T-ANTIGEN
Tiny T antigen: an autonomous polyomavirus T antigen amino-terminal domain.
... Electrophoretic analysis of tiny T antigen. T antigens were analyzed by the discontinuous gel procedure (59) as described in “Sigma Technical Bulletin no. MWM-100.” After electrophoresis (usually 3.5 ...
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Adding an Rb-binding site to an N-terminally truncated simian virus 40 T antigen restores growth to high cell density, and the T common region in trans provides anchorage-independent growth and rapid growth in low serum concentrations.
... more T-antigen activities in addition to Rb binding and the transforming function(s) in the T com- mon region are needed for anchorage-independent growth and efficient growth in low-serum ...a ...
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Simian virus 40 large T antigen contains two independent activities that cooperate with a ras oncogene to transform rat embryo fibroblasts.
... of T-antigen fusion proteins to cooperate with ras to transform ...of T antigen that must be at- tained to initiate ras cooperation is not ...wild-type T antigen and T176-708 ...
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Polyomavirus middle T-antigen NPTY mutants.
... 5770 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest A polyomavirus middle T-antigen MTAg mutant containing a substitution of Leu for Pro at amino acid 248 has previousl[r] ...
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Localization of the phosphorylations of polyomavirus large T antigen.
... Hydroxylamine cleavage of large T antigen labeled with [35S]methionine yielded peptides that matched electrophoretically the two phosphorylated fragments.. A third fragment 60K fragment [r] ...
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Asp-286----Asn-286 in polyomavirus large T antigen relaxes the specificity of binding to the polyomavirus origin.
... To determine whether binding of large T antigen was responsible for the defective replication of these DNAs and, more generally, to show that binding of large T antigen to the origin was[r] ...
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Characterization of the DNA-Binding Properties of the Origin-Binding Domain of Simian Virus 40 Large T Antigen by Fluorescence Anisotropy
... the T-antigen OBD would in- crease substantially its affinity for DNA, as we have observed for the related DBD of the papillomavirus E1 helicase ...the T- antigen OBD would indeed increase its ...
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Dissection of H-2Db-restricted cytotoxic T-lymphocyte epitopes on simian virus 40 T antigen by the use of synthetic peptides and H-2Dbm mutants.
... The inability of mutant class I antigens of bml3SV and bml4SV cells to present an endogenously processed T antigen to CTL clone Y-1 and that of bml4SV cells to present the T antigen to t[r] ...
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Simian Virus 40 Large T Antigen and Two Independent T-Antigen Segments Sensitize Cells to Apoptosis following Genotoxic Damage
... these T antigens were gener- ated and examined for evidence of apoptosis following 5-FU ...expressing T antigen containing the amino acid substitution D44N (REF/T1–708D44N⫹Ras) or H42Q ...
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Simian virus 40 small t antigen activates the carboxyl-terminal transforming p53-binding domain of large T antigen.
... large T and p53 for SV40-mediated cellular transformation is firmly estab- lished (16, 23, 52, 56, 59, 73, 74), although the functional consequences of this interaction are not fully understood and are still a ...
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T-antigen kinase inhibits simian virus 40 DNA replication by phosphorylation of intact T antigen on serines 120 and 123.
... Traugh, and Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest DISCUSSION This study demonstrates that the ability of T-antigen kinase, an isoform of casein kinase I, to in[r] ...
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Analysis of Simian Virus 40 Chromosome—T-Antigen Complexes: T-Antigen Is Preferentially Associated with Early Replicating DNA Intermediates
... 281-295 0022-538X/83/100281-15S02.00/0 Copyright © 1983, American Society for Microbiology Analysis of Simian Virus 40 Chromosome-T-Antigen Complexes: T-Antigen Is Preferentially Associa[r] ...
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Polyoma viral middle T-antigen is required for transformation.
... The smallest continuous tract of polyoma Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest To determine whether small or middle T-antigen or both of polyoma virus is requ[r] ...
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Regions and Activities of Simian Virus 40 T Antigen That Cooperate with an Activated ras Oncogene in Transforming Primary Rat Embryo Fibroblasts
... The C-terminal Ras cooperation segment T251–708 binds p53 (6) and prevents p53 from activating a p53-responsive promoter (Fig. 6). However, binding and inactivation of p53 as a transcriptional transactivator were not ...
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Dimerization of simian virus 40 T-antigen hexamers activates T-antigen DNA helicase activity.
... tumor antigen (T anti- gen) (11), with all other replication factors provided by the host ...that T antigen first binds the viral origin of replication (ori) in an ATP-dependent reaction (4, ...
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Multimerization of polyomavirus middle-T antigen.
... middle-T antigen were transiently or stably expressed in NIH 3T3 ...expressed T antigens. As shown in Fig. 2A, full-length middle-T antigen was specif- ically recognized by all the ...
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Immunological Cross-Reactivity Between Simian Virus 40 Large T Antigen and D2 Hybrid T Antigen
... Proteins were immunoprecipitated from these extracts by using the antiserum produced in rabbits against purified D2 hybrid T antigen rabbit anti-D2, and these proteins were compared with[r] ...
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Transformation of precrisis human cells by the simian virus 40 cytoplasmic-localization mutant pSVCT3 is accompanied by nuclear T antigen.
... DISCUSSION The T antigen of pSVCT3, a mutant of SV40 that is defective for the transport of T antigen to the nucleus of monkey cells, transforms precrisis human cells as efficiently as t[r] ...
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Model for T-Antigen-Dependent Melting of the Simian Virus 40 Core Origin Based on Studies of the Interaction of the Beta-Hairpin with DNA
... the T-ag helicase domain and ssDNA, it is likely that the T-ag-obds are positioned such that they are in the center of the complex, where they maintain certain interactions with ...the T-ag-obd to ...
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Sequence Requirements for the Assembly of Simian Virus 40 T Antigen and the T-Antigen Origin Binding Domain on the Viral Core Origin of Replication
... for T-ag binding in the presence of AMP-PNP, the 48-bp site II ⫹ EP oligonucleotide, is at the top of the table while the weakest substrate, the 47-bp control oligonucleotide, is at the ...of T-ag assembled ...
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