by Southern blot hybridization Downloaded from http:/[r] ...
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quemao, a Drosophila Bristle Locus, Encodes Geranylgeranyl Pyrophosphate Synthase
... was used to screen the cDNA library, which led to identification of the SRP19 transcript, and the 5.5 S/S fragment was used for Southern blot hybridization (see Figure 4), whereas the 5.5 O/O ...
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Development and Characterization of Complex DNA Fingerprinting Probes for the Infectious Yeast Candida dubliniensis
... Using a strategy to clone large genomic sequences containing repetitive elements from the infectious yeast Candida dubliniensis, the three unrelated sequences Cd1, Cd24, and Cd25, with respective molecular sizes of ...
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Modulation of Antioxidant Defense in Aspergillus parasiticus Is Involved in Aflatoxin Biosynthesis: a Role for the ApyapA Gene
... (i) Southern blot hybridization with probe ...after Southern analysis of the fungal DNA digested with EcoRI (which does not restrict ApyapA but cuts the amdS sequence once at ⫹4361 and the Hph ...
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Transfer and expression of the rabbit defensin NP-1 gene in lettuce (Lactuca sativa).
... with Southern blot hybridization signals were detected by western blotting, which showed hybridization signals in the Southern ...
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Emergence of a new clone of toxigenic Vibrio cholerae O1 biotype El Tor displacing V cholerae O139 Bengal in Bangladesh
... and Southern blot hybridization of the toxR gene showed that both of these regulatory genes are highly con- served among the 82 El Tor strains ...
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Detection of Theileria annulata in blood samples of carrier cattle by PCR
... Test samples. Experimental calves (Bos taurus) approximately 1 year of age were maintained in experimental tick-proof pens. The animals were experimen- tally infected with one of the four different stocks of T. annulata ...
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Outbreak of Dysentery Associated with Ceftriaxone Resistant Shigella sonnei: First Report of Plasmid Mediated CMY 2 Type AmpC β Lactamase Resistance in S sonnei
... Shigella spp. have managed to survive the antibiotic era via an ingenious mechanism of resistance, the production of  -lac- tamases. High rates of resistance to ampicillin among Shigella isolates are due to the ...
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Identification of Mycobacterium kansasii by DNA hybridization
... Briefly, this procedure involves digestion of chromosomal DNA with restriction enzymes and Southern blot hybridization by using plasmid pKK3535 as the probe.. This plasmid contains the r[r] ...
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Rapid Detection of Chlamydia pneumoniae by PCR Enzyme Immunoassay
... Since Southern analysis is too labor- intensive for routine use in diagnostic laboratories, an EIA for detection of PCR products was ...and Southern blot hybridization and demonstrated the ...
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ts1-Induced spongiform encephalomyelopathy: physical forms of high-mobility DNA in spinal cord tissues of paralyzed mice are products of premature termination of reverse transcription.
... by Southern blot hybridization with additional ecotropic virus-specific probes that hybridized to the p15E-coding region of the env gene and to two locations in the U3 region of the long terminal ...
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