T. spiralis
Trichinella pseudospiralis vs T spiralis thymidylate synthase gene structure and T pseudospiralis thymidylate synthase retrogene sequence
... to T. spiralis muscle larvae being confined to a capsule, a possibility appears that the retrogene expression accounts for the life style adaptation ...
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Monoclonal antibody targeting complement C9 binding domain of Trichinella spiralis paramyosin impairs the viability of Trichinella infective larvae in the presence of complement
... of T. spiralis, this antibody also efficiently inhibits the binding of Ts-Pmy to human complement C9, suggest- ing that this antibody possesses the ability to functionally block the complement binding ...
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Preventive and therapeutic effects of Trichinella spiralis adult extracts on allergic inflammation in an experimental asthma mouse model
... from T. spiralis adult worms (Ts-AE) or muscle larvae (Ts-MLE) were used to treat the OVA-induced asthma before OVA sensitization (preventive) or during sen- sitization (therapeutic) in a mouse ...
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Cloning and expression of a Trichinella spiralis putative glutathione S transferase and its elicited protective immunity against challenge infections
... Trichinella spiralis muscle larvae (ML) are released in the stomach with the help of digestive enzymes, migrate to the intestine, and develop into the in- fective intestinal larvae (IIL) ...during T. ...
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Differentiation of Trichinella species (Trichinella spiralis/Trichinella britovi versus Trichinella pseudospiralis) using western blot
... of T. spiralis CWE reacting proteins able to distinguish between ...T. spiralis-, ...on T. spiralis and ...of T. spiralis and ...of T. spiralis CWE ...
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Molecular characterization of a cathepsin F like protease in Trichinella spiralis
... (amino acid 20–148) and a mature domain (amino acid 149–366). The conserved motifs in the cathepsin F cyst- eine protease family, ERFNAQ like LKFNAQ sequence, GNFN and GCNGG, were found in the pro-and mature domains. The ...
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Mapping of the complement C9 binding domain on Trichinella spiralis paramyosin
... helminthes, T. spiralis utilizes molecules or structures on the outermost cuticle/epicuticle to bind complement components, such as C1q, C3, C5, C8 and C9 [11-13], to evade the complement attach- ment by ...
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Molecular characterization of a Trichinella spiralis enolase and its interaction with the host’s plasminogen
... residues of proteins on pathogen surfaces to induce PLG to adopt a conformation that can be activated [7]. Hence, the lysine residues of receptors are essential for the ini- tial recruitment and subsequent conformational ...
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High prevalence of Trichinella pseudospiralis in Florida panthers (Puma concolor coryi)
... Trichinella spiralis is adapted to and most commonly occurs in domestic and wild pigs ...of T. spiralis in the US is rare ...Trichinella spiralis spills over into wildlife when there is a ...
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Molecular characterization of Trichinella spiralis aminopeptidase and its potential as a novel vaccine candidate antigen against trichinellosis in BALB/c mice
... of T. spiralis, sug- gesting that the TsAP is an indispensable protein and plays an important role in the life cycle of ...against T. spiralis infection, indi- cating TsAP might be a novel ...
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Sleep Deprivation Induces Changes in Immunity in Trichinella spiralis-Infected Rats
... cytotoxic T cells (CD3+CD8+) in spleen; while, in MLN, produces only an increase in natural killer cells ...total T cells (CD3+) in ...cytotoxic T cells (CD3+CD8+) and B cells ...in T helper ...
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Phenotypic Characterization of the Response to Infection with Trichinella spiralis in Genetically Defined Mouse Lines of the CBi IGE Stock
... T. spiralis was generously provided by Dr. Maria Dalla Fontana (Laboratorio de Zoonosis, Laboratorio Central de la Red Provincial de Laboratorios, Dirección de Bioquímica y Farmacia, Santa Fe, Argentina). ...
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Analysis of Diagnostic Efficiency of Excretory Secretory Antigens for Trichinella spiralis and Trichinella nativa in ELISA
... To work out ELISA parameters we have performed a comparative analysis of efficiency of two immune-en- zyme test systems based on T. spiralis and T. nativa antigens, respectively. The sensitivity of ...
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Mapping of the complement C1q binding site on Trichinella spiralis paramyosin
... several T-cell epitopes were predicted ...or T-cell epitopes induced a limited amount of protection in mice against ...T. spiralis infection [22, ...and T cell epitopes of TsPmy [24] or ...
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Intraperitoneal Inoculation: An Atypical Route of Trichinella spiralis Infection
... tissues. T. spiralis larvae observed in dif- ferent tissues of inoculated mice by compres- sion and/or immunostaining only 15 mpi, and then found in small intestinal epithelium at 12 h post ...
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Molecular identification and characterization of Trichinella spiralis proteasome subunit beta type 7
... vitro, T. spiralis infective larvae invade and migrate through the cell monolayer, leaving serpentine trails of dead and damaged cells ...with T. spiralis infective larvae, the mice immunized ...
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Species-Specific Antibody Responses to the Recombinant 53-Kilodalton Excretory and Secretory Proteins in Mice Infected with Trichinella spp.
... The 53-kDa glycoprotein is one of the E-S products and is highly concentrated in the E-S products, and so this protein may be a candidate immunodiagnostic antigen for trichinello- sis (25). However, in contrast to the ...
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Trichinella spiralis paramyosin activates mouse bone marrow derived dendritic cells and induces regulatory T cells
... host T cell regula- tory network, the rTsPmy was incubated with DCs and the rTsPmy-pulsed DCs were co-incubated with naïve mouse CD4 + T ...to T cells or TsPmy-pulsed DC- secreted Th1/Th2/Treg ...
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ANTHELMINTIC ACTIVITY OF GYNURA ANGULOSA DC. AGAINST TRICHINELLA SPIRALIS INFECTIONS IN MICE
... on T. spiralis in experimentally infected rats it is reported that 5 mg/kg body dose administration of oils for 2 consecutive weeks show more effectiveness in declining the number of adult worms and muscle ...
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Enhanced Immunological Memory Responses to Listeria monocytogenes in Rodents, as Measured by Delayed-Type Hypersensitivity (DTH), Adoptive Transfer of DTH, and Protective Immunity, following Lactobacillus casei Shirota Ingestion
... least 3 days prior to setting the L. monocytogenes-specific DTH assay, significantly enhances the DTH response at 24 and 48 h in the genetically different Wistar and Brown Norway rats as well as in BALB/c mice (Fig. 1, ...
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