sputum specimens
Rapid Detection of Mycobacterium tuberculosis and Pyrazinamide Susceptibility Related to pncA Mutations in Sputum Specimens through an Integrated Gene to Protein Function Approach
... Because of the many problems associated with the phenotypic methods for testing PZA susceptibility of M. tuberculosis, such as their lengthiness, high cost and low accuracy, DNA sequencing of the pncA gene is being used ...
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Alkaline decontamination of sputum specimens adversely affects stability of mycobacterial mRNA
... study sputum specimens collected serially from patients throughout their course of treatment for pulmonary ...in sputum, even after the specimens were culture nega- ...
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Evaluation of bleach sedimentation for sterilising and concentrating Mycobacterium tuberculosis in sputum specimens
... the sputum without shaking [14], shaking at reg- ular intervals for 15 minutes [16] or continuous shaking for 10 minutes [15] before dilution with water followed by ...
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Evaluation of Amplicor PCR for direct detection of Mycobacterium tuberculosis from sputum specimens
... in sputum. A total of 532 specimens from 270 patients were decontaminated and stored at 4 or 2 75 & C until assayed by ...445 specimens from which ...Amplicor-positive specimens, 15 were ...
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ISOLATION AND ANTIBIOTIC SENSITIVITY OF KLEBSIELLA PNEUMONIAE FROM SPUTUM SPECIMENS OF PATIENTS OF FEDERAL MEDICAL CENTRE (F M C), UMUAHIA, ABIA STATE, NIGERIA
... Klebsiella pneumoniae was isolated from 8 sputum specimens produced by males and 4 produced by females (Table 1). This indicates that the infection was found more in males than female patients sampled. This ...
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Comparison of amplified Q beta replicase and PCR assays for detection of Mycobacterium tuberculosis
... culture-positive specimens or have been hindered by the pres- ence of inhibitors in patient sputum specimens, leading to a significant number of false-negative reactions (3, 7, 23, 26, ...
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FREQUENCY OF MORAXELLA CATARRHALIS FROM PATIENTS WITH LOWER RESPIRATORY TRACT INFECTION IN KHARTOUM STATE, SUDAN
... in sputum specimens among patients with lower respiratory tract ...200 sputum specimens collected from Khartoum state in Alshaab hospital during January to June ...of sputum ...
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A Pyrosequencing Based Approach to High Throughput Identification of Influenza A(H3N2) Virus Clades Harboring Antigenic Drift Variants
... respiratory specimens (throat swabs, nasal swabs and washes, nasopharyngeal swabs, and sputum specimens) (n ⫽ 27) that were collected between 1 October 2013 and 1 July 2015 in the United States and ...
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Novel methodology to assess sputum smear microscopy quality in private laboratories
... for sputum smear mi- croscopy tests in the same way as all other laboratory customers and submitted the spiked sputum specimens over a three day period, such that each lab under evaluation received 7 ...
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Diagnostic Accuracy and Utility of FluoroType MTBDR, a New Molecular Assay for Multidrug Resistant Tuberculosis
... smear-negative specimens. Within the group of 322 sputum specimens that tested positive for the ...the sputum specimen, only 4 results discrepant by FluoroType were proven to be truly ...from ...
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Molecular detection of multidrug-resistant tuberculosis among smear-positive pulmonary tuberculosis patients in Jigjiga town, Ethiopia
... In this study, institution-based cross-sectional study design was employed. This study was conducted in a health insti- tution in which data for associated risk factors for drug resistance were collected using a ...
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Loop Mediated Isothermal Amplification for Direct Detection of Mycobacterium tuberculosis Complex, M avium, and M intracellulare in Sputum Samples
... We used a novel nucleic acid amplification method, LAMP, for detection of M. tuberculosis complex, M. avium, and M. intracellulare either as culture isolates or in sputum specimens. The mycobacterial ...
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Sputum Mycobacterium tuberculosis mRNA as a Marker of Bacteriologic Clearance in Response to Antituberculosis Therapy
... in sputum, we developed as- says for additional targets in anticipation of identifying genes expressed at higher levels than fbpB and hspX mRNA, so that a higher number of mRNA molecules per organism would be ...
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Comparison of Two Culture Methods for Detection of Tobramycin Resistant Gram Negative Organisms in the Sputum of Patients with Cystic Fibrosis
... Typical sputum culture methods quantitate the relative amounts of each distinct morphotype, followed by antibiotic susceptibility testing of a single colony of each ...morphotype. Sputum specimens ...
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Q beta replicase amplified assay for detection of Mycobacterium tuberculosis directly from clinical specimens
... processing. Sputum specimens were processed as outlined in Fig. 1. Specimens were first processed by the laboratories according to their standard digestion and decontamination ...processed ...
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Monitoring Treatment of Patients with Pulmonary Tuberculosis: Can PCR Be Applied?
... For all 22 patients, the lines of regression showed a decline in MTB/MCC OD ratios during treatment. Most patients suf- fering from moderate disease reached baseline levels at the end of a standard 6 to 8 months of ...
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A Review of Microbiologic Isolates of Adults with Lower Respiratory Tract Infection
... of sputum isolation rate ...processing sputum specimen. 73.7% of the total sputum specimens collected for analysis was ...of sputum isolates and antibiogram ...
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A Study on Accuracy of Respiratory Cytology for Cancer Diagnosis.
... include sputum, bronchial brushing, bronchial washing, bronchioalveolar lavage, transbronchial needle aspiration, transthoracic fine needle aspiration and endoscopic ultrasonography guided fine needle ...
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Simplified sample processing combined with a sensitive one tube nested PCR assay for detection of Pneumocystis carinii in respiratory specimens
... clinical specimens (312 bronchoalveolar lavage, 403 sputum or induced sputum, and 34 other specimens) from 507 patients (295 human immunodeficiency virus [HIV]-infected and 164 ...
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Evaluation of Xpert MTB/RIF for Detection of Tuberculosis from Blood Samples of HIV-Infected Adults Confirms Mycobacterium tuberculosis Bacteremia as an Indicator of Poor Prognosis
... concentrated sputum samples were examined by iLED fluorescence microscopy (auramine O), with any pos- itive results confirmed by Ziehl-Neelsen (ZN) ...staining. Sputum samples used for mycobacterial culture ...
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