Survivin promoter
Delivery of a survivin promoter-driven antisense survivin-expressing plasmid DNA as a cancer therapeutic: a proof-of-concept study
... Abstract: Survivin is a member of the inhibitor-of-apoptosis proteins ...of survivin promotes cancer cell survival and induces chemotherapeutic drug resistance, making it an attractive target for new ...
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Original Article Targeting of pertechnetate imaging of HepG2 hepatocellular carcinoma through the transduction of the survivin promoter controls the sodium iodide symporter
... the survivin promoter, which would be more suitable for disseminated tumors and distant metastatic ...CMV promoter showed no tumor specificity, and the normal liver tissues exhibited signifi- cant ...
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Original Article Improved radioiodine-131 imaging of prostatic carcinoma using the sodium iodide symporter gene under control of the survivin promoter
... of survivin promoter into prostate cancer PC-3 cells, and investigated the in vitro radioio- dine uptake, and evaluated the in vivo optimal time of radioiodine ...
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Survivin promoter-regulated oncolytic adenovirus with Hsp70 gene exerts effective antitumor efficacy in gastric cancer immunotherapy
... The adenoviral vector used herein was created using the previously generated Survivin promoter- regulated oncolytic adenoviruses AdSurp-EGFP [10] and AdCMV-Hsp70 [7]. Full-length Hsp70 cDNA, which contains ...
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Survivin promoter rs9904341 polymorphism is associated with tumor stage and grade in patients with bladder cancer
... the survivin promoter –31 G/C (rs9904341) polymorphism was not only associated with the risk of bladder cancer but also related with invasive and high-grade ...
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Original Article Radionuclide imaging and therapy in malignant melanoma after survivin promoter-directed sodium iodide symporter gene transfer in vitro and in vivo
... Abstract: This study aimed to develop a gene expression targeting method specific for the imaging and therapy of malignant melanoma A375 cells using the sodium iodide symporter gene under control of the survivin ...
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Correlation between the levels of survivin and survivin promoter-driven gene expression in cancer and non-cancer cells
... tumor-specific promoter (TSP) exhibits selective high activity in tumor cells (a “tumor on” ...(AFP) promoter for hepatocarcinoma [55]. Recently, a novel TSP, the survivin promoter, that ...
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Anti-tumor activity of selective inhibitors of XPO1/CRM1- mediated nuclear export in diffuse malignant peritoneal mesothelioma: the role of survivin
... that survivin is an essential component in DMPM cell response to SINE-mediated XPO1/CRM1 ...cytoplasmic survivin levels and, after an initial survivin nuclear accumulation, also to a progressive ...
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Targeted Hsp70 expression combined with CIK-activated immune reconstruction synergistically exerts antitumor efficacy in patient-derived hepatocellular carcinoma xenograft mouse models
... Because Survivin is barely expressed in normal tissues but is highly expressed in most malignant tumors, its expression is highly selective, making it a broad spectrum molecular target for the diagnosis and ...
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Survivin a radiogenetic promoter for glioblastoma viral gene therapy independently from CArG motifs
... of survivin RNA transcripts in cells [57, 58, ...Since survivin pro- moter has no TATA box, these sequences are involved in gene expression and cell cycle ...where survivin promoter, with the ...
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SIRT1 expression is associated with poor prognosis of lung adenocarcinoma
... UK), Survivin (rab- bit monoclonal, ab134170; Abcam plc), and VEGF (mouse monoclonal, ab1316; Abcam plc) as primary antibodies for 60-minute staining, and it continued with the MaxVision™ HRP-Polymer anti-Rabbit ...
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Survivin expression is associated with lens epithelial cell proliferation and fiber cell differentiation
... of Survivin protein to the nuclei of these cells, where it associated with marginalized chromatin indicative of the early stages of ...that Survivin may play an important role in vertebrate lens ...of ...
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Identification of a Movement Protein of the Tenuivirus Rice Stripe Virus
... Constructs and procedures for complementation experiments. The move- ment-defective PVX tagged with the -glucuronidase (GUS) gene (pPVX.GUS- Bsp) (27) was used for complementation experiments. Construct pBI-P25, ex- ...
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Stage-dependent prognostic impact of molecular signatures in clear cell renal cell carcinoma
... were used. After incubation with ABC-reagent (Vectastain Elite ABC Kit anti-mouse; Vector Laboratories, Inc.), DAB (3,3’-Diaminobenzidin) was used for visualization and Meyer’s Hemalaun for counterstaining. For every ...
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The oncofetal gene survivin is re expressed in osteoarthritis and is required for chondrocyte proliferation in vitro
... For survivin immunoblotting, proteins were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis using 10% gels and blotted onto Immobilon P Membranes (Millipore, Bedford, ...human survivin ...
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Survivin co-ordinates formation of follicular T-cells acting in synergy with Bcl-6
... Chromatin immunoprecipitation (ChIP) analysis Human PBMC were isolated by density gradient separation on Lymphoprep (Axis-Shield PoC As, Norway), and cultured in the presence of Concanavalin A (0.625 μg/ml, ...
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A study into the potential role of Survivin localization in resistance to drug-induced apoptosis
... with Survivin-GFP, no protec- tive effect to therapeutic agents was seen in samples trans- fected with Survivin-L98A-GFP, where the over-expressed protein is restricted to the ...the Survivin-NES ...
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Original Article Targeting survivin suppresses proliferation and invasion of retinoblastoma cells in vitro and in vivo
... with survivin siRNA was associated with increased cell death, we treated Y79 cell lines with survivin siRNA complexed to Lipofectamine 2000, and measured apoptosis by annexin V staining and flow ...
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Original Article Coexpression of survivin and livin predicts lymph node metastasis and poor prognosis in cervical cancer
... high survivin/livin coexpression had shorter overall survival and disease-free sur- vival time than those with low or no survivin/ livin ...Besides, survivin/livin coex- pression was correlated with ...
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Original Article Ultrasound-targeted microbubble destruction promotes lipofectamine-mediated shRNA-survivin transfection efficiency thereby inhibiting survivin expression and inducing apoptosis of HepG2 cells
... To determine the effect of combining UTMD with Lipo transfection on transfection efficiency in HepG2, the cells were divided into five groups: untreated, shRNA-survivin, shRNA-sur- vivin with Lipo, ...
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