TaqMan probe
Molecular detection of human rhinoviruses in respiratory samples: a comparison of Taqman probe , SYBR green I and BOXTO based real time PCR assays
... a Taqman probe-based assay and constituted the best compromise amongst the techniques ...a probe-based de- tection with an intercalating agent-based detection should be renewed to identify a reliable ...
7
A TaqMan Probe Based Real Time PCR Assay for the Rapid Identification of the Emerging Multidrug Resistant Pathogen Candida auris on the BD Max System
... We developed an assay that is not only based on the TaqMan probe chemistry but also targets both the ITS1 and ITS2 of the ribosomal gene capable of rapidly detecting C. auris both from culture as well as ...
8
Download Download PDF
... the TaqMan® probe-based RT-PCR assay is more sensitive and more specific than histopathological ...the TaqMan® probe-based RT-PCR can improve diagnostic capacity by as much as 17% compared to ...
7
Evaluation of the SpeeDx Carba (beta) multiplex real time PCR assay for detection of NDM, KPC, OXA 48 like, IMP 4 like and VIM carbapenemase genes
... The sensitivity and specificity of the Carba (beta) assay for carbapenemase detection was assessed by testing an isolate panel of CPOs (n = 156) [10–14] and non-CPOs (n = 24) of various bacterial species, and comparing ...
7
Molecular Dynamics Simulations Towards The Understanding of the Cis Trans Isomerization of Proline As A Conformational Switch For The Regulation of Biological Processes
... The relationship between the bryozoan Bugula neritina and its symbiont "Candidatus Endobugula sertula" is a specific yet complex model for defensive symbiosis where the host larvae obtain chemical protection from ...
41
Comparative evaluation of published real time PCR assays for the detection of malaria following MIQE guidelines
... (either TaqMan probe or SYBR green) or background (QuantiFast probe Master Mix, QuantiFast SYBR green Master Mix or QuantiTect SYBR green Master ...
8
Practical Considerations in Design of Internal Amplification Controls for Diagnostic PCR Assays
... (TaqMan) probe is used in the inner region of the chimeric DNA to distinguish the PCR signal of the target and internal amplification control (IAC) in a real-time PCR assay for Salmonella ...
6
Detection of Toxoplasma gondii in Acute and Chronic Phases of Infection in Immunocompromised Patients and Pregnant Women with Real-time PCR Assay Using TaqMan Fluorescent Probe
... The TaqMan probe-based real-time PCR as- say was performed in this ...for Probe (Ampliqon, Denmark), ...of probe(10pmol/µl) labelled at the 5´ end with 6-carboxyfluorescein (FAM) and at the 3´ ...
9
Identification of Ciprofloxacin ResistantCampylobacter jejuni by Use of a Fluorogenic PCR Assay
... and TaqMan probes TAQ1 (5⬘ FAM-TTT GCT TCA GTA TAA CGC ATC GCA GC-TAMRA 3⬘), TAQ2 (5⬘ FAM-CCA CAT GGA GAT ACA GCA GTT TAT GAT G-TAMRA 3⬘), and TAQ3 (5⬘ TET-CCA CAT GGA GAT ATA GCA GTT TAT GAT GC-TAMRA ...Facility. ...
8
Detection of anatid herpesvirus 1 gC gene by TaqMan™ fluorescent quantitative real time PCR with specific primers and probe
... and TaqMan ™ probe (P) concentration selection criteria, which was performed according to 5 × 5 matrix of primers concen- trations ...and probe concentrations ...
10
Internally Controlled Triplex Quantitative PCR Assay for Human Polyomaviruses JC and BK
... primer-TaqMan probe set in a one-plex format on an ABI 7500 Fast instrument ...Man probe in the presence of each nonspecific amplicon ...BKPyV probe). None of the three TaqMan probes ...
8
Rapid Detection of Candida albicans in Clinical Blood Samples by Using a TaqMan Based PCR Assay
... the TaqMan technology combined with the GeneAmp 5700 real-time sequence detection system (Applied Biosystems) (6, ...the TaqMan principle generates a signal by cleaving a template- specific hybridization ...
6
A comparison of DNA sequencing and the hydrolysis probe analysis (TaqMan assay) for knockdown resistance (kdr) mutations in Anopheles gambiae from the Republic of the Congo
... hydrolysis probe analysis is the most sensitive and specific assay for detect- ing kdr mutations ...hydrolysis probe and sequencing assays due to a failed reaction of either the kdr-e or kdr-w ...
6
Development of a Multitarget Real Time TaqMan PCR Assay for Enhanced Detection of Francisella tularensis in Complex Specimens
... real-time TaqMan PCR assays were developed for combined use for the detection of ...develop TaqMan PCR assays, as opposed to other real-time assays, since TaqMan assays can provide high specificity ...
8
Close Geographic Association of Human Neoehrlichiosis and Tick Populations Carrying “Candidatus Neoehrlichia mikurensis” in Eastern Switzerland
... and TaqMan hydrolysis ...and TaqMan hydrolysis probes were chosen using PrimerExpress software, version ..._genus probe was modified with four locked nucleic acid (LNA) bases (Exiqon, Vedbaek, ...
8
Second Generation Cobas AmpliPrep/Cobas TaqMan HCV Quantitative Test for Viral Load Monitoring: a Novel Dual Probe Assay Design
... hydrolysis probe performed with the Cobas TaqMan thermal cycler to detect the target and a quantitative standard ...Cobas TaqMan HCV test for use with the High Pure System, ...
7
Real-time PCR TaqMan assay for rapid screening of bloodstream infection
... The use of the molecular diagnostic assay, real-time PCR TaqMan assay, is more effective for rapid screening of BSI than microbiological diagnosis. In this study, this technique allowed the simultaneous detection, ...
10
Comparison of the Xpert MTB/RIF Test with an IS6110 TaqMan Real Time PCR Assay for Direct Detection of Mycobacterium tuberculosis in Respiratory and Nonrespiratory Specimens
... As would be expected, both methods were highly specific and exhibited excellent sensitivity for smear-positive specimens (respiratory and nonrespiratory specimens) but a reduced sen- sitivity for smear-negative ...
5
Integrated Microfluidic Card with TaqMan Probes and High Resolution Melt Analysis To Detect Tuberculosis Drug Resistance Mutations across 10 Genes
... pncA probe specific to the His57Asp (CAC¡GAC) of Mycobacte- rium bovis and one probe for the common pncA silent mutation Ser65Ser ...rrs-A(1401)G-specific probe (detects the A-to-G change at position ...
9
Application of Digital PCR in the Analysis of Transgenic Soybean Plants
... TaqMan Assays TaqMan assays were modified from the original assays designed by the European Commission Joint Research Centre for these transgenic events using qPCR Please refer to: “Even[r] ...
15