sphingosine kinase 1
Sphingosine kinase 1 as an anticancer therapeutic target
... Abstract: The development of chemotherapeutic resistance is a major challenge in oncology. Elevated sphingosine kinase 1 (SK1) levels is predictive of a poor prognosis, and SK1 overexpression may ...
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Regulation of cellular sphingosine-1-phosphate by sphingosine kinase 1 and sphingosine-1-phopshate lyase determines chemotherapy resistance in gastroesophageal cancer
... increased sphingosine kinase 1(SPHK1) mRNA and also with decreased sphingosine-1-phosphate lysase 1(SGPL1) ...cellular sphingosine-1-phosphate (S1P), and S1P ...
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Sphingosine kinase 1 and sphingosine 1 phosphate receptor 2 are vital to recovery from anaphylactic shock in mice
... Sphingosine kinase 1 (SphK1) and SphK2 are ubiquitous enzymes that generate sphingosine-1-phosphate (S1P), a ligand for a family of G protein–coupled receptors (S1PR1–S1PR5) with ...
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Sphingosine kinase 1 enables communication between melanoma cells and fibroblasts that provides a new link to metastasis
... Sphingosine 1-phosphate (S1P) is a bioactive lipid that has been demonstrated to have an important role in cancer including the promotion of metastasis of tumour cells 1 ...host sphingosine ...
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Expressions of Sphingosine 1 phosphate (S1P) Receptors, Sphingosine Kinases in Malignant Bone and Soft Tissue Tumors, and The role of Sphingosine Kinase 1 in Growth of MFH Cell Lines
... ceramide, sphingosine, and sphingos- ine-1-phosphate (S1P) have important physiological functions, including regulation of cell growth and ...of sphingosine catalyzed by sphingosine ...
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Leptin induces upregulation of sphingosine kinase 1 in oestrogen receptor negative breast cancer via Src family kinase mediated, janus kinase 2 independent pathway
... kinases 1/2; FCS: foetal calf serum; FFPE: formalin-fixed and paraffin- embedded; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; GUSB: beta glucuronidase; JAK2: janus kinase 2; LEPR: leptin receptor; ...
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Expression of sphingosine kinase 1 in amoeboid microglial cells in the corpus callosum of postnatal rats
... Sphingosine kinase 1 (SphK1), a key enzyme responsible for phosphorylating sphingosine into sphingosine-1- phosphate (S1P) has been shown to be expressed in monocytes and ...
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Original Article Resveratrol induces apoptosis of leukemia cell line K562 by modulation of sphingosine kinase-1 pathway
... S1P and ceramide are closely involved in regu- lating cell responses such as proliferation and apoptosis, the balance between ceramide and S1P is important to determine faith of cancer cells to undergo growth inhibition ...
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Leucine-rich Repeats and Immunoglobulin 1 (LRIG1) Ameliorates Liver Fibrosis and Hepatic Stellate Cell Activation via Inhibiting Sphingosine Kinase 1 (SphK1)/Sphingosine-1-Phosphate (S1P) Pathway
... immunoglobulin 1 (LRIG1) overexpression on liver fibrosis and sphingosine kinase 1 (SphK1)/Sphingosine-1-Phosphate (S1P) pathway activation in mice induced by carbon ...
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An novel role of sphingosine kinase 1 (SPHK1) in the invasion and metastasis of esophageal carcinoma
... lipid kinase sphingosine kinase-1 ...of sphingosine 1-phosphate (S1P), by which E2 is capable of activating the S1P receptor Edg-3, resulting in the EGFR transactivation in a ...
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Sphingosine kinase 1 is a potential therapeutic target for nasopharyngeal carcinoma
... Sphingosine kinase 1 (SPHK1) has been shown to be involved in the progression of various types of human cancers. We previously demonstrated that SPHK1 is overexpressed and associated with clinical ...
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Proteasomal degradation of sphingosine kinase 1 and inhibition of dihydroceramide desaturase by the sphingosine kinase inhibitors, SKi or ABC294640, induces growth arrest in androgen-independent LNCaP-AI prostate cancer cells
... Upon treatment, LNCaP-AI cells were lysed in sample buffer (62.5 mM Tris-HCl (pH 6.7), 0.5 M sodium pyrophosphate, 1.25 mM EDTA, 1.25% (w/v) sodium dodecyl sulphate, 0.06% (w/v) bromophenol blue, 12.5% (v/v) glycerol ...
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Proteasomal degradation of sphingosine kinase 1 and inhibition of dihydroceramide desaturase by the sphingosine kinase inhibitors, SKi or ABC294640, induces growth arrest in androgen-independent LNCaP-AI prostate cancer cells
... We have previously demonstrated that SKi induces the proteasomal degradation of SK1a and SK1b (which has an 86 amino-acid N-terminal extension compared with SK1a) in androgen-sensitive LNCaP prostate cancer cells and ...
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The roles of sphingosine kinase 1 and 2 in regulating the metabolome and survival of prostate cancer cells
... LNCaP prostate cancer cells express two N-terminal variant isoforms of SK1: SK1a (GenBank number: NM_001142601), which is a 42.5-kDa protein, and SK1b (GenBank number: NM_182965), which is a 51-kDa protein identical to ...
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Sphingosine kinase 1 is a reliable prognostic factor and a novel therapeutic target for uterine cervical cancer
... the sphingosine kinase (SPHK) family have received the most attention as important enzymes in cancer biology because their catalytic activity lies at a critical intersection in the regulation of bioactive ...
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Requirement for sphingosine kinase 1 in mediating phase 1 of the hypotensive response to anandamide in the anaesthetised mouse
... In order to determine which SK isoform was important in mediating the AEA-induced hypotension, these experiments were repeated using SK1- and SK2-selective inhibitors, BML-258 (SK1-I) and ROMe, re- spectively. Neither ...
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Everolimus (RAD001) sensitizes prostate cancer cells to docetaxel by down-regulation of HIF-1α and sphingosine kinase 1
... reduced both p-P70S6K and HIF-1α levels both alone and in combination with docetaxel (Figure 2A; Supplementary Figure S2A). We have also used an ELISA assay to analyze P70S6K phosphorylation, which showed similar results ...
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Downregulation of sphingosine kinase-1 induces protective tumor immunity by promoting M1 macrophage response in melanoma
... Transmembrane cell migration assays were performed using Boyden chambers containing membranes with a pore size of 8 µm (Corning). One million cells of BMDM or THP-1 cells were suspended in serum- free DMEM in the ...
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Sphingosine kinase 1 mediates head & neck squamous cell carcinoma invasion through sphingosine 1-phosphate receptor 1
... cells were seeded in a 6-well plate in 1 ml of media for 24 hours. Media was collected, centrifuged and pure supernatant was analyzed. Media was diluted 1:5 prior to assay and IL-6 concentration was ...
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Sphingosine kinase 1 expression enhances colon tumor growth
... vector was constructed according to the previous study [14]. HT-29 cells were transfected with hSphK1-GFP vec- tor or GFP empty vector using Lipofectamine 2000 (Inv- itrogen) according to the manufacturer’s protocol. ...
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