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Southern hybridization

Human papillomavirus type 18 E6 and E7 antibodies in human sera: increased anti E7 prevalence in cervical cancer patients

Human papillomavirus type 18 E6 and E7 antibodies in human sera: increased anti E7 prevalence in cervical cancer patients

... The type specificity of anti-HPV-18 E7 antibodies was demonstrated when the HPV type found by Southern hybridization in the cervical cancer biopsies was compared with seroreactivity: 4 o[r] ...

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SIRE-1, A putative plant retrovirus is closely related to a legume TY1-copia retrotransposon family

SIRE-1, A putative plant retrovirus is closely related to a legume TY1-copia retrotransposon family

... and Southern hybridization data show that soybean SIRE-1 sequences form a single envelope-containing lineage within a closely related and widespread population of envelope-lacking legume Ty1-copia ...

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Characterization of enteroinvasive Escherichia coli strains isolated from children with diarrhea in Chile

Characterization of enteroinvasive Escherichia coli strains isolated from children with diarrhea in Chile

... Bioassays and colony and Southern hybridization tests with iron uptake DNA probes indicated that 80% of the EIEC strains produced aerobactin and expressed its receptor, the genes for whi[r] ...

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Evolutionarily new sequences expressed in tumors

Evolutionarily new sequences expressed in tumors

... human. Southern hybridization analysis reveals only homology sequences in chicken genome for AA166653- specific ...by Southern hybridization that a sequence homologous to the human ...

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Temperate bacteriophages of Streptococcus pneumoniae that contain protein covalently linked to the 5' ends of their DNA.

Temperate bacteriophages of Streptococcus pneumoniae that contain protein covalently linked to the 5' ends of their DNA.

... Comparative pulsed-field gel electrophoresis analysis and Southern hybridization of the SmaI restriction fragments of DNAs from one lysogenic bacteria and its parental strain revealed th[r] ...

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Further characterization of Dothistromin genes in the fungal forest pathogen Dothistroma septosporum : a thesis presented in partial fulfilment of the requirements for the degree of Masters of Science in Molecular Genetics at Massey University, Palmerston

Further characterization of Dothistromin genes in the fungal forest pathogen Dothistroma septosporum : a thesis presented in partial fulfilment of the requirements for the degree of Masters of Science in Molecular Genetics at Massey University, Palmerston North, New Zealand

... SEPTOSPORUM 3.3 IDENTIFICATION OF THE PKSA GENE DISRUPTION MUTANTS 3.3.1 PCR to screen the transformants 3.3.2 Purification of the pksA mutants 3.3.3 Southern hybridization to confirm th[r] ...

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Further characterization of Dothistromin genes in the fungal forest pathogen Dothistroma septosporum : a thesis presented in partial fulfilment of the requirements for the degree of Masters of Science in Molecular Genetics at Massey University, Palmerston

Further characterization of Dothistromin genes in the fungal forest pathogen Dothistroma septosporum : a thesis presented in partial fulfilment of the requirements for the degree of Masters of Science in Molecular Genetics at Massey University, Palmerston North, New Zealand

... SEPTOSPORUM 3.3 IDENTIFICATION OF THE PKSA GENE DISRUPTION MUTANTS 3.3.1 PCR to screen the transformants 3.3.2 Purification of the pksA mutants 3.3.3 Southern hybridization to confirm th[r] ...

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Reversible changes in the composition of the population of mtDNAs during dedifferentiation and regeneration in tobacco.

Reversible changes in the composition of the population of mtDNAs during dedifferentiation and regeneration in tobacco.

... Reversible changes in mtDNA population: We screened for changes in the mtDNA population by com- paring the Southern hybridization profiles of total DNA from green plants and [r] ...

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Novel Non-Mendelian Determinant Involved in the Control of Translation Accuracy in Saccharomyces cerevisiae

Novel Non-Mendelian Determinant Involved in the Control of Translation Accuracy in Saccharomyces cerevisiae

... Southern hybridization of chromosomal DNA with the labeled cause elimination of [ PSI ⫹ ] ( Chernoff et al. The strain ⌬ HSP104-25-2V-P3982 contained the Partial inactivation of Sup35p e[r] ...

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Molecular evidence of person to person transmission of a pigmented strain of Corynebacterium striatum in intensive care units

Molecular evidence of person to person transmission of a pigmented strain of Corynebacterium striatum in intensive care units

... DNA restriction fragment patterns and Southern hybridization with the att site probe of Corynebacterium diphtheriae indicated that the brown isolates were a single strain which was disti[r] ...

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Characterization of a Mycobacterium tuberculosis insertion sequence, IS6110, and its application in diagnosis

Characterization of a Mycobacterium tuberculosis insertion sequence, IS6110, and its application in diagnosis

... Southern hybridization analysis of total mycobacterial DNAs probed with an IS6110 internal fragment, the 902-bp fragment from pMT02, as the probe.. scrofulaceum lane 7, and M.[r] ...

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Phage phi C2 Mediates Transduction of Tn6215, Encoding Erythromycin Resistance, between Clostridium difficile Strains

Phage phi C2 Mediates Transduction of Tn6215, Encoding Erythromycin Resistance, between Clostridium difficile Strains

... donor. Southern hybridization with the RF amplicon probe ...S1). Southern hybridization with a probe specific only for the right end of Tn6215 ...

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Sex Pheromone Response, Clumping, and Slime Production in Enterococcal Strains Isolated from Occluded Biliary Stents

Sex Pheromone Response, Clumping, and Slime Production in Enterococcal Strains Isolated from Occluded Biliary Stents

... Plasmid analysis, PFGE, and Southern hybridization. Plasmid DNA was extracted as previously described (3). The presence of plasmid DNA was mon- itored by 0.8% agarose gel electrophoresis. Genomic DNA for ...

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Identification of meningococcal serosubtypes by polymerase chain reaction

Identification of meningococcal serosubtypes by polymerase chain reaction

... Southern hybridization experiments demonstrated that it was possible to identify genes encoding different serological variants of the meningococcal class 1 outer membrane protein by prob[r] ...

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Single and Double Infections with Wolbachia in the Parasitic Wasp Nasonia vitripennis Effects on Compatibility

Single and Double Infections with Wolbachia in the Parasitic Wasp Nasonia vitripennis Effects on Compatibility

... uitripennis by Southern hybridization as well as PCR amplification, investigate the effects of diapause on bac- terial infection level and the production of single infec- tions[r] ...

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Detection of latency-associated transcripts of equid herpesvirus 1 in equine leukocytes but not in trigeminal ganglia.

Detection of latency-associated transcripts of equid herpesvirus 1 in equine leukocytes but not in trigeminal ganglia.

... Identification of LATs by random RT-PCR and Southern hybridization. Initially, random RT-PCR was carried out using RNA obtained from PBLs from two ponies and BLNs from three other ponies, all of which had ...

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Sulfolobus tengchongensis Spindle-Shaped Virus STSV1: Virus-Host Interactions and Genomic Features

Sulfolobus tengchongensis Spindle-Shaped Virus STSV1: Virus-Host Interactions and Genomic Features

... a Southern hy- bridization analysis on DNAs from both STSV1 and RT8-4 cells infected with the virus using either the entire viral genome sequence or the putative int gene as a ...No hybridization bands that ...

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Modulation of Antioxidant Defense in Aspergillus parasiticus Is Involved in Aflatoxin Biosynthesis: a Role for the ApyapA Gene

Modulation of Antioxidant Defense in Aspergillus parasiticus Is Involved in Aflatoxin Biosynthesis: a Role for the ApyapA Gene

... (i) Southern blot hybridization with probe ...after Southern analysis of the fungal DNA digested with EcoRI (which does not restrict ApyapA but cuts the amdS sequence once at ⫹4361 and the Hph coding ...

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Variation and Hybridization in Southern California Populations of Diplacus (Scrophulariaceae)

Variation and Hybridization in Southern California Populations of Diplacus (Scrophulariaceae)

... munities of southern California.. 6.-Southern California distribution of five Diplacus species; D. clevelandii, distribu- tion in southern part of Cuyamaca and Laguna M[r] ...

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Involvement of human papillomavirus type 8 genes E6 and E7 in transformation and replication.

Involvement of human papillomavirus type 8 genes E6 and E7 in transformation and replication.

... After transfection of C127 cells, 14 G418-resistant lines were established and the presence of the E6 mutant construct was confirmed by Southern blot hybridization Downloaded from http:/[r] ...

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