Stimulation of monocyte-derived dendritic cells
Immature monocyte derived dendritic cells gene expression profile in response to Virus Like Particles stimulation
... The MDDCs differentiation process induced by HIV-VLPs, in fact, results in the reduced transcriptional levels of genes associated with phagocytosis and pathogen recogni- tion. In parallel, an activation of genes ...
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SAMHD1 Limits HIV-1 Antigen Presentation by Monocyte-Derived Dendritic Cells
... CTL stimulation can occur through exogenous presentation of antigens, this process is less efficient than endogenous presenta- tion (4, 6, ...T cells in the presence of CTL, whereas the transmission of ...
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Generation of Mature Monocyte-Derived Dendritic Cells in the Presence of Heparin and Monocyte Conditioned Medium: Phenotypic and Functional Comparison
... Mixed lymphocyte reaction. To address whether heparin or MCM-stimulated DC would be able to induce a proliferative response in allogeneic MLR, T cells were stimulated with heparin or MCM or both treated DC at a ...
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Varying Effects of Different β-Glucans on the Maturation of Porcine Monocyte-Derived Dendritic Cells
... 0.02]). Stimulation of MoDCs with 5 g of curdlan/ml also significantly reduced their phagocytic ability (P ⫽ ...after stimulation of MoDCs with 10 g of LPS/ml, no effect was seen at the lowest ...
6
The tumor-associated antigen RHAMM (HMMR/CD168) is expressed by monocyte-derived dendritic cells and presented to T cells
... these cells was further confirmed by ...malignant cells but is also found at varying levels in different normal cells and tissues, including testis and spermatocytes, placenta, thymus, the eye, parts ...
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Quantitative proteomic changes in LPS activated monocyte derived dendritic cells : a SWATH MS study
... Changes in cytokines and cytokine-induced proteins in LPS-stimulated moDCs. It was apparent from the pathway analyses that a number of moDC proteins associated with cytokine signalling and in particular, interferon and ...
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Effects of Filovirus Interferon Antagonists on Responses of Human Monocyte-Derived Dendritic Cells to RNA Virus Infection
... human monocyte-derived DC (MDDC) responses upon stimulation induced through the RIG-I-like receptor ...after stimulation of MDDCs with RIG-I ...
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Vpx-independent lentiviral transduction and shRNA-mediated protein knock-down in monocyte-derived dendritic cells
... of cells should express the transgene to avoid the need for further selection steps that may require cell sorters or time consuming drug-based selection, which by itself can affect function and phenotype of the ...
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Differentiation of Monocyte Derived Dendritic Cells in End Stage Renal Disease is Skewed Towards Accelerated Maturation
... This hypothesis was recently supported in pa- tients undergoing renal transplantation, where the afore existing deficiencies in incidence and func- tion of precursor DC were reversed [27]. More- over, serum from uremic ...
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The Effects of Bovine Viral Diarrhea Virus and Bovine Herpesvirus Type 1 on Monocyte-Derived Dendritic Cells
... immediate-early (IE), early (E), and late (L). In IE gene expression, stimulation occurs by virion component bTIF. There are two transcription units in IE transcription. IE transcription unit 1 encodes function ...
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Hypoxia potentiates monocyte-derived dendritic cells for release of tumor necrosis factor alpha via MAP3K8
... TLR stimulation in turn also affects HIF signaling ...TLR stimulation can pro- mote the stabilization of HIF as well as up-regulate its expression ...TLR stimulation or hypoxia [22] and this ...
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Transient receptor potential vanilloid-2 mediates the effects of transient heat shock on endocytosis of human monocyte-derived dendritic cells
... Collectively, these findings strongly implicate that the cellular effects of heat shock on human DCs are mediated by TRPV2 ion channels. Intriguingly, on human mast cells, similar findings were recently published by ...
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Differential Activation of Human Monocyte-Derived and Plasmacytoid Dendritic Cells by West Nile Virus Generated in Different Host Cells
... upon stimulation with both viruses, whereas pDCs are unable to synthesize IFN- ␣ upon stimulation with the mos- quito cell-derived ...immune cells, which drive the initial innate response to ...
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Subviral Dense Bodies of Human Cytomegalovirus Stimulate Maturation and Activation of Monocyte-Derived Immature Dendritic Cells
... of stimulation of the T cells from seropositive do- nors by DB-treated DC were comparable to those seen with DC that had been incubated with virions of both RV-HB5 and RV- ...(5–9). Stimulation by ...
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Histamine regulates multiple functional activities of murine and monocyte-derived human dendritic cells via different receptors
... The hallmark of activated DCs is the production of cytokines and chemokines, which may act in an autocrine or paracrine manner. Previous results demonstrated that histamine induced altered cytokine production in DCs, ...
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In vitro suppression of immune responses using monocyte derived tolerogenic dendritic cells from patients with primary Sjögren's syndrome
... suppress stimulation of naïve T cells in the presence of fully matured DC loaded with pSS-related autoantigens Ro52, Ro60 and ...regulatory cells (Tr1) and B regulatory cells (Breg) ...Breg ...
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DIFFERENTIATION OF MONOCYTE DERIVED DENDRITIC CELLS IN SERUM FREE CONDITIONS
... dendritiform cells of an increased accessory potency showing reduced Mel ...into monocyte derived dendritic cells ...the cells will acquire most of DC criteria; functionally, ...
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HIV 2 infects resting CD4+ T cells but not monocyte derived dendritic cells
... T cells. Unstimulated CD4+ T cells were not productively infected by HIV-2, but viral replication was triggered upon lymphocyte activation in a Vpx-dependent ...
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Antitumor Efficacy of Human Monocyte-Derived Dendritic Cells: Comparing Effects of two Monocyte Isolation Methods
... 0.01% of all blood cells). To overcome this problem, high amounts of human DCs are generated in vitro from pre- cursors, CD14 pos (CD14 + ) monocytes or CD34 + cells, isolated from peripheral blood [7]. ...
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Monocyte Differentiation To Dendritic Cells Protocol
... tumor cells are an antigen source with direct clinical relevance to cancer ...of monocyte-derived Dendritic Cells moDC ...Human Monocyte-derived Dendritic ...