Synechococcus elongatus
Modulation of the balance of fatty acid production and secretion is crucial for enhancement of growth and productivity of the engineered mutant of the cyanobacterium Synechococcus elongatus
... Results: By modulating the expression level of the acyl–acyl carrier protein thioesterase and raising the light inten‑ sity during cultivation, FFA secretion rates comparable to those obtained with the other ...
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Removal of the product from the culture medium strongly enhances free fatty acid production by genetically engineered Synechococcus elongatus
... Unlike TAG production using green algae, produc- tion of free fatty acids (FFA) using genetically engineered cyanobacteria results in secretion of the product into the medium [8–10]. If the rate of FFA secretion is ...
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CRISPR/Cas9 mediated targeted mutagenesis of the fast growing cyanobacterium Synechococcus elongatus UTEX 2973
... complementary target sequence, where Cas9 creates a double stranded break [18]. The CRISPR system can be engineered for genome editing by reprogramming the spacer sequences to be complementary to the genetic target [21]. ...
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Photoautotrophic production of polyhydroxyalkanoates in a synthetic mixed culture of Synechococcus elongatus cscB and Pseudomonas putida cscAB
... strain Synechococcus elongatus cscB has recently been shown to export sucrose on a level comparable to sugar cane ...S. elongatus cscB accumulates remarkable amounts of sucrose as a compatible ...
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Iterative cycle of widely targeted metabolic profiling for the improvement of 1-butanol titer and productivity in Synechococcus elongatus
... Results: In the present study, a new strain (DC7) with an improved activity of PduP enzyme, was constructed using BUOHSE as the background strain. DC7 showed a 33% increase in 1-butanol production compared to BUOHSE. For ...
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Adjustments to Photosystem Stoichiometry and Electron Transfer Proteins Are Key to the Remarkably Fast Growth of the Cyanobacterium Synechococcus elongatus UTEX 2973
... level, Synechococcus elongatus UTEX 2973 (Synechococ- cus 2973) is nearly identical to the model cyanobacterium Synechococcus elongatus PCC 7942 (Synechococcus 7942) with only 55 single ...
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Synechococcus elongatus PCC7942 secretes extracellular vesicles to accelerate cutaneous wound healing by promoting angiogenesis
... cyanobacterium Synechococcus elongatus (S. elongatus) PCC 7942 is a unicellular, obligate photoautotroph that represents a preeminent model for studying photosynthesis and circadian rhythms [5, ...S. ...
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Synechococcus elongatus as a model of photosynthetic bioreactor for expression of recombinant β-glucosidases
... of Synechococcus elongatus capable of expressing high levels of a heterologous β-glucosidase from a microorganism from the Amazonian ...S. elongatus, once nickel induced T7 RNA polymerase expression ...
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Effect of salinity on some physiological and biochemical responses in the cyanobacterium Synechococcus elongatus
... of Synechococcus elongatus to salt stress were ...S. elongatus was significantly inhibited under salt stress on days 5, 9 and 12 after ...S. elongatus on day 12 in presence of ...S. ...
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Metabolic engineering of Synechococcus elongatus PCC 7942 for improvement of 1,3-propanediol and glycerol production based on in silico simulation of metabolic flux distribution
... isoprene [16, 17], 1,2-propanediol (1,2-PDO) [18], and glycerol [19, 20]. In addition, we successfully developed Synechococcus elongatus PCC 7942 to produce 1,3-pro- panediol (1,3-PDO) by introduction of a ...
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Isolation, Purification and Characterization of Nucleoids from Synechococcus elongatus PCC 7942
... the Synechococcus elongatus (se) PCC 7942 strain were isolated and purified in presence of spermidine at low salt concentrations by sucrose density gradient ...
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Engineering the fatty acid synthesis pathway in Synechococcus elongatus PCC 7942 improves omega-3 fatty acid production
... Results: To characterize the function of some enzymes involved in the saturated fatty acid synthesis in cyanobac‑ teria, we genetically engineered Synechococcus elongatus PCC 7942 by[r] ...
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Alkane biosynthesis by Aspergillus carbonarius ITEM 5010 through heterologous expression of Synechococcus elongatus acyl-ACP/CoA reductase and aldehyde deformylating oxygenase genes
... In this study we describe the heterologous expression of the recently identified cyanobacterial pathway for long chain alkane biosynthesis, involving the reduction of fatty acyl-ACP to fatty aldehyde and the subsequent ...
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The primary transcriptome of the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973
... S. elongatus UTEX ...S. elongatus PCC 7942 [27], but it was greatly up-regulated in S. elongatus UTEX 2973 (Table ...S. elongatus UTEX ...S. elongatus UTEX 2973 would be degraded and ...
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Perspectives in the Coordinate Regulation of Cell Cycle Events in Synechococcus
... of Synechococcus were based on fundamentally explicit experimental data and model ...in Synechococcus, the characterization of the cell cycle events and the isolation of a light responsive initiation factor ...
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Engineering a cyanobacterium as the catalyst for the photosynthetic conversion of CO2 to 1,2-propanediol
... Figure 3 Optimization of the 1,2-Propanediol production by exploiting different acetol-reducing enzymes. LH22 and LH23 were constructed which harbored C. beijerinckii and T. brockii. sADH. A) methylglyoxal synthase ...
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Thermoacclimation and genome adaptation of the membrane lipidome in marine Synechococcus
... Pittera, Justine, Jouhet, Juliette, Breton, Solène, Garczarek, Laurence, Partensky, Frédéric, Maréchal, Éric, Nguyen, Ngoc A., Doré, Hugo, Ratin, Morgane, Pitt, Frances Diana, Scanlan, David J. and Six, Christophe. ...
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The transcriptomes of Xiphinema index and Longidorus elongatus suggest independent acquisition of some plant parasitism genes by horizontal gene transfer in early branching nematodes
... L. elongatus), Clade 10 (Parasitaphelenchidae—Bursaphelenchus ...L. elongatus contain GH12 cellulases that are likely to have been acquired from ...L. elongatus GH12 sequence was only identified in a ...
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Structural and functional consequences of removing the N-terminal domain from the magnesium chelatase ChlH subunit of Thermosynechococcus elongatus
... T. elongatus to find out whether this head domain is indeed at the N-terminus, and to determine whether it represents an autonomous region of ChlH, in the sense that the rest of the ChlH folds correctly in its ...
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An invasive crustacean in Tasmania, Australia : a study on the introduced porcelain crab Petrolisthes elongatus
... Petrolisthes elongatus) (Infraorder Anomura) are an aspect dominant species, achieving high population densities in benthic habitats with limited apparent impacts, and have been introduced to North America, South ...
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