TaqMan assay
Detection of clade types (clades I and II) within Anopheles funestus sensu stricto by the hydrolysis probe analysis (Taqman assay)
... Findings: A total of 53 An. funestus s.s. from Malawi and Mozambique were tested for detection of clade types using the hydrolysis probe analysis. Results were compared to DNA sequence analysis to verify the accuracy of ...
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Real-time PCR TaqMan assay for rapid screening of bloodstream infection
... PCR TaqMan assays, which target the bacterial 16S rRNA and fungal 18S rRNA, were evaluated with reference bacterial and fungal strains, clinical isolates, and direct blood cul- ture bottle ...PCR TaqMan ...
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A quantitative PCR (TaqMan) assay for pathogenic Leptospira spp
... immunosorbent assay (ELISA) and slide agglutination test (SAT), can detect different classes of antibody but may be subject to false positive reactions and require confirmation of these results by the ...
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First TaqMan Assay to Identify and Quantify the Cylindrospermopsin Producing Cyanobacterium Aphanizomenon ovalisporum in Water
... A number of studies have developed Real Time PCR approaches for the detection and quantification of some of the genes belonging to the hepatotoxin synthetase clusters (mcy, nda and cyr) using SYBER-Green I and ...
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Rapid Detection of West Nile Virus from Human Clinical Specimens, Field Collected Mosquitoes, and Avian Samples by a TaqMan Reverse Transcriptase PCR Assay
... virus TaqMan primers and probe were designed with the PrimerExpress software package (PE Applied Biosystems, Foster City, ...The TaqMan probes were labeled at the 5⬘ end with the FAM reporter dye and were ...
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Evaluation of a Multiplex Real Time Reverse Transcriptase PCR Assay for Detection and Differentiation of Influenza Viruses A and B during the 2001 2002 Influenza Season in Israel
... the TaqMan assay were compared to those of the regular one-step RT-PCR with gel detection with the same primer sets, SV-IFA, and virus isola- tion by TC with MDCK ...multiplex TaqMan assay ...
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The rs8099917 Polymorphism, When Determined by a Suitable Genotyping Method, Is a Better Predictor for Response to Pegylated Alpha Interferon/Ribavirin Therapy in Japanese Patients than Other Single Nucleotide Polymorphisms Associated with Interleukin 28B
... TaqMan assay. The rs8099917 polymorphism was determined by using TaqMan predesigned SNP genotyping assays, as recommended by the ...The TaqMan assay for determination of the genotype of ...
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The vector population monitoring tool (VPMT): high-throughput DNA-based diagnostics for the monitoring of mosquito vector populations
... A TaqMan assay was developed that detects all four malaria-causing Plasmodium species and discriminates ...The assay uses two probes, the first labelled with FAM detects ...the TaqMan ...
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Diagnostic assays and sampling protocols for the detection of Batrachochytrium dendrobatidis
... Chytridiomycosis is a major disease among amphib- ians and is responsible for population declines and extinctions (Daszak et al. 1999, 2000, Rachowicz et al. 2005). The causative agent is Batrachochytrium dendrobatidis, ...
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TaqMan allelic discrimination assay for A1 and A2 alleles of the bovine CSN2 gene
... the TaqMan system has been shown to be rapid, maximally sensitive and suitable for the detection of a range of ...of TaqMan assays for analysing SNP markers affecting specific performance parameters in ...
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Strain Specific Single Nucleotide Polymorphism Assays for the Bacillus anthracis Ames Strain
... of TaqMan MGB genotyping assays. The TaqMan MGB allelic discrimination assays were used to reli- ably detect and genotype samples containing as little as 100 fg of template DNA ...1. TaqMan endpoint ...
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Development of a Multitarget Real Time TaqMan PCR Assay for Enhanced Detection of Francisella tularensis in Complex Specimens
... real-time TaqMan PCR assay capable of rapidly and accurately detecting ...four TaqMan assays demonstrated that three (ISFtu2, 23kDa, and tul4) performed within our established criterion of a ...
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Rapid Identification of Yersinia enterocolitica in Blood by the 5′ Nuclease PCR Assay
... fluorogenic TaqMan assay has been recently described ...this assay seemed to have the potential for such ...a TaqMan PCR assay is described which is rapid and shows specific and ...
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Comparison of three PCR-based assays for SNP genotyping in plants
... design TaqMan, KASP and rhAmp ...respectively). TaqMan, KASP and rhAmp assays were performed in 5 μl using 384- well plates low Rox was used as a passive reference ...of TaqMan assay was ...
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A new TaqMan method for the reliable diagnosis of Ehrlichia spp in canine whole blood
... this TaqMan assay was well within tolerance (E = 97%, ...The TaqMan and PCRun® amplification time of detection was very reproducible at all titrations of genome tested, and both assays exhibited ...
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Comparison of the Xpert MTB/RIF Test with an IS6110 TaqMan Real Time PCR Assay for Direct Detection of Mycobacterium tuberculosis in Respiratory and Nonrespiratory Specimens
... IS6110- TaqMan assay may increase the release of mycobacterial DNA and reduce the activity of PCR ...IS6110-TaqMan assay to take more time than the Xpert MTB/RIF method, the IS6110- ...
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The Vector Population Monitoring Tool (VPMT): High-Throughput DNA-Based Diagnostics for the Monitoring of Mosquito Vector Populations
... A TaqMan assay was developed that detects all four malaria-causing Plasmodium species and discriminates ...The assay uses two probes, the first labelled with FAM detects ...the TaqMan ...
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Evaluation of Transported Dry and Wet Cervical Exfoliated Samples for Detection of Human Papillomavirus Infection
... We determined the feasibility of human papillomavirus (HPV) detection in cervical exfoliated cells collected as dry swab samples. Both dry cervical swab and specimen transport medium (STM) cervical swab samples were ...
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Detection of West Nile Virus Antigen in Mosquitoes and Avian Tissues by a Monoclonal Antibody Based Capture Enzyme Immunoassay
... with TaqMan Ct values of ⬍29 were positive by Ag capture, while all samples with Ct values of ⬎30 were negative in the Ag capture ELISA ...1). TaqMan Ct values are inversely related to the amount of viral ...
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Rapid Detection of Candida albicans in Clinical Blood Samples by Using a TaqMan Based PCR Assay
... commercialized TaqMan Minor Groove Binder (Ap- plied Biosystems), which enhances the melting temperature of the oligonucleotide, resulting in shorter probe ...
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