TGF-β1/Smad pathway
Exosomes derived from mesenchymal stem cells reverse EMT via TGF-β1/Smad pathway and promote repair of damaged endometrium
... anti- TGF-β1R (1:500; Cat# bs-23446R; Bioss Antibodies, USA), anti-smad2 (1:500; Cat# bs-0718R; Bioss Anti- bodies, USA), anti-p-smad2 (1:500; Cat# bs-3420R; Bioss Antibodies, USA), CK19 (1:500; Bioss, bs-2190), ...
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Original Article The protective effects of melatonin on peritoneal fibrosis induced by high glucose peritoneal dialysis in rats
... of TGF-β1, Col-I and α-SMA were significantly more increased in the model group than they were in the control ...peritoneal TGF-β1 and p-Smad 2/3 were significantly increased and that ...
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Original Article TGF-β1/Smad signaling, MMP-14, and MSC markers in arterial injury: discovery of the molecular basis of restenosis
... that TGF-β1 promotes the migration and recruitment of MSCs to the lesions to partici- pate in tissue repair and ...with TGF-β [18]. It has already been demonstrated that TGF-β1 can ...
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The effect of transforming growth factor-β1 on nasopharyngeal carcinoma cells: insensitive to cell growth but functional to TGF-β/Smad pathway
... then TGF-β1 was added (final concentration 10 ng/mL) for 3 ...for TGF-β type II receptor (TβR-II), Smad2, Smad3, Smad4, Smad7 and 28 cycles for ...
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Original Article Cross-talk between TGF-β/Smad pathway and Wnt/β-catenin pathway in pathological scar formation
... human TGF-β1 forward, 5’-CCC ACA ACG AAA TCT ATG ACA AG-3’, reverse, 5’-GCC ATG AGA AGC AGG AAA GG-3’; human β-actin forward, 5’-AGC GAG CAT CCC CCA AAG TT-3’, reverse, 5’-GGG CAC GAA GGC TCA TCA TT-3’; ...
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Original Article Effects of 18α-glycyrrhizin on TGF-β1/Smad signaling pathway in rats with carbon tetrachloride-induced liver fibrosis
... III, TGF-β1, p-Smad2, p-Smad3, Smad 7 and SP-1, in the liver, and the mRNA and protein expressions of these genes were determined in the liver by real time PCR and Western blot assay, ...of ...
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Original Article Effects of swertiamarin on TGF-β1/Smad signaling pathway in rats with carbon tetrachloride-induced liver fibrosis
... [9]. TGF-β1 has been identified as the most pro- fibrotic cytokine, and can elevate the expres- sion of collagen I in HSC, promote their tran- sition to a myofibroblast-like phenotype, and m- odulate key ...
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Herbal compound 861 prevents hepatic fibrosis by inhibiting the TGF β1/Smad/SnoN pathway in bile duct ligated rats
... alcohol gradient (100%, 95%, and 70%). Subsequently, antigens were retrieved with citrate buffer (0.01 M; pH 6) in a microwave oven. Following treatment with hydrogen peroxide at 37 °C for 30 min to block en- dogenous ...
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SHCBP1 promotes synovial sarcoma cell metastasis via targeting TGF-β1/Smad signaling pathway and is associated with poor prognosis
... the TGF- β /Smad signaling pathway. TGF- β 1 binds to TGF- β type I receptor, a transmembrane protein with Ser/Thr kinase domain, and then phosphorylates Smad2 and Smad3, which regu- ...
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Original Article Inhibition of the TGF-β/Smads signaling pathway attenuates pulmonary fibrosis and induces anti-proliferative effect on synovial fibroblasts in rheumatoid arthritis
... of inflammation factors, including IL-6 and TNF- α. The TGF-β1/Smad signaling pathway was activated during the onset of RA + PF. Infla- mmation in RA has been previously demon- strated, but ...
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TGF-β1 blockade of microglial chemotaxis toward Aβ aggregates involves SMAD signaling and down-regulation of CCL5
... of TGF-β signaling in mammalian cells ...of TGF-β1 in Aβ-injected rats ...on TGF-β1-suppressed microglia clustering and its concurrent effects on inhibiting SMAD2 phosphorylation and ...
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Original Article PDK4 regulates human cardiomyocyte viability, apoptosis, and inflammatory response via inhibiting TGF-β/Smad signaling pathway
... signaling pathway is one of the key signaling pathways in the pathogenesis of ...of TGF-β1, Smad2, and Smad3 [15]. Smad protein-dependent pathway is one of the classical signaling ...
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Rapamycin Inhibits Transforming Growth Factor β1 Induced Fibrogenesis in Primary Human Lung Fibroblasts
... mTOR/p70S6K pathway in a dose- dependent ...mTOR-Ser2448/p70S6K-Thr389 pathway in TGF-β1- induced ...of TGF-β1-induced collagen secretion is suggested as ...First, ...
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Original Article Knockdown of elF3a inhibits collagen synthesis in renal fibroblasts via Inhibition of transforming growth factor-β1/Smad signaling pathway
... them, Smad signaling is recognized as a major pathway of TGF-β1 signaling in progressive renal ...blocking TGF-β1 with neutralizing antibody, antisense oligonucle- otides, ...
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Original Article Triptolide inhibits lipopolysaccharide-induced proliferation, induces apoptosis and causes cell cycle arrest of human mesangial cells in vitro
... and TGF-β1 were significantly reduced following triptolide ...of TGF-β1, p-Smad2 and p-Smad3 proteins were significantly decreased in triptolide groups in a dose-dependent ...the ...
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Original Article Clearing heat and promoting diuresis method on TGF-β1/Smad signal pathway in airway remodeling of asthmatic rats
... that TGF-β (the cytokine promoting fibro- sis), which comes from various kinds of cells (such as macrophage, epithelial cell, fibroblast and eosinophil granulocyte), is the one of the main factors of structure ...
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Collaboration between Smads and a Hox protein in target gene repression
... mutations to the M1 site, the single mutation at position 808 reduced the binding of a Med fusion protein (GST-MedMH1) to M1 as compared with the wild-type sequence (Fig. 2B, lanes 1-4 and 5-8). The remaining three point ...
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synergistically increase PAI-1 expression in hepatic stellate cells of rats
... and TGF- β 1 could synergistically promote the production of type I collagen in HSCs as compared to treat- ment wth Ald alone, which however was blocked by ...
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Human cytomegalovirus promotes the activation of TGF-β1 in human umbilical vein endothelial cells by MMP-2 after endothelial mesenchymal transition
... Human cytomegalovirus TR virus was inoculated in the HELF and HUVECs in 24-well plates on coverslips according to MOI = 1 with or without recombinant ac- tive TGF-β1 (raTGF-β1, 15 ng/mL) treatment ...
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Icariside II ameliorates diabetic nephropathy in streptozotocin-induced diabetic rats
... (TGF-β/ Smad/CTGF) signaling, marker of proliferation Ki-67, and EdU+ LRCs in renal ...and TGF-β/Smad/CTGF signaling and increase the expression of von Willebrand factor, Ki-67, and EdU+ LRCs ...
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