Sphingosine Kinase 2
Antitumor effect of the novel sphingosine kinase 2 inhibitor ABC294640 is enhanced by inhibition of autophagy and by sorafenib in human cholangiocarcinoma cells
... Sphingosine kinase 2 (Sphk2) has an oncogenic role in cancer. A recently developed first-in-class Sphk2 specific inhibitor ABC294640 displays antitumor activity in many cancer models. However, the ...
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Sphingosine kinase 2 in autoimmune/inflammatory disease and development of sphingosine kinase 2 inhibitors
... targeting sphingosine kinase 2 (SK2) in autoimmune/inflammatory ...isoform, sphingosine kinase 1 (SK1), which functions as a pro-inflammatory ...
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Sphingosine kinase 2 prevents the nuclear translocation of sphingosine 1-phosphate receptor-2 and tyrosine 416 phosphorylated c-Src and increases estrogen receptor negative MDA-MB-231 breast cancer cell growth : the role of sphingosine 1-phosphate receptor-4
... tyrosine kinase 1; Brms1, breast carcinoma metastasis suppressor 1; cyto, cytoplasmic preparation; ER, estrogen receptor; ERM, Ezrin, Radixin, Moesin protein; ERK, extracellular signal regulated kinase; ...
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Targeting sphingosine kinase 2 (SphK2) by ABC294640 inhibits colorectal cancer cell growth in vitro and in vivo
... Surgery-isolated colon cancer tissues were thoroughly washed, non-cancerous surrounding tissues, if any, were separated carefully under microscopy, and were dis- carded. Clinical pathology reports confirmed those tissues ...
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Neuronal sphingosine kinase 2 subcellular localization is altered in Alzheimer’s disease brain
... presenilin 2 are regulated by retinoids [29, ...of sphingosine which is phosphorylated by nuclear SphK2, induced an improvement of memory process [19, 25] and a better survival of neuronal progeni- tors in ...
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Sphingosine kinase 2 supports the development of BCR/ABL-independent acute lymphoblastic leukemia in mice
... for 2 h at 56 °C and proteinase K was inactivated for 30 min at 85 °C prior to ...a 2% agarose (Sigma-Aldrich) gel stained with Midori Green Nucleic Acid solution (Bulldog Bio ...
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<p>Sphingosine kinase 2 inhibitor ABC294640 displays anti-epithelial ovarian cancer activities in vitro and in vivo</p>
... The effect of ABC294640 was assessed using mouse xeno- graft models to further explore whether it could inhibit the growth of EOC in vivo. SKOV3 cells were injected intraper- itoneally in the nude mice. After 7 days of ...
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An oncogenic role for sphingosine kinase 2
... human sphingosine kinases (SK1 and SK2) catalyze the generation of the pleiotropic signaling lipid sphingosine 1-phosphate, these enzymes appear to be functionally ...
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Photoacoustic microscopy reveals the hemodynamic basis of sphingosine 1-phosphate-induced neuroprotection against ischemic stroke
... that sphingosine 1-phosphate (S1P), a bioactive metabolite of sphingolipids, may play an important role in the pathophysiological processes of cerebral hypoxia and ...of sphingosine kinase 2 ...
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Crystal structure of sphingosine kinase 1 with PF-543
... of sphingosine to sphingosine-1-phosphate (S1P), the fi nal step of S1P ...two sphingosine kinases: Sphingo- sine Kinase 1 (SPHK1) is mostly localized to the cytosol while Sphingosine ...
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Sphingosine 1-phosphate receptors and sphingosine kinase 1 : novel biomarkers for clinical prognosis in breast, prostate, and hematological cancers
... lipid sphingosine 1- phosphate (S1P), the enzyme sphingosine kinase 1 (that catalyses S1P formation) and S1P-specific G protein-coupled ...that sphingosine kinase 1 and S1P receptors are ...
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Etoposide upregulates survival favoring sphingosine-1-phosphate in etoposide-resistant retinoblastoma cells
... for sphingosine-1-P and SPHK1 and 2 in other diverse ...of sphingosine-1-P-levels and a reduced proliferation in prostatic tumor cells, pancreatic tumor cells and leukemia cells (25-28, ...
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Sphingosine Kinase Regulates the Sensitivity of Dictyostelium discoideum Cells to the Anticancer Drug Cisplatin
... repeating pipettor that automatically mixes dilutions (Matrix Technologies Corp., Hudson, N.H.). A total of 15 l of each diluted sample was added to 20 l of K. aerogenes food source in 24-well plates containing ...
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Sphingosine Kinase Regulates Microtubule Dynamics and Organelle Positioning Necessary for Proper G1/S Cell Cycle Transition in Trypanosoma brucei
... The results of cytotoxicity assays with SPHK inhibitors (Fig. 7) suggest that TbSPHK may be a novel drug target. Both DMS and safingol have a 10-fold therapeutic index versus human cells. DMS is useful for the treatment ...
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Effect of sphingosine kinase modulators on interleukin-1β release, sphingosine 1-phosphate receptor 1 expression and experimental autoimmune encephalomyelitis : FTY720 analogues and inflammation
... Figure 4. The effect of the Caspase-1 inhibitor Ac-YVAD-CHO or the PP2A inhibitor, okadaic acid on the enhancement of IL-1β release by compound 5 in the presence of LPS. Differentiated U937 cells were treated with ...
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Effect of the sphingosine kinase 1 selective inhibitor, PF-543 on arterial and cardiac remodeling in a hypoxic model of pulmonary arterial hypertension
... EDTA, 2% w/v SDS) (pH 6.7) containing 12.5% v/v glycerol, 0.25% w/v bromophenol blue, and 50 mM dithiothreitol] was used for SDS-PAGE and Western blotting. Proteins were separated on a 10% (v/v) acrylam- ...
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Leptin induces upregulation of sphingosine kinase 1 in oestrogen receptor negative breast cancer via Src family kinase mediated, janus kinase 2 independent pathway
... file 2). Pharmacological inhibition of ERK1/2 and SFKs was veri- fied using specific siRNA pools against ERK1/2, Src and Fyn (Figure S13-S15 in Additional file ...
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Emerging role of sphingosine-1-phosphate signaling in head and neck squamous cell carcinoma
... targets. Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid involved in inflamma- tion, tumor progression, and ...two sphingosine kinases ...OSCC, sphingosine-1-phosphate, S1P, ...
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A reflection of the lasting contributions from Dr. Robert Bittman to sterol trafficking, sphingolipid and phospholipid research.
... intracellular calcium concentrations [86]. In the early 1990’s LPA was identified as a mitogen that acted through a pertussis sensitive G-protein coupled receptor(s) and p21Ras in fibroblasts [87-89]. Tigyi et al. [90] ...
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Sphingosine kinase 1 as an anticancer therapeutic target
... An accumulation of evidence indicates that the SK/S1P/S1PR signaling pathway plays a crucial role in various cancers and in sphingolipid-mediated drug resistance. Elevated expres- sion of SK1 leads to an oncogenic ...
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