T. thermophila
INDUCED RESISTANCE TO 6-METHYLPURINE AND CYCLOHEXIMIDE IN TETRAHYMENA. II. VARIATION WITHIN VEGETATIVE CULTURES OF MICRONUCLEATE T. THERMOPHILA AND AMICRONUCLEATE T. PYRIFORMIS
... Induction and selection of vegetative variants: Mutagenesis by N-methy1-N’- nitro-N-nitrosoguanidine (MNNG) , followed by vegetative growth subsequent to selection by 6MP o[r] ...
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INDUCED RESISTANCE TO 6-METHYLPURINE AND CYCLOHEXIMIDE IN TETRAHYMENA. I. GERM LINE MUTANTS OF T. THERMOPHILA
... The method used to select these mutations, the critical importance of backcrossing to wild type following mutagenesis, and the utility of these marker genes in furthe[r] ...
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α-Tubulin Mutations Alter Oryzalin Affinity and Microtubule Assembly Properties To Confer Dinitroaniline Resistance
... T. thermophila has abundant, dinitroaniline-sensitive tubu- ...ciliate T. thermophila is an excellent source of abundant dinitroaniline-sensitive tubulin, and we investi- gated it as a system ...
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ATP reception and chemosensory adaptation in Tetrahymena thermophila
... Chemosensory adaptation is the process by which sensory cells decrease their responsiveness to an external ligand during prolonged exposure to that stimulus. T. thermophila have already been reported to ...
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Entry and Killing of Tetrahymena thermophila by Bacterially Produced Shiga Toxin
... ABSTRACT Phage-encoded Shiga toxin (Stx) acts as a bacterial defense against the eukaryotic predator Tetrahymena thermophila. It is unknown how Stx enters Tetrahymena protozoa or how it kills them. Tetrahymena ...
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New Class of Cargo Protein in Tetrahymena thermophila Dense Core Secretory Granules
... several T. thermophila cDNA libraries for eight of these expressed se- quence tags (ESTs) by using a combination of a vector-specific and an EST-specific ...
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Accumulation of Spontaneous Mutations in the Ciliate Tetrahymena thermophila
... In an earlier MA study in T. thermophila, Brito et al. (2010) used the rate of clonal extinction as a measure of fitness effects of somatic mutations. They found that 1.25 MA lines per bottleneck went ...
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Biochemical and Genetic Evidence for the Presence of Multiple Phosphatidylinositol- and Phosphatidylinositol 4,5-Bisphosphate-Specific Phospholipases C in Tetrahymena
... Among the most important findings of this study were the identification and characterization of an expanded set of PI- PLC genes in Tetrahymena (Fig. 5 and 6). Our experiments suggested that PLC1, PLC2, and PRIP are ...
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DYF-1 Is Required for Assembly of the Axoneme in Tetrahymena thermophila
... Phenotypic studies. To measure the rate of phagocytosis, cells were fed with 0.2% India ink in SPP for 15 min at 30°C and fixed with 2% paraformaldehyde. FIG. 1. Tetrahymena has a DYF-1 ortholog. (A) A schematic ...
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RAD51 Is Required for Propagation of the Germinal Nucleus in Tetrahymena thermophila
... centrifugal elutriation. Daughter cells that had recently complement of DNA in the micronucleus is 4C during undergone cytokinesis were effectively size selected, cul- the amitotic division of the macronucleus and ...
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In Search of Telomeric Fusions in Telomere Mutants of Tetrahymena thermophila
... Tetrahymena thermophila (T. thermophila) has shown a severe anaphase arrest (Kirk et ...of T. thermophila TAS sequences were cut and ligated to mimic potential in vivo fusion ...
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Behavioral Effects of a Chemorepellent Receptor Knockout Mutation in Tetrahymena thermophila
... The only released factor that seems to be similar to our CSF is a compound called stomatin (31). Stomatin is found in the supernatant of Tetrahymena by using proce- dures that are very similar to our methods of obtaining ...
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Tudor Nuclease Genes and Programmed DNA Rearrangements in Tetrahymena thermophila
... In T. thermophila, the borders of DNA elimination of some deletion elements are very precise, while others are more vari- able, and how these borders are determined is not well under- stood ...
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Constitutive Secretion in Tetrahymena thermophila
... a T. thermophila database that was generated by combining the ...T. thermophila predicted proteome ...of T. thermophila gave rise to a higher percentage of false positives than ...
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The Tetrahymena thermophila Phagosome Proteome
... of T. thermophila that make it a strong model for the study of ...from T. thermophila and characterized their protein composition by multidimensional separation cou- pled with tandem mass ...
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C-5(6) Sterol Desaturase from Tetrahymena thermophila: Gene Identification and Knockout, Sequence Analysis, and Comparison to Other C-5(6) Sterol Desaturases
... conditions. T. thermophila strain CU428 (mpr1- 1/mpr1-1; mp-s, VII), designated the wild type (WT) in this work, and plasmid pBS-MnB-3 were a gift from ...
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Development of a genome-editing CRISPR/Cas9 system in thermophilic fungal Myceliophthora species and its application to hyper-cellulase production strain engineering
... The functional CRISPR/Cas9 system for gene editing has been successfully developed in yeasts and some fungi [28–40], including industrial filamentous fungi Aspergil- lus stains [33, 40], Trichoderma reesei [34] and ...
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Practical guidance for the implementation of the CRISPR genome editing tool in filamentous fungi
... recognizes T-rich PAM sequences and does not need a trans-acting crRNA (tracrRNA) due to the unique dual nuclease activity that cleaves not only the target DNA but also its own CRISPR-RNA (crRNA) ...
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CYTOGAMY: AN INDUCIBLE, ALTERNATE PATHWAY OF CONJUGATION IN TETRAHYMENA THERMOPHILA
... In cytogamy, the entire nuclear genetic information of an exconjugant is derived from a single meiotic product of its cytoplasmic parent.. Thus, an “instant” whole-genome homozyg[r] ...
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Phylogeny derived from homodimeric endonuclease correlates with its pre RNA substrates
... The phylogeny based on homodimeric endonuclease is new. Since the methanogens in euryarchaeal domain all have this enzyme, a finer characterization and classi- fication emerge. While the trees derived are all in rea- ...
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