targeted mutagenesis
Systematic analysis of the kalimantacin assembly line NRPS module using an adapted targeted mutagenesis approach
... geted mutagenesis of A domains appear to be even more challenging (Eppelmann et ...new targeted mutagenesis approach and a specificity- conferring code designed for use in ...
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Targeted Mutagenesis of Beta Lactoglobulin Gene in Caprine Fetal Fibroblasts by Context Dependent Assembly Zinc Finger Nucleases
... Targeted mutagenesis by zinc-finger nucleases (ZFNs) can be used to generate knock-out mamma- lian cell lines with high efficiency. A number of different methods have been developed for the de- sign and ...
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Efficient Targeted Mutagenesis in Medaka Using Custom-Designed Transcription Activator-Like Effector Nucleases
... for targeted genome editing. Here we demonstrate ef fi cient targeted mutagenesis in medaka ( Oryzias latipes ), which serves as an excellent vertebrate model for genetics and ...of targeted ...
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RNA-Seq analysis and targeted mutagenesis for improved free fatty acid production in an engineered cyanobacterium
... Genetic targets for improving FFA production in S. elongatus PCC 7942 include both general stress re- sponse genes as well as response mechanisms that may be specific for FFA-induced stress. Stress response genes that ...
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Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system
... Routine targeted mutagenesis opens up a new dimension in plant biology and should help to generate mutants in previously difficult to access genes, as well as simultaneously mutate multiple loci and ...
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RAPID SOUTHERN BLOT PROTOCOL FOR THE ANALYSIS OF TARGETED MUTAGENESIS IN THE MOUSE IGHG1 LOCUS
... The efficiency of homologous recombination relies on the lenght of the homology arms and the chromatin compactation of the locus. In ES cells the immuoglobulin locu is compacted. Thus, the homologous recombination events ...
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CRISPR/Cas-induced targeted mutagenesis with Agrobacterium mediated protein delivery
... In order to detect mutations, enrichment by restriction enzyme digestion was applied in the experiments described in the previous paragraph. In order to compare the efficiency of targeted mutagenesis after ...
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Highly efficient targeted mutagenesis in axolotl using Cas9 RNA guided nuclease
... Fig. 1. Efficient targeted mutagenesis of EGFP in Tg( CAGG:EGFP ) transgenic axolotls. (A) Distribution of phenotypes in axolotl (Ambystoma mexicanum) embryos from a mating between a wild-type and ...
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Highly Efficient Targeted Mutagenesis in Mice Using TALENs
... genome-wide targeted mutagenesis at high pre- ...introduce targeted mutations (Wefers et ...of targeted mutations it is instrumental to deliver DNA templates for HR together with TALEN mRNAs ...
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TALEN-mediated genome-editing approaches in the liverwort Marchantia polymorpha yield high efficiencies for targeted mutagenesis
... Results: Here, two different TALEN techniques, using custom and self-assembled TALEN constructs, were applied and compared. The MpNOP1 gene was selected as a candidate gene, as the respective knockout mutant has been ...
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Targeted mutagenesis of the human papillomavirus type 16 E2 transactivation domain reveals separable transcriptional activation and DNA replication functions.
... substitution mutagenesis of conserved amino acid residues in the N terminus has provided evidence that E2 is a multifunc- tional protein whose different activities can be ...
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Zinc finger nucleases for targeted mutagenesis and repair of the sickle-cell disease mutation: An in-silico study
... Background: Sickle cell disease (or simply, SCD) is an inherited hemoglobinopathy which is mostly prevalent among persons of African descent. SCD results from a monogenic (Hemoglobin, beta) point-mutation (substitution ...
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CRISPR/Cas9-targeted mutagenesis of Os8N3 in rice to confer resistance to Xanthomonas oryzae pv. oryzae
... allows targeted genome editing in organisms guided by a customizable small noncoding RNA called single guide RNA ...genes targeted by TAL effectors have been identified, the CRISPR/Cas9-mediated genome ...
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A New Mutant Class, Made by Targeted Mutagenesis, of Phage PRD1 Reveals That Protein P5 Connects the Receptor Binding Protein to the Vertex
... site-directed mutagenesis kit (Strat- agene) at the beginning of gene V (position 5305 in the PRD1 genome), resulting in plasmid ...vitro mutagenesis method was used to introduce an amber mutation into the ...
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Enhanced triacylglycerol production in the diatom Phaeodactylum tricornutum by inactivation of a Hotdog-fold thioesterase gene using TALEN-based targeted mutagenesis
... To examine the subcellular localization of ptTES1, we fused the full-length amino acids to eGFP. P. tricornutum transformants expressing the ptTES1-eGFP construct showed GFP fluorescence mainly in the plastid (Fig. 2), ...
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CRISPR/Cas9 mediated targeted mutagenesis of the fast growing cyanobacterium Synechococcus elongatus UTEX 2973
... The inability to produce a significant number of excon- jugants with constructs containing cas9 suggests that the gene is toxic in Synechococcus 2973 when it is introduced on a medium copy number plasmid. The fact that ...
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Targeted Mutagenesis of a Candidate t Complex Responder Gene in Mouse t Haplotypes Does Not Eliminate Transmission Ratio Distortion
... (R) Southern blots of DNA samples digested and hybridized with the indicated enzymes and probes are shown. Sizes of endogenow and targeted fragments are indicated in kb. Tc[r] ...
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Attenuation of Bunyamwera Orthobunyavirus Replication by Targeted Mutagenesis of Genomic Untranslated Regions and Creation of Viable Viruses with Minimal Genome Segments
... Previously, Lowen and Elliott 17 used a rational approach to reduce the lengths of BUNV S segment UTRs outwards from the coding region toward the termini and showed that the internal par[r] ...
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CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans
... C URRENT methods to generate mutations in the genome of Caenorhabditis elegans, including chemical mutagen- esis and imprecise excision of transposons, all rely on recovering mutations in large-scale mutagenesis ...
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Targeted Mutagenesis of Guinea Pig Cytomegalovirus Using CRISPR/Cas9-Mediated Gene Editing
... the mutagenesis of guinea pig cyto- megalovirus that utilizes clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9)-mediated genome editing to introduce ...
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