Stem Cells and Cell Signalling
Activin/Nodal signalling in stem cells
... inhibits cell growth and proliferation) (Massagué, 2004): it regulates cell cycle progression in many cell types and acts as a central pathway for mediating cytostatic ...These cell cycle ...
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Jak/Stat signalling in niche support cells regulates dpp transcription to control germline stem cell maintenance in the Drosophila ovary
... follicle stem cells, such as fs(1)Yb, piwi, wingless and hedgehog, are known to be expressed in CpCs (Cox et ...of signalling pathways that co-exist in the ...support cells in direct contact ...
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ATP-induced Ca2+-signalling mechanisms in the regulation of mesenchymal stem cell migration
... mature cell types, but the underlying mechanisms still remain not fully ...microglial cells [94] occurs in the central and peripheral nervous ...same cells are equipped with multiple ATP release ...
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Predictions for optimal mitigation of paracrine inhibitory signalling in haemopoietic stem cell cultures
... of cell-to-surface-area ratio, rather than cell seeding con- ...example, cells in a culture seeded at 10 5 cells/ml are packed much closer together in a standard tissue culture 24-well-plate ...
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Hh signalling is essential for somatic stem cell maintenance in the Drosophila testis niche
... Jak/Stat signalling is responsible for the maintenance and activity of cells that contribute to the GSC niche, and Hh signalling promotes the proliferation of stem cells that provide ...
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Neotendon formation induced by manipulation of the Smad8 signalling pathway in mesenchymal stem cells
... tenocyte-like cell formation (Figure ...C2C12 cells BMP4 induces ALP activity equally well in the presence of recombinantly expressed Smad8, Smad1, and ...C2C12 cells through the activation of Smad1 ...
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β1 integrins activate a MAPK signalling pathway in neural stem cells that contributes to their maintenance
... plated cells (3000 cells/well of a 24-well ...by cells not expressing β-galactosidase) were not included in the ...β1/wt cells were passaged at least ten times and then incubated overnight in ...
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LIF-dependent survival of embryonic stem cells is regulated by a novel palmitoylated Gab1 signalling protein
... reduced cell death and extended the viability of ESCs. When cells were treated with an inhibitor of the key nutrient sensor and anabolic regulatory enzyme mTOR, Gab1 β expression improved ESC survival, ...
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Live imaging of ERK signalling dynamics in differentiating mouse embryonic stem cells
... group cells based on their ERK activity profile during the 16 h ...ERK signalling responses, with transient or sustained ERK activity following the initial peak ...between signalling and rate of exit ...
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Dynamic Notch signalling regulates neural stem cell state progression in the Drosophila optic lobe
... Notch signalling pathway is a key regulator of cell-cell communication required for stem cell self-re- newal and differentiation ...neighbouring cell, the Notch intracellular ...
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IGF1R signalling in testicular germ cell tumour cells impacts on cell survival and acquired cisplatin resistance.
... precursor cells of TGCT [1,19,36]. IGF1R signalling is also required for the development of PGCs and ...IGF signalling is also required for maintaining the germ cell population in postnatal ...
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Retinoic acid orchestrates fibroblast growth factor signalling to drive embryonic stem cell differentiation
... Embryonic stem (ES) cells fluctuate between self-renewal and the threshold of ...differentiation. Signalling via the fibroblast growth factor (Fgf)/Erk pathway is required to progress from this ...
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Evidence for bystander signalling between human trophoblast cells and human embryonic stem cells
... immunofluorescence, cells were fixed with 4% paraformaldehyde (Sigma Aldrich) in PBS for 15 minutes at 4 ...°C. Cells were permeabilised by washing three times in PBST; PBS supplemented with ...1). ...
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Mitochondrial fission as a driver of stemness in tumor cells : mDIVI1 inhibits mitochondrial function, cell migration and cancer stem cell (CSC) signalling
... MCF7 cells also impedes their propagation under anchorage- independent ...of cells rather than be generated through clonal ...differentiated cells can also exhibit sphere-forming capability, and ...
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Escort cells generate a dynamic compartment for germline stem cell differentiation via combined Stat and Erk signalling
... Despite their clear phenotypic consequences, the mechanistic aspects of how the Stat and Egfr pathways control protrusions remain elusive. On the one hand, ECs must invade between tightly packed germ cells and ...
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JNK signalling is necessary for a Wnt and stem cell dependent regeneration programme
... JNK signalling in promoting posterior regeneration, without global effects on regeneration or effects on stem cell proliferation or stem cell ...of stem cells after ...
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Activation of Notch1 signalling promotes multi-lineage differentiation of c-KitPOS/NKX2.5POS bone marrow stem cells: implication in stem cell translational medicine
... endogenous stem cells within heart tissue, termed cardiac stem cells (CSCs), offers great potential for stem cell research ...desired cell numbers for in vivo ...
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Integrin Rac signalling for mammary epithelial stem cell self renewal
... °C. Cells were spun for 1 min at 300 rpm, and the pellet was re-digested with collagenase/trypsin mix for an additional 30 min while the supernatant was spun for 3 min at 800 ...completed, cells were spun ...
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Enrichment of therapeutic hematopoietic stem cell populations from embryonic stem cells
... electroporation. Cell culture, electroporation and selection of ESC were carried out using the method described by Bronson et ...a cell line derived from 129/Ola mice with an ~50kb deletion in its Hprt gene ...
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Mesenchymal stem cells as a reference cell for HLA-typing
... isolated cells: An image for the cultured isolated cells was shown in ...These cells were spindle- shape and ...isolated cells were related to MSCs. The isolated cells were positive for ...
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