sphingosine kinase 1/2
Leptin induces upregulation of sphingosine kinase 1 in oestrogen receptor negative breast cancer via Src family kinase mediated, janus kinase 2 independent pathway
... kinases 1/2; FCS: foetal calf serum; FFPE: formalin-fixed and paraffin- embedded; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; GUSB: beta glucuronidase; JAK2: janus kinase 2; LEPR: leptin ...
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The roles of sphingosine kinase 1 and 2 in regulating the metabolome and survival of prostate cancer cells
... We have previously shown that (S)-FTY720 vinylphosphonate induces apoptosis as assessed by the increased cleavage of polyADPribose polymerase (PARP), which is a DNA repair enzyme, in LNCaP- AI cells [8]. We now show that ...
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Sphingosine kinase 1 and sphingosine 1 phosphate receptor 2 are vital to recovery from anaphylactic shock in mice
... marrow cells (n = 6). **P < 0.01, ***P < 0.001, determined using 2-way ANOVA. Blood analysis in all the lethally-irradiated, bone marrow–transplanted mice showed cell counts similar to those of WT mice, ...
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Crystal structure of sphingosine kinase 1 with PF-543
... of sphingosine to sphingosine-1-phosphate (S1P), the fi nal step of S1P ...biosynthesis. 1−4 There are two sphingosine kinases: Sphingo- sine Kinase 1 (SPHK1) is mostly ...
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Sphingosine kinase 1 mediates head & neck squamous cell carcinoma invasion through sphingosine 1-phosphate receptor 1
... An in vitro invasion assay was performed using a 24-well format in quadruplicate according to manufac- turer’s guidelines (BD BioCoat Matrigel Invasion chamber, BD Biosciences, San Jose, CA). Briefly, after hydration of ...
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Requirement for sphingosine kinase 1 in mediating phase 1 of the hypotensive response to anandamide in the anaesthetised mouse
... In order to determine which SK isoform was important in mediating the AEA-induced hypotension, these experiments were repeated using SK1- and SK2-selective inhibitors, BML-258 (SK1-I) and ROMe, re- spectively. Neither ...
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Reduced sphingosine kinase-1 and enhanced sphingosine 1-phosphate lyase expression demonstrate deregulated sphingosine 1-phosphate signaling in Alzheimer’s disease
... Human brain tissues were provided by certified French biological resource centers from Lille (DC-2008-642), Paris (DC-2009-957) and Toulouse (AC-2009-973), and by the national brain bank GIE Neuro-CEB (AC-2007-5). This ...
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Regulation of cellular sphingosine-1-phosphate by sphingosine kinase 1 and sphingosine-1-phopshate lyase determines chemotherapy resistance in gastroesophageal cancer
... with complete media alone (no cells) as a background control, and blank and vehicle controls included on each plate. Unless otherwise stated, all measurements were performed in triplicate independent experiments with ...
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Sphingosine kinase 1 expression enhances colon tumor growth
... (AP7237c; 1:100 dilution in blocking buffer, Abgent, San Diego, CA) and Rabbit anti-COX-2 anti- body (CRM 306 A; 1:100 dilution in blocking buffer, Bio- care Medical, Concord, CA), ...
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Sphingosine kinase 1 is a potential therapeutic target for nasopharyngeal carcinoma
... CNE- 1 cells (Figure 2A), and a 5 μM dose achieved IC50 at approximately 30 hours after ...Moreover, Sphingosine Kinase Activity Assay Kit results showed that FTY720 induced the rapid inhibition of ...
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An novel role of sphingosine kinase 1 (SPHK1) in the invasion and metastasis of esophageal carcinoma
... for 1 h in citrate ...as 1; with moderate staining or strong staining in 25-50% of cells as 2; and with strong staining in > 50% of cells as ...
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Effect of the sphingosine kinase 1 selective inhibitor, PF-543 on arterial and cardiac remodeling in a hypoxic model of pulmonary arterial hypertension
... remodelling and pro-in fl ammatory changes. Current treatments for PAH are targeted at improving pulmonary haemodynamics, exercise tolerance and quality of life. Currently licenced drugs for treating PAH are all ...
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Sphingosine kinase 2 prevents the nuclear translocation of sphingosine 1-phosphate receptor-2 and tyrosine 416 phosphorylated c-Src and increases estrogen receptor negative MDA-MB-231 breast cancer cell growth : the role of sphingosine 1-phosphate receptor-4
... tyrosine kinase 1; Brms1, breast carcinoma metastasis suppressor 1; cyto, cytoplasmic preparation; ER, estrogen receptor; ERM, Ezrin, Radixin, Moesin protein; ERK, extracellular signal regulated ...
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Sphingosine 1-phosphate receptors and sphingosine kinase 1 : novel biomarkers for clinical prognosis in breast, prostate, and hematological cancers
... Clearly, the objective of S1P therapeutics is to eliminate the nega- tive prognostic effect of S1P receptors and SK1 on disease-specific survival and recurrence in cancer patients. In addition to the expression level of ...
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Proteasomal degradation of sphingosine kinase 1 and inhibition of dihydroceramide desaturase by the sphingosine kinase inhibitors, SKi or ABC294640, induces growth arrest in androgen-independent LNCaP-AI prostate cancer cells
... (3-(4-chlorophenyl)-adamantane- 1-carboxylic acid (pyridin-4-ylmethyl)amide), which is reported to be a selective inhibitor of SK2 activity, is in phase 2 clinical trials for diffuse B-cell ...(with ...
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Proteasomal degradation of sphingosine kinase 1 and inhibition of dihydroceramide desaturase by the sphingosine kinase inhibitors, SKi or ABC294640, induces growth arrest in androgen-independent LNCaP-AI prostate cancer cells
... (3-(4-chlorophenyl)-adamantane- 1-carboxylic acid (pyridin-4-ylmethyl)amide), which is reported to be a selective inhibitor of SK2 activity, is in phase 2 clinical trials for diffuse B-cell ...(with ...
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Sphingosine kinase 1 as an anticancer therapeutic target
... synthetic sphingosine analog FTY720 (2-amino-2- [2-(4-octylphenyl)]-1,3-propanediolhydrochloride), aka ...competitive sphingosine inhibitor of SK1, and its analog (S)-FTY720 ...
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Sphingosine kinase 2 in autoimmune/inflammatory disease and development of sphingosine kinase 2 inhibitors
... targeting sphingosine kinase 2 (SK2) in autoimmune/inflammatory ...isoform, sphingosine kinase 1 (SK1), which functions as a pro-inflammatory ...
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Expressions of Sphingosine 1 phosphate (S1P) Receptors, Sphingosine Kinases in Malignant Bone and Soft Tissue Tumors, and The role of Sphingosine Kinase 1 in Growth of MFH Cell Lines
... Reactions were carried out in 2 × PCR Master Mix (Ap- plied Biosystems Inc., Foster City, CA, USA) in a total volume of 50 μl containing 5 μl of cDNA, 0.4 μM of each PCR primer, 0.2 μM of TaqMan probe with passive ...
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Neuronal sphingosine kinase 2 subcellular localization is altered in Alzheimer’s disease brain
... Rabbit IgG (Immunologic) during 45 min at RT and treated with Liquid Fast Red (Abcam) for 30 min. Sections were counterstained with hematoxylin then mounted in Faramount Aqueous Mounting Medium (Dako). Once mounted, ...
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