Terminal residues
Important roles of C terminal residues in degradation of capsid protein of classical swine fever virus
... exact residues responsible for the degradation of C ...C-terminal residues play more important roles in the degradation of C protein than N-terminal ...residues. Residues 260 to ...
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Feline Immunodeficiency Virus Vif N-Terminal Residues Selectively Counteract Feline APOBEC3s
... FIV Vif, similar to HIV-1 Vif, forms an E3 ubiquitin ligase complex, to induce feline A3 degradation (50). However, HIV-1 and SIV Vifs need the cofactor CBF-  to stabilize and form this complex (51, 52), whereas FIV and ...
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Dissipation Studies of Metribuzin in Alfisol Soils and its Terminal Residues in Potato Tubers
... The collected filtrate was transferred to separating funnel. Added 300 ml of water to it and extracted the solution thrice with 25 ml of dichloromethane. The dichloromethane fractions were pooled and passed through AR ...
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Differential Contributions of Tacaribe Arenavirus Nucleoprotein N-Terminal and C-Terminal Residues to Nucleocapsid Functional Activity
... Interestingly, while substitution of C-terminal residue P452, N453, D434, or D497 had no impact, mutations G472A/R473A, K474A, and D475A/L476A caused a severe inhibition on FLUC activity through an overall effect ...
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Functional Analysis of N-Terminal Residues of Ty1 Integrase
... In the intragenic complementation analysis, the mutants in- I28A and in-V45A demonstrated nearly normal levels of trans- position when coexpressed with WT IN. Although this result seems contradictory to the GST pulldown ...
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Two regions of the herpes simplex virus type 1 UL42 protein are required for its functional interaction with the viral DNA polymerase.
... Although a UL42 mutant RNA encoding a product lacking the 28 C-terminal residues resulted in wildtype levels of pol stimulation, the failure of large aminoterminal N-terminal and C-termi[r] ...
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Studies of the N-End Rule Pathway in Saccharomyces cerevisiae
... Figure 2. The N-end rule pathway in Saccharomyces cerevisiae. A: The Arg/N-end rule pathway. 44 See the main text for details. Yellow ovals denote the rest of a protein substrate. ‘‘Primary’’, ‘‘secondary’’ and ...
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Oligomerization of Hantavirus N Protein: C-Terminal α-Helices Interact To Form a Shared Hydrophobic Space
... N-terminal residues of hantavirus N proteins were predicted to form coiled coils (1), and (iii) oligomerization studies of N protein peptides corresponding to residues 3 to 75 assembled into trimeric ...
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The role of the N-terminus of the large subunit of ribulose-bisphosphate carboxylase investigated by construction and expression of chimaeric genes.
... coli, effectively replacing the 12 amino-terminal residues of the Anacystis large subunit with the 15 amino-terminal residues from wheat or maize (Fig. 3), the properties [r] ...
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“The N-End Rule Pathway: Its Physiological Importance and Role in Disease Pathology” by Subrata Kumar Pore, Rajkumar Banerjee, India.
... Dipeptides are used widely as N-end rule pathway inhibitors as a useful tools to study this pathway. As N- recognins have at least two types of well characterized binding sites, it had been shown that dipeptides (e.g. ...
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Roles of Carboxyl-Terminal and Farnesylated Residues in the Functions of the Large Hepatitis Delta Antigen
... these residues either directly contact HBsAg or are required to attain the proper structure that presents the HBsAg-interacting domain, then their conversion to alanine would inhibit HBsAg interaction even in the ...
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The C-Terminal Portion of the Hrs Protein Interacts with Tsg101 and Interferes with Human Immunodeficiency Virus Type 1 Gag Particle Production
... containing residues 1 to 502 (N-term), a C-terminal fragment containing residues 391 to 777 (C-term), full-length Hrs carrying the PSAP (resi- dues 348 to 351)-to-ASAA replacement (ASAA mutant), or ...
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Characterization of a Viral Phosphoprotein Binding Site on the Surface of the Respiratory Syncytial Nucleoprotein
... the residues selected are highly conserved among pneu- movirus N proteins ...these residues were replaced by Ala to generate 21 mutant ...critical residues that affected functions are part of PI ...
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Analysis of Mason-Pfizer Monkey Virus Gag Domains Required for Capsid Assembly in Bacteria: Role of the N-Terminal Proline Residue of CA in Directing Particle Shape
... N- and C-terminal deletions result in the assembly of cap- sids with thinner shells. A preliminary analysis of the electron micrographs of the capsids assembled from the different trun- cation mutants in the ...
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Three dimensional structure directs T-cell epitope dominance associated with allergy
... 5 residues 57–204, correlations were obtained with all four flexibil- ity criteria, and the correlations were ...10 residues C-termi- nal from flexible sites and 4–6 residues N-terminal from ...
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Molecular Characteristics of the Conserved Aspergillus nidulans Transcription Factor Mac1 and Its Functions in Response to Copper Starvation
... Functional conservation of low-Cu-responsive transcription factor Mac1 in selected fungal species. All selected Mac1 homologs contain an N-terminal Cu fist domain and C-terminal Cys-rich motifs, each ...
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Improving pentose fermentation by preventing ubiquitination of hexose transporters in Saccharomyces cerevisiae
... fermentation. The hexose transporter Hxt5 shows a moderate affinity for glucose affinity (10 ± 1 mM [30]), and this transporter is differently regulated as compared to Hxt1 and Hxt3 [36] which are low-affinity glucose ...
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Identification, Mutational Analysis, and Coactivator Requirements of Two Distinct Transcriptional Activation Domains of the Saccharomyces cerevisiae Hap4 Protein
... from residues 359 to 476 was the smallest fragment with transcriptional activity approximat- ing that of the entire Hap4 C terminus, we next tested whether the activity of this region depended on specific clusters ...
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Amino acid residues 201-205 in C-terminal acidic tail region plays a crucial role in antibacterial activity of HMGB1
... acid residues in C-terminal acidic tail region of HMGB1 (as shown in Figure ...acid residues 211-215, 206-215 and 186-200 respectively) could still exert antibacterial function on SA, JM109, ...
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Identification of Unusual E6 and E7 Proteins within Avian Papillomaviruses: Cellular Localization, Biophysical Characterization, and Phylogenetic Analysis
... Papillomaviruses (PVs) are a large family of small DNA viruses infecting mammals, reptiles, and birds. PV infection induces cell proliferation that may lead to the formation of orogenital or skin tumors. PV-induced cell ...
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