Streptomyces lividans
Four thiol-oxidoreductases involved in the formation of disulphide bonds in the Streptomyces lividans TK21 secretory proteins
... Streptomyces lividans is a well-known gram-positive bacterium, and an efficient secretor of proteins with industrial application ...in Streptomyces lividans TK21 secretome contained two or ...
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Two heterologously expressed Planobispora rosea proteins cooperatively induce Streptomyces lividans thiostrepton uptake and storage from the extracellular medium
... of Streptomyces lividans, SL-40, -48, -85, - 120, isolated from a ...one Streptomyces livi- dans subclone, named ...of Streptomyces nogalater involved in nogalamycin resistance ...
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Modification of genetic regulation of a heterologous chitosanase gene in Streptomyces lividans TK24 leads to chitosanase production in the absence of chitosan
... hosts: Streptomyces lividans TK24 (the host used so far in most works involving actinobacterial chitosanase stu- dies) and a mutant harbouring an in-frame deletion in csnR gene ( ΔcsnR, formerly described ...
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Secretory production of tetrameric native full-length streptavidin with thermostability using Streptomyces lividans as a host
... [13-15]. Streptomyces lividans is the most versatile host among this genus for the production of useful ...S. lividans as the ...S. lividans has great ability to express heterologous proteins, ...
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New approaches to achieve high level enzyme production in Streptomyces lividans
... Streptomyces lividans 1326 and its derivative strains, GSAL1 [16], ΔxlnR, and ΔbxlR were used as ...S. lividans 1326 has been published [39] and is available at StrepDB ...S. lividans ...
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Novel insights regarding the sigmoidal pattern of resistance to neomycin conferred by the aphII gene, in Streptomyces lividans
... Streptomyces lividans TK24 strains transformed with 38 pIJ487-derived plasmids, each carrying a 300 bp DNA fragment with promoter activity of various strength were used in this study (Ward et ...as ...
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The metabolic switch can be activated in a recombinant strain of Streptomyces lividans by a low oxygen transfer rate in shake flasks
... Streptomyces lividans is the bacterial system of choice within the genus Streptomyces for heterologous protein and glycoprotein production, even though the most stud- ied species of the genus is ...
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p-Hydroxycinnamic acid production directly from cellulose using endoglucanase- and tyrosine ammonia lyase-expressing Streptomyces lividans
... Background: p-Hydroxycinnamic acid (pHCA) is an aromatic compound that serves as a starting material for the production of many commercially valuable chemicals, such as fragrances and pharmaceuticals, and is also used in ...
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Large-scale production of a thermostable Rhodothermus marinus cellulase by heterologous secretion from Streptomyces lividans
... Background: The gene encoding a thermostable cellulase of family 12 was previously isolated from a Rhodothermus marinus through functional screening. CelA is a protein of 260 aminoacyl residues with a 28-residue ...
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Bioconversion of agricultural lignocellulosic residues into branched-chain fatty acids using Streptomyces lividans
... of Streptomyces lividans TK 24 to grow on agricultural lignocellulosic residues from Brassica napus and Helianthus annuus, some of the main oleaginous crops in Europe, was ...
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Enhanced production of a recombinant, thermostable [alpha]-amylase in streptomyces lividans: Effects of plasmid construction and culture condition
... Another possible reason for the differences seen would be loss of plasmid from the cells or reduced plasmid copy number. The spore stocks used to inoculate these flasks were produced at the beginning of the project, ...
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The O-mannosylation and production of recombinant APA (45/47 KDa) protein from Mycobacterium tuberculosis in Streptomyces lividans is affected by culture conditions in shake flasks
... Mycobacterium tuberculosis is the main bacterial pathogen causing latent infection in more than a third of the world population, and is also developing resistance to virtually every new drug used to treat tuberculosis, ...
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Lipid metabolism in Streptomyces lividans TK24
... that Streptomyces glaucescens tetracenomycin C production and fatty acid synthesis could share the malonyl-CoA:ACP synthase I (from the PKS pathway) in vitro, indicating that the pathways are closely structurally ...
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Hyper secretion of Thermobifida fusca β-glucosidase via a Tat-dependent signal peptide using Streptomyces lividans
... S. lividans. After BGL-secreting S. lividans was constructed using pTYM18 involving XCs for BGL secretion, we introduced pUC702 carrying XCs or Plds for BGL secretion into the constructed strain, and ...
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Recombinant production of Streptococcus equisimilis streptokinase by Streptomyces lividans
... In order to establish the expression and secretion of SK from Streptococcus equisimilis ATCC9542 in S. lividans, the skc-2 gene was amplified by PCR using chromosomal DNA as template and finally cloned in ...
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Transcriptomic and fluxomic changes in Streptomyces lividans producing heterologous protein
... S. lividans TK24 lacks the cytoplasmic transhydrogenase UdhA (gene not pre- sent, [20]) which typically oxidizes NADPH into NADP + , an alternate route must be ...S. lividans TK24, but re-oxidation of NADPH ...
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Metabolomics investigation of recombinant mTNFα production in Streptomyces lividans
... in Streptomyces is produced from glucose 6-phosphate via the activity of myo-inositol- 1-phosphate synthase (EC ...in Streptomyces during cellular differentiation and ...different Streptomyces ...
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The examination of organic acid production during the growth of 'streptomyces lividans' TK24
... levels of up to 2.8g-L‘^ DCW at 96h (Figure 3.50), which was greater than in glucose- nitrate medium (2.1g-L'^). DNA analysis indicated that replicatory growth would have continued beyond 120h and the end of biomass ...
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Comprehensive subcellular topologies of polypeptides in Streptomyces
... Streptomyces lividans has several advantages that moti- vate its use as an expression ...S. lividans TK24 is attractive as a host for genetic manipulation, because it is insensitive to methylated ...
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CHARACTERIZATION OF VIRULENT BACTERIOPHAGES FOR STREPTOMYCES GRISEOFLAVUS ISOLATED FROM SOI
... of Streptomyces were the source of useful and consequently profitable antibiotics and antiviral directed unprecedented attention to this genus (Mansour et ...1988). Streptomyces are also known as producers ...
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