Targets for multiplex Real-Time PCR
Factors Influencing Multiplex Real-Time PCR
... of Multiplex PCR Careful consideration must be given to performing multiplex PCR, which may be the ideal approach for high-throughput, routine ...the time and cost associated with ...
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Multiplex Real Time PCR for Detection of Campylobacter, Salmonella, and Shigella
... the time frame in which treatment choices must be made is short and the conventional stool cultures lack good sen- sitivity, there is a need for a rapid, sensitive, and inexpensive de- tection ...as ...
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Comparison of a Multiplex Real Time PCR Assay with a Multiplex Luminex Assay for Influenza Virus Detection
... a real-time RT-PCR LDT to diagnose virally caused respiratory tract illnesses, includ- ing those caused by respiratory syncytial virus, metapneumovirus, influenza virus types A and B, parainfluenza ...
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Multiplex Real Time PCR for Detection of Anaplasma phagocytophilum and Borrelia burgdorferi
... the time of the year when ticks are actively seeking hosts, a group of several workers may collect upwards of 1,000 ticks per ...a real-time, multi- plex assay would be extremely beneficial for ...
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Rapid Identification of Mycobacterium tuberculosis and Nontuberculous Mycobacteria by Multiplex, Real Time PCR
... a multiplex, real-time PCR assay that can identify 93% of the pathogenic mycobacteria recovered in the Clinical Microbiology Laboratory of Stanford ...primer targets in the assay ...
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Multiplex Real Time PCR for Rapid Staphylococcal Cassette Chromosome mec Typing
... using multiplex real-time PCR with molecu- lar ...multiple targets to be distinguished in a single reaction. Real-time PCR assays also offer advantages in that they ...
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Multiplex real-time PCR assay for detection and differentiation of Bordetella pertussis and Bordetella parapertussis.
... of real-time ...tional PCR assay and multiplex real-time ...the targets in the real-time PCR, including the control human GAP- DH ...conventional ...
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Detection of diarrhoea-causing protozoa in general practice patients in The Netherlands by multiplex real-time PCR
... by time-consuming methods for the isolation of DNA from faecal specimens and the presence of inhibitory substances in such ...[9–11]. Real- time PCR reduces labour time, reagent costs ...
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Multiplex Real Time PCR Assay for Simultaneous Detection of Acanthamoeba spp , Balamuthia mandrillaris, and Naegleria fowleri
... published real-time PCR assay for detection of free-living amebas is a TaqMan assay specific for Acanthamoeba species causing keratitis ...the multiplex assay re- vealed that the former seems ...
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Use of Multiplex Real Time PCR To Diagnose Scrub Typhus
... novel multiplex real-time PCR assay to diagnose scrub ...This multiplex PCR assay includes two target ...this real-time PCR assay to a large number of ...
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Multiplex, Quantitative, Real Time PCR Assay for Cytomegalovirus and Human DNA
... a multiplex, quantitative, real-time PCR assay that amplifies cytomegalovirus (CMV) and human DNA in the same reaction tube, allowing for a viral load determination that is normalized to ...
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TaqMan Multiplex PCR Optimization For optimization of Multiplex PCR using 7500/7500 Fast, ViiA 7, and QuantStudio Real-Time PCR Systems
... If the required endogenous control target is available as a primer limited assay, you can begin validating your duplex PCR. However, if it is not, you must limit the primer concentration in the assay yourself. The ...
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Development of conventional and real time multiplex PCR assays for the detection of nosocomial pathogens
... Conventional multiplex PCR and SYBR Green based real time PCR assays were performed using genus and species specific ...Eubacterial PCR assay exhibited positive results for 46 ...
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Multiplex real–time RT–PCR for detecting chikungunya virus and dengue virus
... 2.5. Thermal profile and optimization conditions Separate reaction mixtures were carried out for each real- time RT-PCR with parameters adjusted for each virus target. The amplification reaction was ...
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Multiplex Real Time PCR Assay for Detection of Influenza and Human Respiratory Syncytial Viruses
... tiplex PCR assay and antigenic tests, conventional reverse transcription-PCR (RT-PCR) assays for detection of the glycoprotein G gene of HRSV (22) and the M2 gene of influenza A viruses (13) were ...
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Clinical Validation of Multiplex Real Time PCR Assays for Detection of Bacterial Meningitis Pathogens
... Singleplex real-time PCR assays have been developed to detect ...develop multiplex assays to reduce time and cost. New singleplex real-time PCR assays for serogroup ...
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Multiplex Real-Time PCR For The Detection Of Pathogenic Intestinal Parasites And Comparison With Parasitological Techniques
... For real-time multiplex PCR assay optimizations, primer limitation steps were performed in order to avoid any inhibition due to high abundant ...Two real-time multiplex ...
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Detection of intestinal protozoa in paediatric patients with gastrointestinal symptoms by multiplex real-time PCR
... by PCR and its clinical implications in paediatric patients are lacking, we chose to perform this study as a starting point for formulating further research questions and guiding future study ...of ...
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Real Time PCR and the icycler iq Real Time PCR Detection System for Quantitative PCR
... A serial dilution from 1x10 3 to 1x10 8 plasmids copies of IL1-b (ATCC#581768) and beta-Actin were assayed with hIL1-b Taqman PDAR kit (Perkin-Elmer) and hb-Actin PDAR (Perkin-Elmer).[r] ...
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Troubleshooting for PCR and multiplex PCR
... The first choice should be increasing the amount of primer for the "weak" loci at the same time with decreasing the amount of primer for all loci that can be amplified. The balance between these amounts is more ...
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