SV40 large T antigen
Study of SV40 large T antigen nucleotide specificity for DNA unwinding
... SV40 LT is a replicative helicase that recognizes origin DNA and assembles into a hexameric/double hexameric helicase to initiate DNA replication of the viral genome. LT is unique in that LT alone can open up the ...
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Quantitation of CD8+ T-Lymphocyte Responses to Multiple Epitopes from Simian Virus 40 (SV40) Large T Antigen in C57BL/6 Mice Immunized with SV40, SV40 T-Antigen-Transformed Cells, or Vaccinia Virus Recombinants Expressing Full-Length T Antigen or Epitope Minigenes
... epitope-specific T-lymphocyte ...by T lympho- cytes responding to immunodominant epitopes have shown that both limited (2, 17, 19, 21, 22) and highly variable (20, 30, 57) usage can be detected, depending ...
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An N-terminal deletion mutant of simian virus 40 (SV40) large T antigen oligomerizes incorrectly on SV40 DNA but retains the ability to bind to DNA polymerase alpha and replicate SV40 DNA in vitro.
... Recombinant-baculovirus-expressed T antigen (37) has proved highly active in cell-free SV40 DNA replication assays (39, 49), as a result of the replication-active under-phosphory- lated form of the ...
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Mutation of the cyclin-dependent kinase phosphorylation site in simian virus 40 (SV40) large T antigen specifically blocks SV40 origin DNA unwinding.
... A mutant T antigen carrying alanine in place of Thr-124 T124A also failed to replicate SV40 DNA in vitro and was defective in its ability to initiate replication in vitro Knowledge of eu[r] ...
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SV40 Large T Antigen Disrupts Embryogenesis of Canine and Porcine Somatic Cell Nuclear Transfer Embryo
... Simian virus 40 large T (SV40LT) is a viral protein from simian virus 40, a member of Polyomaviridae. SV40LT is known to interact with various host proteins, including p53 and retinoblastoma, which inhibit ...
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Identification of novel protein interactors of the SV40 large T antigen using the yeast two-hybrid system
... utant T-Ag (Jat and Sharp, ...tsA58 T-Ag is rapidly inactivated (Gonos et al., 1996). T- Ag and B ubl co-immunoprecipitated only at the permissive temperature in tsa cells (Figure ...of T-Ag ...
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Interaction of the Transcription Factor TFIID with Simian Virus 40 (SV40) Large T Antigen Interferes with Replication of SV40 DNA In Vitro
... for T antigen: impli- cations for viral ...to T antigen antagonizes viral DNA replica- tion in vitro ...between T-antigen hexamers in unwinding viral DNA ...on T ...
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Mechanisms of interference with simian virus 40 (SV40) DNA replication by trans-dominant mutants of SV40 large T antigen.
... By cotransfecting CV-1 cells with an origin-defective plasmid encoding wild-type T antigen and a replicationcompetent plasmid encoding mutant T antigen, we saw that the level of interfer[r] ...
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Only a minor fraction of plasma membrane-associated large T antigen in simian virus 40-transformed mouse tumor cells (mKSA) is exposed on the cell surface.
... 3926 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The bulk of simian virus 40 SV40 large T antigen in SV40-infected and -transformed cells localizes within the cell [r] ...
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The amino-terminal functions of the simian virus 40 large T antigen are required to overcome wild-type p53-mediated growth arrest of cells.
... At 32°C, these cells arrest in the G phase of the cell cycle, and the ability of the SV40 large T antigen to overcome this temperature-dependent block in cell division was examined.. The[r] ...
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Requirements for species-specific papovavirus DNA replication.
... DISCUSSION We have contructed polyomavirus-SV40 hybrid replication oris and isolated mouse cell lines and monkey cell lines that express either polyomavirus or SV40 large T antigen to te[r] ...
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Binding of p53 and p105-RB is not sufficient for oncogenic transformation by a hybrid polyomavirus-simian virus 40 large T antigen.
... The amino acid sequence of SV40 large T antigen SV T from residues 337 to 517 is shown aligned to the corresponding region of two other p53-binding papovavirus large T antigens, those of[r] ...
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Free and viral chromosome-bound simian virus 40 T antigen: changes in reactivity of specific antigenic determinants during lytic infection.
... 635 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Simian virus 40 SV40 large T antigen TAg, both free and bound to mature 70S and replicating 90S SV40 chromosomes, wa[r] ...
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A novel translational regulation function for the simian virus 40 large-T antigen gene.
... the SV40 large-T antigen might be me- diated by the mRNA that encodes the large-T antigen, rather than by the ...mutant large-T plasmid (pR137) was ...
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Replication of simian virus 40 origin-containing DNA during infection with a recombinant Autographa californica multiple nuclear polyhedrosis virus expressing large T antigen.
... (SV40) large T antigen was surprisingly found to induce the efficient replication of a transfected plasmid containing an SV40 ...(i) T antigen, since replication of ...
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Association of simian virus 40 T antigen with replicating nucleoprotein complexes of simian virus 40.
... Recently, Mann and Hunter 30 detected SV40 large T antigen in the fraction of a sucrose gradient containing mature 70S NPCs by immunoprecipitation of T antigen labeled with [3S]methionin[r] ...
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Three domains in the simian virus 40 core origin orchestrate the binding, melting, and DNA helicase activities of T antigen.
... Simian virus 40 large tumor antigen requires three core replication origin domains for DNA unwinding and replication in vitro.. Simian virus 40 SV40 DNA replication: SV40 large T antigen[r] ...
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High-Throughput Cell-Based Screen for Chemicals That Inhibit Infection by Simian Virus 40 and Human Polyomaviruses
... for SV40 infection. We developed a sensitive cell-based assay for SV40 infection of a human cancer cell line and used it to screen a chemical library for small molecules that inhibit infection ...an ...
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Mutual functional antagonism of the simian virus 40 T antigen and the hepatitis B virus trans activator.
... Positive and negative regulation of transcription in vitro: enhancer binding protein AP-2 is inhibited by SV40 T antigen.. Production of simian virus 40 large tumor antigen in bacteria: [r] ...
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Effects of in vitro dephosphorylation on DNA-binding and DNA helicase activities of simian virus 40 large tumor antigen.
... 22 showed that dephosphorylation by alkaline phosphatase increases the affinity of T antigen to the SV40 binding site II and, furthermore, that the activity of dephosphorylated T antigen[r] ...
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