The Annulus Fibrosus
Lamellar and fibre bundle mechanics of the annulus fibrosus in bovine intervertebral disc
... the annulus fibrosus, is a strong ring of fibrous tissue that retains an inner core, the nucleus pulposus, which is gel-like in young, healthy animals and ...The annulus is itself a composite ...
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Structural modelling of the annulus fibrosus - an anisotropic hyperelastic model approach at the lamellar level
... the Annulus Fibrosus, ...whole annulus deformation of the fibre angles, inter- lamellar behaviour, and incompressibility was analysed to assess their role in the disc ...
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Integrating Theoretical and Experimental Methods for Multi-Scale Tissue Engineering of the Annulus Fibrosus of the Intervertebral Disc
... the Annulus Fibrosus of the Intervertebral Disc Abstract There is a critical need for tissue engineered replacements for diseased and degenerated intervertebral discs in order to assuage low back pain while ...
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The Influence of Spinal Movements on the Lumbar Intradiscal Pressure and on the Tensile Stresses in the Annulus Fibrosus
... has been excluded from the present material. All the discs included here have either been normal o r showed only slight macroscopic changes in the nucleus pulposus. It has [r] ...
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Shape memory porous alginate scaffolds for regeneration of the annulus fibrosus Effect of TGF β3 supplementation and oxygen culture conditions
... engineering of the intervertebral disc with cultured annulus fibrosus cells using atelocollagen. 638[r] ...
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The Abnormal Annulus Fibrosus: Can We Infer the Acuteness of an Annular Injury?
... Abnormal Annulus Fibrosus: Can We Infer the Acuteness of an Annular Injury? Few issues in neuroimaging are as controversial as the nomenclature used to describe abnormalities of the lumbosacral ...the ...
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Morphological and biomechanical effects of annulus fibrosus injury and repair in an ovine cervical model.
... Defects of the intervertebral disc (IVD), which include injuries to the annulus fibrosus (AF) and herniation, are highly associated with dis- abling back and neck pain. 1,2 Extrusion of nucleus pulposus or ...
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Cell and Tissue Engineering for Annulus Fibrosus Repair: AO Foundation Collaborative Research Project
... Introduction Disk degeneration can lead to tearing of the annulus fibrosus (AF) and extrusion of the nucleus pulposus (NP). The current surgical repair strategies are suboptimal, and a tissue engineering ...
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Surgical removal and controlled trypsinization of the outer annulus fibrosus improves the bioactivity of the nucleus pulposus in a disc bioreactor culture
... pulposus in a disc bioreactor culture Pei Li 1 , Rongmao Shi 2 , Daosen Chen 3 , Yibo Gan 1 , Yuan Xu 4 , Lei Song 1 , Songtao Li 5 and Qiang Zhou 1* Abstract Background: The maintenance of nucleus pulposus (NP) ...
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Original Article Interleukin-1β induces metabolic and reactive oxygen species changes and apoptosis in annulus fibrosus cells
... 1 Department of Orthopaedics, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China; 2 Or- thopedic Institute, Soochow University, Suzhou, Jiangsu, China; 3 Changshu No.1 People’s Hospital, ...
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The Development of a Biomimetic Patch for Annulus Fibrosus Repair
... Potential early-stage intervention treatments for IVDD are being developed (i.e. NP arthroplasty); however, in order to effectively implement such a technique, the AF needs t[r] ...
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Autophagy in rat annulus fibrosus cells: evidence and possible implications
... AF cells treated with 3-MA, the autophagy inhibitor, showed a significant decrease in the autophagy incidence after a 24-hour stimulation of IL-1 b under serum deprivation, whereas an in[r] ...
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Histology staining on in vitro 3D poly(lactic-co-glycolic acid) seeded with annulus fibrosus, nucleus pulposus, and a combination of annulus fibrosus: nucleus pulposus (1:1) cells with and without fibrin scaffold
... copolymer has been widely applied in clinical settings as one of the suturing materials. It has been used as cartilage tissue engineering scaffolds because it is bioabsorbable and safe for human use. PLGA can be prepared ...
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FUNCTIONAL CELL PHENOTYPE INDUCTION WITH TGF-β1 AND COLLAGEN-POLYURETHANE SCAFFOLD FOR ANNULUS FIBROSUS
... Abstract Appropriate cell sources, bioactive factors and biomaterials for generation of functional and integrated annulus fibrosus (AF) tissue analogues are still an unmet need. In the present study, the AF ...
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Standardisation of basal medium for reproducible culture of human annulus fibrosus and nucleus pulposus cells
... from annulus fibrosus (AF) and nucleus pulposus (NP) of the intervertebral disc (IVD) in cell culture experiments utilising a broad variety of basal culture ...
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MMP 2 mediates local degradation and remodeling of collagen by annulus fibrosus cells of the intervertebral disc
... Quantification of gelatin films verified visual results (Figure 5e), with non- infected cells and shNon-transduced cells having similar percentages of decrease in staining (10.1% and 10.[r] ...
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Development of a two-step protocol for culture expansion of human annulus fibrosus cells with TGF-β1 and FGF-2
... Po-Hsin Chou 1,2 , Shih-Tien Wang 1,2 , Hsiao-Li Ma 1,2 , Chien-Lin Liu 1,2 , Ming-Chau Chang 1,2 and Oscar Kuang-Sheng Lee 3,4,5* Abstract Background: Different biologic approaches to treat disc regeneration, including ...
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A novel electrospun-aligned nanoyarn/three-dimensional porous nanofibrous hybrid scaffold for annulus fibrosus tissue engineering
... Dovepress Electrospun hybrid scaffold for annulus fibrosus tissue engineering the cellular phenotype. As specific phenotypes to distinguish AF cells are limited and there is no consensus regarding the ...
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Fluid-induced, shear stress-regulated extracellular matrix and matrix metalloproteinase genes expression on human annulus fibrosus cells
... This aim of the study was to investigate the expression of the ECM ( COLI , COLIII and aggrecan ) and matrix metalloproteinase ( MMP-1 , MMP-3 and ADAMTS-4 ) genes in hAF cells following[r] ...
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Effects of initial boost with TGF-beta 1 and grade of intervertebral disc degeneration on 3D culture of human annulus fibrosus cells
... of annulus tissue degeneration on the re-differentiation capacity of expanded AF cells in 3D polymer scaffolds, gene expression profiles were stratified by degeneration ...degenerated annulus ...
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