The fusion protein system
Development and Applications of a Calmodulin Based Fusion Protein System for the Expression and Purification of WW and Zinc Finger Modules
... ZF23 Fusion Protein in BL21 The ZF23 fusion protein was transformed into BL21 competent ...the protein of interest was eluted with 5 mM EDTA ...of protein as determined by ...
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Role of Sequence and Structure of the Hendra Fusion Protein Fusion Peptide in Membrane Fusion
... membrane fusion and disordering, mutations within the FP could affect potential FP-FP or FP-TMD ...isolated fusion peptides and transmembrane domains, suggesting that these two domains might associate later ...
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The cytoplasmic domain of the gamete membrane fusion protein HAP2 targets the protein to the fusion site in Chlamydomonas and regulates the fusion reaction
... gamete-specific protein in any system that is broadly conserved and shown by gene disruption to be essential for gamete ...to protein location and ...or fusion, although short deletions that ...
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Research Progress of Fusion Enzyme and the Effect of Peptide on Fusion Protein
... enzyme system, the fusion enzyme has the following three advantages ...the fusion enzyme can separate the two proteins and control the distance between them, so as to accelerate the catalytic ...
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A domain of murine retrovirus surface protein gp70 mediates cell fusion, as shown in a novel SC-1 cell fusion system.
... For MoMLV propagated in different cell lines, the virus genomes are alike, but components obtained from the host cell (i.e., membrane components or postranscriptional modifica- VOL.. The[r] ...
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Fusion tags for protein solubility, purification and immunogenicity in Escherichia coli: the novel Fh8 system
... recombinant protein production but, as a bacterial cell, it also has its issues: the aggregation of foreign proteins into insoluble inclusion bodies is perhaps the main limiting factor of the ...recombinant ...
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IMPROVEMENT OF hGM-CSF EXPRESSION USING A FUSION SYSTEM BY DIRECTING PROTEIN TO THE PERIPLASMIC SPACE
... expressed protein and the expressed protein was in insoluble ...of protein with maltose-binding protein in pMAL-c2 makes the protein soluble but the expression was low, however in case ...
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The Unique Transmembrane Hairpin of Flavivirus Fusion Protein E Is Essential for Membrane Fusion
... model system for flavivirus membrane ...the fusion process, but TM interactions apparently contribute to the stability of the postfusion E trimer and the completion of the merger of the ...
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A fusion peptide in the spike protein of MERS coronavirus
... putative fusion peptide in a biological system, a syncytium assay based on the expression of complete S protein was established, similar to that described for the avian coronavirus IBV ...spike ...
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Information fusion in the immune system
... information fusion in a particular man- ner. The cells of the immune system, through their different repertoires of receptors, sense different levels of information relat- ing to the state of the tissues of ...
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Regulation of Paramyxovirus Fusion Activation: the Hemagglutinin-Neuraminidase Protein Stabilizes the Fusion Protein in a Pretriggered State
... (HA)-neuraminidase protein (HN) of paramyxoviruses carries out three discrete activities, each of which affects the ability of HN to promote viral fusion and entry: receptor binding, receptor cleaving ...
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Cell-Cell Fusion Induced by the Avian Reovirus Membrane Fusion Protein Is Regulated by Protein Degradation
... p10 fusion-associated small transmembrane protein of avian reovirus induces extensive syncytium formation in transfected ...cell-cell fusion is restricted by rapid degradation of the majority of ...
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Membrane fusion of Semliki Forest virus involves homotrimers of the fusion protein.
... This in vitro fusion process exhibits the characteristics of the infectious fusion reaction of the virus with cells, in that it is strictly dependent on a mildly acidic pH and on the pre[r] ...
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An mRNA-protein Fusion at N-terminus for Evolutionary Protein Engineering
... nascent protein (phenotype) to its mRNA (genotype) covalently through the N-terminus was ...nascent protein via the phenylalanine derivative when the mRNA uses the amber suppressor tRNA to decode the amber ...
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Fusion Buffet System
... cart FUSION PKG1BK Above package w/all Black Glass w/out cart Get started easily with a Fusion Package which includes everything you need for hundreds of buffet ...your Fusion Buffet ...
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The Coronavirus Spike Protein Is a Class I Virus Fusion Protein: Structural and Functional Characterization of the Fusion Core Complex
... spike protein can be classified as a class I viral fusion ...The protein has, however, several unusual features that set it ...virus fusion proteins known so far is the cleavage of the ...
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A Single Amino Acid Change in the Newcastle Disease Virus Fusion Protein Alters the Requirement for HN Protein in Fusion
... F protein was explored by introducing single point mutations into the F gene ...the protein or the fusion activity of the ...mutant protein was cleaved and expressed at the cell surface ...
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Mutations in the fusion peptide and heptad repeat regions of the Newcastle disease virus fusion protein block fusion.
... In summary, nonconservative mutations along one side of a predicted ao helix in both the fusion peptide and the adjacent heptad repeat region of the NDV fusion protein block its fusion a[r] ...
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Functional Analysis of the Putative Fusion Domain of the Baculovirus Envelope Fusion Protein F
... putative fusion peptide, and this suggests that the F protein can properly function with a smaller hydrophobic ...putative fusion peptide of SeMNPV F are well defined, because the two con- served ...
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Localization of a Region in the Fusion Protein of Avian Metapneumovirus That Modulates Cell-Cell Fusion
... F protein, thus leading to a high fusion ...B-F protein, the cor- responding residues are H294, E295, R296, E297, K405, R435, and K438 ...A-F protein led to significantly decreased ...
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