T cell factor-1 (TCF-1)
Nuclear Wiskott–Aldrich syndrome protein co-regulates T cell factor 1-mediated transcription in T cells
... and cell suspension prepared with the use of 100-μm strainers ...For cell frac- tionation, the Nuclear/Cytosol Fractionation Kit #K266-25 (BioVision) was ...Total cell lysates were prepared by ...
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Comorbidity as an independent prognostic factor in elderly patients with peripheral T-cell lymphoma
... A total of 34 males (60.7%) and 22 females (39.3%) with a median age of 67 years (range: 60–80 years) were included in this study. With regard to histological subtypes, 41 (73.2%) patients had PTCL-NOS, nine (16.1%) had ...
5
Nuclear factor of activated T-cell isoform expression and regulation in human myometrium
... Whole cell lysate proteins were separated using 10 % Tris-Glycine precast gels (Invitrogen) using the XCell SureLock ™ Mini-Cell system ...diluted 1:1000 or β-actin (Abcam: AB6276) diluted ...
9
A human T cell lymphoma secreting an immunoglobulin E specific helper factor
... An 8-yr-old nonallergic girl with non-Hodgkin's lymphoma had markedly elevated serum IgE at presentation (greater than 10,000 IU/ml), negative skin tests to a battery of 24 common allergens, and no evidence of parasitic ...
7
Tax 1-Independent Induction of Vascular Endothelial Growth Factor in Adult T-Cell Leukemia Caused by Human T-Cell Leukemia Virus Type 1
... Tax 1. In order to determine the effect of Tax 1 on the ability of Sp1 to bind its responsive elements, we performed EMSAs using a probe containing the Sp1 consensus ...Tax 1-transfected cells ...
7
MicroRNAs regulate T-cell production of interleukin-9 and identify hypoxia-inducible factor-2a as an important regulator of T helper 9 and regularoty T-cell differentiation
... helper T cell (Th) development and ...regulatory T cell (Treg) differentiation were capable of supressing IL-9 expression when they were overexpressed in Th9 ...
34
Astrocyte-specific expression of human T-cell lymphotropic virus type 1 (HTLV-1) Tax: induction of tumor necrosis factor alpha and susceptibility to lysis by CD8+ HTLV-1-specific cytotoxic T cells.
... Human T-cell lymphotropic virus type 1 (HTLV-1) was first isolated in 1980 (43) and is endemic in the southern regions of Japan, the Caribbean, the equatorial regions of Africa, South America ...
7
HTLV 1 bZIP factor supports proliferation of adult T cell leukemia cells through suppression of C/EBPα signaling
... with cell growth, but did not inhibit the C/EBPα- induced expression of anti-apoptotic genes including BCL2 and FLIP , suggesting that HBZ may fulfill its anti-apoptotic function through dysregulation of C/EBPα ...
13
Activation of the human T-cell leukemia virus type I enhancer is mediated by binding sites for Elf-1 and the pets factor.
... We have now shown that Elf-1 binds to PuBi and PuB2 in vitro and that Elf-1 binding or that of a closely related factor predominates in nuclear extracts from a human T-cell line Jurkat o[r] ...
7
Persistent human immunodeficiency virus type 1 infection in human fetal glial cells reactivated by T-cell factor(s) or by the cytokines tumor necrosis factor alpha and interleukin-1 beta.
... control of the productive and persistent course of HIV-1 in human fetal glial cells 29 and testing other known cytokines for their abilities to release persistence from astrocyte culture[r] ...
7
Human T-Cell Leukemia Virus Type 1 (HTLV-1) bZIP Factor Requires Cellular Transcription Factor JunD To Upregulate HTLV-1 Antisense Transcription from the 3′ Long Terminal Repeat
... the cell activation status (9, 21, ...both cell lines, Western blot analysis revealed that serum depletion de- creased c-Jun expression ...FIG 1 HBZ upregulates antisense transcription from 3= LTR in ...
9
1 alpha,25 dihydroxyvitamin D3 induces maturation of the human monocyte cell line U937, and, in association with a factor from human T lymphocytes, augments production of the monokine, mononuclear cell factor
... that 1 alpha,25(OH)2D3(10(-11)- 10(-10) M) induces maturational changes in the U937 cells similar to those produced by conditioned medium from lectin-stimulated T lymphocytes (increase in Fc receptors and ...
10
Neuroprotective coordination of cell mitophagy by the ATPase Inhibitory Factor 1
... in 1% bovine serum albumin in tris buffered saline (TBS-T) overnight (4 ...(1:2000; Cell Signaling; 60 min at room temperature (RT) in 5% non-fat milk) and an enhanced luminescence kit ...
44
Modulation of T-Cell Responses to a Recall Antigen in Human T-Cell Leukemia Virus Type 1-Infected Individuals
... medium with or without PPD (0.1 g/ml; Fuji Rebio) and phytohemagglutinin (PHA; 5 g/ml) in 96-well round-bottom tissue culture plates for 7 days. Culture supernatants were harvested prior to pulsing for subsequent ...
5
Human T-Cell Leukemia Virus Type 1 Tax Enhances Serum Response Factor DNA Binding and Alters Site Selection
... included 1 ⫻ EMSA buffer (20 mM HEPES, pH 7.4, 100 mM KCl, 0.5 mM EDTA, 1 mM dithiothreitol, ...glycerol), 1 g sheared salmon sperm DNA (Sigma), 0.02 g to 1 g SRF, 2 g Tax, and ...
10
Highly Enhanced Expression of CD70 on Human T-Lymphotropic Virus Type 1-Carrying T-Cell Lines and Adult T-Cell Leukemia Cells
... the cell surface were listed in ...necrosis factor (TNF) ligand superfamily member 7 (CD70), major histocompatibility com- plex class II (MHC II) DR  3 (HLA-DR3B), glucose trans- porter member 8 (GLUT8), ...
10
A novel HIV 1 restriction factor that is biologically distinct from APOBEC3 cytidine deaminases in a human T cell line CEM NKR
... derived cell clone #4, #5, and #7. To further address whether the HIV-1 resistance in the other non-permissive cell clones was due to an over- expression of any A3 cytidine deaminases, we measured ...
12
Induction of tumor necrosis factor alpha in human neuronal cells by extracellular human T-cell lymphotropic virus type 1 Tax.
... TNF- a in the CNS is associated with pathological events as well. Significantly, TNF- a is toxic for the myelin-producing cells of the CNS, the oligodendrocytes (36). Recently, Probert et al. (33) provided strong support ...
8
In vivo analysis of Nef’s role in HIV 1 replication, systemic T cell activation and CD4+ T cell loss
... Cohort 1 reconstitution. The host factor responsible for the reduction in viral load has not been identified, however, B42:01 is one of the HLA haplotypes for Cohort 1 and B42:01 has a negative ...
11
Molecular signatures of T cell inhibition in HIV 1 infection
... on T cells as a contributing factor for suboptimal T-cell responses in HIV infection both in vitro and in ...suppressor T cells in response to HIV ...
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