The strategy for oligonucleotide-mediated mutagenesis
Targeted mutagenesis of simian virus 40 DNA mediated by a triple helix-forming oligonucleotide.
... Although the SV40 plasmid vector is smaller than the lambda vector and so is a less complex target, the difference in the frequency of targeted mutations cannot be explained by a differe[r] ...
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Mutagenesis as a Genetic Research Strategy
... chemical mutagenesis, the rationale of experiments with chemicals was: ‘‘If, as we assume, a mutation is a chemical process, the knowledge of the reagents capa- ble of initiating this process should throw light ...
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Characterization of Mos1-Mediated Mutagenesis in Caenorhabditis elegans
... Insertional mutagenesis with a heterologous transposon provides a method to rapidly determine the molecular identity of mutated ...Mos1-mediated mutagenesis we examined the conditions influencing ...
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Antisense oligonucleotide mediated therapy of spinal muscular atrophy
... mismatch base pairing and have off-target effects. Furthermore, transcribed RNAs form secondary structures within microseconds of emerging from polymerase II (23-25). Thus, low annealing efficiency of ASOs with targets ...
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Lethal Mutagenesis of Poliovirus Mediated by a Mutagenic Pyrimidine Analogue
... and mutagenesis, presumably a reflection of rP nucleotide formation by a nucleotide salvage ...lethal mutagenesis as an antiviral strategy, suggest that rPMP prodrugs may be highly efficacious ...
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Germline mutagenesis mediated by Sleeping Beautytransposon system in mice
... Since the development of the SB transposon system, several other transposon systems have been reported to be active in mammalian cells, including Minos [23] (isolated from Drosophila hydei), piggyBac [24] (from the moth ...
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Optimising antisense oligonucleotide-mediated exon skipping for Duchenne muscular dystrophy
... 54 C HAPTER 2 2.1 Utrophin upregulation Instead of trying to bring back dystrophin into muscle, an alternative strategy is to increase the expression of the dystrophin homologue utrophin. In the mdx mouse model it ...
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Agrobacterium tumefaciens-mediated transformation of Aspergillus aculeatus for insertional mutagenesis
... aculeatus cells when using conidia, protoplasts, or germlings. On average, 30 transformants per 10 4 conidia or 217 transformants per 10 7 conidia were obtained under the optimized conditions when A. tumefaciens ...
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Antisense oligonucleotide–mediated MDM4 exon 6 skipping impairs tumor growth
... that MDM4 protein, but not mRNA, levels are elevated in 65% of human melanoma samples has raised the possibility that total mRNA measurements have dramatically underestimated the frequency of MDM4-expressing cancers. Our ...
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CRISPR/Cas9-mediated mutagenesis of phytoene desaturase in diploid and octoploid strawberry
... Conclusion The primary aim of this work was to generate a set of detailed data using a visible marker that will be of rele- vance for gene editing in Fragaria of single- and multi- copy genes for which there is no ...
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Ethyl Methanesulfonate (EMS) Mediated Mutagenesis of Cucumber (Cucumis sativus L )
... LD% = (1 − Germination rates of treated seeds/Germination rates of Control) × 100 2.3. EMS-Mediated Mutagenesis Mutant populations were constructed based on the work flow in Figure 1. In brief, wild type ...
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CRISPR/Cas9-mediated mutagenesis of phytoene desaturase in diploid and octoploid strawberry
... The primary aim of this work was to generate a set of detailed data using a visible marker that will be of rele- vance for gene editing in Fragaria of single- and multi- copy genes for which there is no visible ...
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The Mechanism of Nucleotide Excision Repair-Mediated UV-Induced Mutagenesis in Nonproliferating Cells
... NER-dependent mutagenesis may be more ...of mutagenesis occurs in the absence of cell division, it potentially provides a way for cells to escape either adverse growth conditions or normal growth ...
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Bidirectional-Genetics Platform, a Dual-Purpose Mutagenesis Strategy for Filamentous Fungi
... Currently, experimental approaches for investigating fungal gene function via genome alteration are divided into two. Tar- geted gene replacement (TGR) via homologous recombination (HR) is a straightforward reverse ...
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Inefficient cationic lipid-mediated siRNA and antisense oligonucleotide transfer to airway epithelial cells in vivo
... Thus, although the stability of the protein may play a role, we suggest that poor transfection is more likely the reason for the lack of effect. The efficiency of siRNA and antisense inhibition is sequence dependent and ...
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Targeted Mutagenesis of Guinea Pig Cytomegalovirus Using CRISPR/Cas9-Mediated Gene Editing
... CRISPR/Cas9 mutagenesis is a technically simple alternative to BAC recombineering that eliminates the need for a BAC interme- ...CRISPR/Cas9 mutagenesis in as few as three pas- ...
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Random Transposon-Mediated Mutagenesis of the Essential Large Tegument Protein pUL36 of Pseudorabies Virus
... In summary, with this random mutagenesis approach we further identified and delineated functional domains in pUL36 of PrV. The region located in the central part of the protein comprising amino acids 1540 to 1987 ...
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Genetic Analysis of Bacillus subtilis spo Mutations Generated by Tn917-Mediated Insertional Mutagenesis
... Although the collection of spo mutations described here adds significantly to the total of known loci, and thus helps to define the set of genes whose products are required for spo[r] ...
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A novel retroviral mutagenesis screen identifies prognostic genes in RUNX1 mediated myeloid leukemogenesis
... Furthermore, the NBEAL2 gene which is located near CCDC12 is already recognized in familial platelet disorders with a predisposition to MDS/AML [20, 21]. In summary, our D171N retroviral shuttle vector mutagenesis ...
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Evaluation and rational design of guide RNAs for efficient CRISPR/Cas9-mediated mutagenesis in Ciona
... sgRNA mutagenesis activity and used this information for the rational design of all possible sgRNAs targeting the Ciona ...CRISPR/Cas9-mediated mutagenesis in somatic cells of electroporated ...the ...
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