stem-cell-derived neurons
Significant transcriptional changes in 15q duplication but not Angelman syndrome deletion stem cell-derived neurons
... duplication neurons and a recent study looking at gene expression changes in post- mortem brain samples from both Dup15q syndrome and idiopathic ASD ...DPSC-derived neurons to postmortem cor- tical ...
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Manifestation of Huntington’s disease pathology in human induced pluripotent stem cell-derived neurons
... human neurons, the complexity of the molecular mecha- nisms underlying HD pathology, and the lack of ad- equate animal ...somatic cell reprogramming technology, as well as the development of differentiation ...
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CGG-repeat dynamics and FMR1 gene silencing in fragile X syndrome stem cells and stem cell-derived neurons
... post-mitotic neurons using the previously described dual SMAD in- hibition method [38] with some ...using Stem- Pro®Accutase® and re-plated into ultra low-attachment dishes (Corning, Corning, NY) in ...
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Human Embryonic Stem Cell-Derived Neurons Are Highly Permissive for Varicella-Zoster Virus Lytic Infection
... shingles. Cell-free VZV has been difficult to obtain, both for in vitro studies and for vaccine ...other cell types in vitro and that the number of total virus genomes rel- ative to the number of viral ...
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Cellular and molecular characterization of multiplex autism in human induced pluripotent stem cell-derived neurons
... pluripotent stem cell lines (iPSCs) from affected individuals or by using CRISPR/Cas9-based gene editing to engineer ASD- associated mutations into wild-type PSCs ...ASD-derived neurons, ...
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Human induced pluripotent stem cell derived neurons as a model for Williams-Beuren syndrome
... The derivation and pluripotency characterization of WBS iPS cell lines has been reported previously [11]. Neuronal differentiations were carried out as described previously [13] but with minor modifications, which ...
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Varicella-Zoster Virus Infects Human Embryonic Stem Cell-Derived Neurons and Neurospheres but Not Pluripotent Embryonic Stem Cells or Early Progenitors
... FIG 1 Naïve hESC are not infected by VZV. (A) Schematic of the infection of hESC and their neurally differentiated derivatives. VZV-infected MeWo or ARPE cells (green) were treated with mitomycin C to prevent ...
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Role of the JNK Pathway in Varicella-Zoster Virus Lytic Infection and Reactivation
... onic stem cell-derived neurons and that this activation of JNK is essential for effi- cient viral protein expression and ...sensory neurons from neu- ral crest cells and established a ...
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In vitro myelin formation using embryonic stem cells
... of neurons. Myelination enables neurons to transmit information more quickly and more efficiently and allows for more complex brain functions; yet, remarkably, the underlying mechanism by which myelination ...
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In Vitro Differentiation of Adipose Derived Stem Cells Into Functional Dopaminergic Neurons
... the cell body alongside a single long axon-like processes in some differentiated ...spheres derived from ADSCs resembled to neurospheres, we evaluated the NSCs-specific antigen nestin in ADSCs colonies ...
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Long term culture of pluripotent stem cell derived human neurons on diamonds" A substrate for neurodegeneration research and therapy
... Brain Computer Interfaces (BCI) currently represent a field of intense research aimed both at under- standing neural circuit physiology and at providing functional therapy for traumatic or degenerative neurological ...
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Varicella-Zoster Virus Expresses Multiple Small Noncoding RNAs
... and neurons generated from the hESC line H9 were prepared and grown as detailed in reference ...for stem-loop RT-PCR were performed using a recombinant VZV expressing green fluorescent protein (GFP) as an ...
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Comparative genomic analysis of embryonic, lineage converted and stem cell derived motor neurons
... induced neurons, are expressed by many types of neurons, not just the cortical neurons used as the reference cell ...reference cell transcriptome is highly heterogeneous (Marro et ...
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Aberrant Virion Assembly and Limited Glycoprotein C Production in Varicella-Zoster Virus-Infected Neurons
... differentiated neurons derived from pluripotent stem cells appear healthy at 2 weeks after infec- tion with varicella-zoster virus (VZV), and the cell culture medium contains no infectious ...
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Automated microfluidic cell culture of stem cell derived dopaminergic neurons
... Differentiated neurons projecting their processes can be observed in the culture lane and in the area occupied by the meniscus, located in part of the medium ...positive neurons (red) were characterised ...
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Down-regulation of ghrelin receptors on dopaminergic neurons in the substantia nigra contributes to Parkinson’s disease-like motor dysfunction
... pluripotent stem cell (iPSC)-derived dopaminergic (DAnergic) neurons generated from patients carrying parkin gene (PARK2) mutations compared to those from healthy ...DAnergic neurons of ...
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Materials for stem cell factories of the future
... Xeno-free defined conditions for culture of human embryonic stem cells, neural stem cells and dopaminergic neurons derived from them.. Derivation of human embryonic stem cells in defined[r] ...
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Stem cell derived basal forebrain cholinergic neurons from Alzheimer’s disease patients are more susceptible to cell death
... astrocytes. Neurons also express ApoE [45,46], and our data suggest that neuronal ApoE4 increases se- cretion of Aβ42, the more fibrillar form that is more likely to aggregate and act as the core for amyloid pla- ...
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Wnt5a treated midbrain neural stem cells improve dopamine cell replacement therapy in parkinsonian mice
... DA neurons than did conventional FGF2-treated ...of stem cell–derived DA neurons in vivo and define Wnt5a-treated neural stem cells as an efficient and safe source of DA ...
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Characterisation of neurons derived from a cortical human neural stem cell line CTX0E16
... CTX0E16 neurons exhibit typical pyram- idal neuronal ...glutamatergic neurons is their ability to generate unipolar pyramidal neuronal morphology: interneurons display multipolar morphologies [17, 33, ...
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