Synthetic lethal screen using PARG inhibitors
PARG deficiency is neither synthetic lethal with BRCA1 nor PTEN deficiency
... Discussion Using cancer cell lines genetically deficient in BRCA1 and/or PTEN and siRNA-mediated depletions, our study shows that PARG deficiency is neither synthetic lethal with BRCA1 nor ...
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ATR inhibitors as a synthetic lethal therapy for tumours deficient in ARID1A.
... inconsistent with one another, it is possible that the TOP2A defect in ARID1A mutant tumours has relatively moderate effects (compared to a profound homologous recombination defect for example) on the structure of the ...
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Synthetic lethal targeting of PTEN mutant cells with PARP inhibitors
... HR using a reporter assay. This comprised a previously validated synthetic DNA substrate that bears an inducible double strand DNA break (DSB; Saeki et al, ...
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Synthetic lethal targeting of PTEN mutant cells with PARP inhibitors
... HR using a reporter assay. This comprised a previously validated synthetic DNA substrate that bears an inducible double strand DNA break (DSB; Saeki et al, ...
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Synthetic Lethal Interactions With Oncogenic KRAS
... shRNA screen was performed across 216 human cancer cell lines, and identified 59 candidate genes that may be selectively essential in KRAS-mutant cancer ...(2) using C911 seed-control shRNAs to evaluate ...
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Using bacterial inclusion bodies to screen for amyloid aggregation inhibitors
... to screen for such ...of inhibitors of copper and zinc promoted ag- ...bind synthetic amyloid Aβ peptides or to modulate their aggregation and/or toxicity [33–37] but have never been assayed before ...
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A Synthetic Lethal Screen Identifies a Role for the Cortical Actin Patch/Endocytosis Complex in the Response to Nutrient Deprivation in Saccharomyces cerevisiae
... (B) Prk1⌬ cells transformed with either multi- copy SIW14 under the control of the GAL1 promoter in pYES2 or the pYES2 vector alone. Transformed cells were grown to mid- exponential phase on YEP-raffinose to which 1% ...
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Predicting synthetic lethal interactions using conserved patterns in protein interaction networks.
... Of the drugs tested, three showed differential effects on the BAF180-deficient cells when compared to the control cells. PBRM1 mutant cells were more sensitive to both the PARP inhibitor and, to a lesser extent, ABL1 ...
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Identification of potential synthetic lethal genes to p53 using a computational biology approach
... thetic lethal interaction with p53. Although the genome- wide synthetic lethal RNAi screening strategy has been demonstrated to be effective in identifying potential targets for cancer therapeutic ...
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High-throughput simultaneous screen and counterscreen identifies homoharringtonine as synthetic lethal with von Hippel-Lindau loss in renal cell carcinoma
... synthetic lethal target, VHL, is inactivated in over 80% of ccRCC, and therefore any compounds identified in our screen have the potential to be broadly active against ...the screen and ...
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Predicting synthetic lethal genetic interactions in Saccharomyces cerevisiae using short polypeptide clusters
... pair using ClustalW [24]. Then, we built a hidden Markov model (HMM) using the output of the multiple sequence alignment as the ...sequences using HMM model against all 357,256 short polypeptide ...
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Exploiting synthetic lethal interactions for targeted cancer therapy
... display synthetic lethality with p53 in the context of DNA-damaging chemotherapy, might suggest that synthetic lethality with p53 is a generalizable feature of all cell cycle checkpoint signaling pathways, ...
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CDK1 Is a Synthetic Lethal Target for KRAS Mutant Tumours.
... candidate synthetic lethal ...later using Cell Titre Glo ...each screen by the calculation of Z ’ values and Spearman rank correlation between replicates, we estimated the effect on cell ...
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Synthetic lethal interactions of EPHB6 in breast cancer cells
... experiments using both shRNAs and the CRISPR/Cas9 system to target SRC in EPHB6-defiecient cells, thereby indicating that chemical inhibitors should be the next ...
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Development of WWP2 ubiquitin ligase inhibitors using biophysical, structural and synthetic approaches.
... stating there is an allosteric binding site between the N and C lobe which can be targeted for inhibition by small molecules. 6.5 Future work This thesis provides a framework for various avenues that could be pursued in ...
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Synthetic Lethal Screen Demonstrates That a JAK2 Inhibitor Suppresses a BCL6-dependent IL10RA/JAK2/STAT3 Pathway in High Grade B-cell Lymphoma.
... PCR using MyFi polymerase (Bioline, London, UK) and the primers 5 ⬘ -taaggtac- cccttgtccaccttg-3 ⬘ (forward, with a KpnI site) and 5 ⬘ -cct- gctagcttcgaactcagc-3 ⬘ (reverse, with a NheI ...orientation using ...
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Model-driven discovery of synergistic inhibitors against E. coli and S. enterica serovar Typhimurium targeting a novel synthetic lethal pair, aldA and prpC.
... apparent synthetic lethality between aldA and prpC explains why aldA is not singly essential, which is the computed phenotype using the most current version of the ...
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Hocke, Sandra (2016): A synthetic lethal screen identifies ATR-inhibition as a novel therapeutic approach for POLD1-deficient cancers. Dissertation, LMU München: Medizinische Fakultät
... the synthetic lethal interactions between ATR and the DNA-repair genes identified in this ...by using chemical ATR-inhibitors on POLD1-depleted DLD1 parental ...
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Synthetic Lethal Targeting of ARID1A-Mutant Ovarian Clear Cell Tumors with Dasatinib.
... example, using additional OCCC cell lines (both ARID1A wild type and mutant) as established orthotopic tumours (as opposed to non- established tumours shown in Figure 6) would extend the observations made ...
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Synthetic lethal therapies for cancer: what's next after PARP inhibitors?
... ATR inhibitors. Defining the different modes by which synthetic lethal effects operate could, in principle, allow novel synthetic lethal interactions to be predicted, rather than ...
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