Southern Blotting
Evaluation of Human Adipocyte Stem Cells Telomere Length in Early and Late Passages by Quantitative PCR and Southern Blotting
... For telomere length evaluation by Southern blotting, steps were taken based on previous study [18] with a slight modification. Briefly, about 10 µg of the same DNA that was used for qPCR, was double cut by ...
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Analysis of tissue-specific methylation patterns of mouse mammary tumor virus DNA by two-dimensional Southern blotting.
... We used this technique with the MuMTV proviruses in the GR/A mouse strain to establish that in tissue displaying MuMTV proviral hypomethylation i different proviruses are hypomethylated [r] ...
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Several groups among human herpesvirus 6 strains can be distinguished by Southern blotting and polymerase chain reaction
... Hence, despite this additional subdivision among group II strains, the genetic polymorphism of the amplified 830-bp fragment correlated with the variability observed in the Southern blot[r] ...
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Microplate hybridization for Borna disease virus RNA in human peripheral blood mononuclear cells.
... ern blotting and microplate hybridization with FL-labeled probes ...by Southern blotting ...by Southern blotting and microplate hybridization with FL-labeled ...then ...
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Lys-34, Dispensable for Integrase Catalysis, Is Required for Preintegration Complex Function and Human Immunodeficiency Virus Type 1 Replication
... by Southern blotting can have its limitations, and because of this, we developed a novel PCR-based assay to quantify levels of HIV-1 PIC activity in in vitro integration ...Whereas Southern ...
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The c-myc Locus Is a Common Integration Site in Type B Retrovirus-Induced T-Cell Lymphomas
... (A) Southern blot analysis of TBLV-induced ...to Southern blotting and hybridization with the pSVcmyc1 probe (19); this probe contains genomic DNA spanning c-myc exons 2 and ...extractions, ...
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TbKAP6, a Mitochondrial HMG Box-Containing Protein in Trypanosoma brucei, Is the First Trypanosomatid Kinetoplast-Associated Protein Essential for Kinetoplast DNA Replication and Maintenance
... conducted Southern blotting of HindIII/XbaI-digested total DNA (these enzymes cleave all minicircles and maxicircles) isolated from uninduced cells or from three independent clonal TbKAP6-depleted cell ...
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Protein covalently bound to minus-strand DNA intermediates of duck hepatitis B virus.
... MASON Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111 Analysis of duck hepatitis B viral DNA by gel electrophoresis, Southern blotting, and bindi[r] ...
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Extrachromosomal Recombinant Adeno-Associated Virus Vector Genomes Are Primarily Responsible for Stable Liver Transduction In Vivo
... particles of AAV-EF1␣-F.IX (Fig. 1) via the portal vein (Table 1, group 1). Animals were sacrificed at 3, 10, and 19 months postinjection (n ⫽ 1, 1, and 2, respectively) and analyzed by Southern blotting. ...
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Synthesis of plus- and minus-strand RNA from poliovirion RNA template in vitro.
... The polarity of the products synthesized from plus-strand poliovirus RNA template was analyzed by Southern blotting using labeled product RNA to probe single-stranded poliovirus DNAs clo[r] ...
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De novo DNA methylation through the 5′ segment of the H19 ICR maintains its imprint during early embryogenesis
... Fig. 6. DNA methylation status of the 5 ′ -deleted endogenous H19 ICR. (A) Map of the wild-type and mutant H19 gene loci. The H19 ICR 5 ′ -region (765 bp, shaded area) was excised from the endogenous locus (mutant allele ...
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When Epstein-Barr virus persistently infects B-cell lines, it frequently integrates.
... 1245 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest In this study we used Gardella gel analysis of intact DNA, Southern blotting of digested DNA, and fluorescence in s[r] ...
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Analysis of junction sequences resulting from integration at nonhomologous loci in Neurospora crassa.
... rearranged vector sequences to preexisting breaks in chromosomal DNA. This fits well with more extensive data that we have obtained by genomic Southern blotting of DNAs [r] ...
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Phosphoacceptors Threonine 162 and Serines 170 and 178 within the Carboxyl-Terminal RRRS/T Motif of the Hepatitis B Virus Core Protein Make Multiple Contributions to Hepatitis B Virus Replication
... acid blotting from core particles containing STSSSS (WT) or mutant C ...acid blotting. After core particle Western blotting, the PVDF membrane was treated with ...for Southern blotting ...
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Transforming Sloan-Kettering viruses generated from the cloned v-ski oncogene by in vitro and in vivo recombinations.
... Having discovered that the 8.9-kb SKV genome and its product p125 were present in the mass-infected CECs we analyzed by Southern blotting, we reexamined the Southern blot of genomic DNA [r] ...
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Genetic and antigenic characterization of Borrelia coriaceae, putative agent of epizootic bovine abortion
... Borrelia coriaceae was characterized genetically and antigenically by utilizing the following techniques: restriction endonuclease analysis, Southern blotting and genomic hybridization, [r] ...
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Improved diagnosis of Duchenne/Becker muscular dystrophy
... The new molecular tests for DMD/BMD fall into two groups: those that examine dystrophin protein western blotting and immunofluorescence and those that study the gene Southern blotting wi[r] ...
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Transfer of 15 lipoxygenase gene into rabbit iliac arteries results in the appearance of oxidation specific lipid protein adducts characteristic of oxidized low density lipoprotein
... 15-LO-transduced cells contained human 15-LO mRNA and immunoreactive protein, as analyzed by Northern blotting and Western blotting, respectively, whereas no 15-LO mRNA or protein was de[r] ...
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<p>Long Non-Coding RNA SNHG14 Contributes to the Development of Hepatocellular Carcinoma via Sponging miR-217</p>
... Low expression of miR-217 was associated with vas- cular invasion, advanced TNM stage and poor prognosis in patients with HCC. 22 miR-217 was then discovered as a decreased miRNA in HCC cells with strong invasive ...
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Original Article KRT14 promoting invasion and migration of lung cancer cells through ROCK-1 signaling pathway
... An appropriate amount of cell lysis buffer con- taining protease inhibitor was added into the A549 cells, lysed for 30 min on ice and centri- fuged for 30 min at maximum rotation rate to obtain supernatant. Added with 1 ...
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