Studying reporter gene expression using stable transfectants
Development of Stable Rotavirus Reporter Expression Systems
... prototype reporter RV-NLuc strain revealed that the NLuc transgene was lost after long-term passage in cell ...NSP1 gene from NSP1-NLuc chimeric viruses increases the stability of reporter genes by ...
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Exploring the eukaryotic gene expression machinery using a single-cell yeast gene expression reporter
... identify candidate drugs with high-throughput screening. Although this can drastically complicate the screening process in terms of cell growth, contamination, etc., it is most often considered to be advantageous because ...
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Plasmid vector based generation of transgenic mesenchymal stem cells with stable expression of reporter gene in caprine
... studies using vectors based on oncogenic retroviruses have been attempted to transduce MSCs, but there have been problems due to a number of ...transgene expression [48,49], possibly due to the inactivation ...
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Indexing TNF-αgene expression using a gene-targeted reporter cell line
... Cell-based gene reporter assay systems were developed as an alternative system amenable to HTS over 10 years ago, and have been widely used to study transcription and gene ...detectable ...
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Modulation of Reporter EGFP Gene Expression by a Disease-Associated Human Intra-Intronic Minisatellite Upon Transient and Stable Transfection
... the reporter gene EGFP under euka ryotic pro moter ROSA 26, and different a lle lles of hu man minisatellite UPS29 for both transient and stable transfection of F9 ce lls induced to differentiate, ...
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Utilization of the herpes simplex virus type 1 latency-associated regulatory region to drive stable reporter gene expression in the nervous system.
... of using the endogenous LAT promoter, with all associ- ated upstream and downstream regulatory elements, to drive reporter gene expression during HSV ...nonuniform expression of LATs ...
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Establishment of Stable Reporter Expression for In Vivo Imaging of Nuclear Factor-κB Activation in Mouse Liver
... erase expression in extra-hepatic ...NF-κB reporter to the hepatic tissues [15]. However, transient NF- κ B reporter expression precludes further application for monitoring NF- κ B activation ...
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Towards the Development of a MagA Reporter Gene Expression Construct for Magnetic Resonance Imaging
... luciferase reporter gene expression construct using the hNISmin and investigate its induction by ...MagA gene construct using HA- and myc- epitopes cloned onto the 5ʹ′ and 3ʹ′ ...
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Stable, High-Level Expression of Reporter Proteins from Improved Alphavirus Expression Vectors To Track Replication and Dissemination during Encephalitic and Arthritogenic Disease
... by using the highly efficient Thosea asigna virus (TaV) 2A-like protease ...Transgene expression, stability, and virulence of the vectors in animals were then ...compared. Using nLuc and fLuc as ...
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Creation of stable Pseudomonas aeruginosa promoter–reporter fusion mutants using linear plasmid DNA transformation
... infections. Reporter-gene systems and construction of mutants defective in specific functions are useful tools for studying the cellular physiology and virulence of this ...approach using ...
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Heat Induced Reporter and Therapeutic Gene Expression in Cancer Gene Therapy
... buffer using a Mini-Beadbeater, and murine IL-12 concentrations in tumor extracts were determined using a mIL-12 ELISA ...the reporter gene and mIL-12 production for the therapeutic ...
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Using a Single Fluorescent Reporter Gene to Infer Half-Life of Extrinsic Noise and Other Parameters of Gene Expression
... of gene expression and obtain information about sources of nongenetic ...of gene expression, as well as the strength and half-life of extrinsic noise from single ...
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Studying genetic determinants of natural variation in human gene expression using Bayesian ANOVA
... Determining gene expression profiles For our purposes, a gene expression profile is a multi-gene pattern of expression values by which a given individual may be assigned ...
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Evaluation of GFP reporter utility for analysis of transcriptional slippage during gene expression
... Epigenetic effects of ribosomal and transcriptional frameshifting play important role in regulating gene expression processes as it has clearly emerged from the accumulated and still growing knowledge in ...
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In Vivo magnetic resonance imaging of xenografted tumors using FTH1 reporter gene expression controlled by a tet-on switch
... the reporter gene FTH1 located downstream of Tet-On was expressed, and iron accumulated inside the cells, generating MRI ...the reporter FTH1 gene. By using the Tet-On switch, ...
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The Human Sodium Iodide Symporter as a Reporter Gene for Studying Middle East Respiratory Syndrome Coronavirus Pathogenesis
... to studying infectious diseases may provide insight into ...SPECT-compatible reporter gene into a virus may provide insight into mechanisms of pathogenesis and viral ...tomography reporter ...
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In vivo Monitoring of microRNA Biogenesis Using Reporter Gene Imaging
... optical reporter gene system for finding microRNA targets can identify the target mRNAs and elucidate the functional relationship between the microRNAs and their target ...miRNA. Reporter gene ...
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Simultaneous tracking of movement and gene expression in multiple Drosophila melanogaster flies using GFP and DsRED fluorescent reporter transgenes
... flies using both visible and fluorescent ...3xP3-GFP reporter was assayed. The 3xP3-GFP reporter contains a promoter with multiple binding sites for the Eyeless (Pax6 homolog) transcription factor, ...
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Regulation of Closterovirus Gene Expression Examined by Insertion of a Self-Processing Reporter and by Northern Hybridization
... Kinetics of gene expression driven by the tagged promoters. The high sensitivity and specificity of the GUS assays per- mitted a comparative study of the kinetics of reporter ex- pression in tagged ...
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Evidence for expression of promoterless GFP cassette: Is GFP an ideal reporter gene in biotechnology science?
... To explain the result, we hypothesized either of several reasons could be the case: a) Auto transduction of the cells with the viruses produced during the process of packaging. In this view, the viral RNA could be ...