The restriction enzyme analysis
RESTRICTION ENZYME ANALYSIS OF DNA
... RESTRICTION ENZYME ANALYSIS OF DNA Background Many of the revolutionary changes that have occurred in biology over the past fifteen years can be attributed directly to the ability to manipulate DNA ...
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Identification and characterization of Serpulina hyodysenteriae by restriction enzyme analysis and Southern blot analysis
... Chromosomal DNA restriction enzyme analysis and Southern blot hybridization were used to characterize Serpulina hyodysenteriae strains.. When chromosomal DNAs from selected strains (refe[r] ...
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Identification of various medically important Candida species in clinical specimens by PCR restriction enzyme analysis
... Istituto di Microbiologia, Universita` Cattolica Sacro Cuore, 1-00168 Rome, Italy Received 10 June 1996/Returned for modification 17 July 1996/Accepted 2 December 1996 A single primer pair amplifying a cytochrome P-450 ...
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Novel Allelic Variants of Mycobacteria Isolated in Brazil as Determined by PCR Restriction Enzyme Analysis of hsp65
... the restriction enzyme; that tolerance was different from the position tolerances of ...using restriction enzymes that generated DNA fragments in the 800- to 100-bp range that were separated on ...
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Detection and identification of mycobacteria in formalin fixed, paraffin embedded tissues by nested PCR and restriction enzyme analysis
... and restriction enzyme analysis of the PCR products was developed for the rapid detection and identification of Mycobacterium bovis and ...the restriction endonuclease Sau96-I allowed the ...
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PCR Restriction Enzyme Analysis for Detection ofCandida DNA in Blood from Febrile Patients with Hematological Malignancies
... Universita` Cattolica del Sacro Cuore, 1-00168 Rome, Italy Received 10 August 1998/Returned for modification 2 November 1998/Accepted 1 March 1999 Blood samples were drawn daily from 72 patients who had hematological ...
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Comparison of Restriction Enzyme Analysis, Arbitrarily Primed PCR, and Protein Profile Analysis Typing for Epidemiologic Investigation of an Ongoing Clostridium difficileOutbreak
... by restriction enzyme analysis (REA), arbitrarily primed PCR (AP-PCR), and protein profile analysis (PP) ...PP. Analysis of the data indicated the presence of a predominant strain among ...
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Comparison of polymerase chain reaction-restriction enzyme analysis method and DNA sequence analysis results in the identification of non-tuberculous mycobacteria
... reaction-restriction enzyme analysis (PRA) method and DNA sequence analysis method were utilized to target a gene region that codes the 65-kDa heat-shock protein for typing 150 suspected NTM ...
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Diagnosis of contagious caprine pleuropneumonia by detection and identification of Mycoplasma capricolum subsp capripneumoniae by PCR and restriction enzyme analysis
... by restriction enzyme cleavage for the identification of ...by restriction enzyme analysis of amplified DNA from mycoplasmas of the ...
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Differentiating Taenia solium andTaenia saginata Infections by Simple Hematoxylin Eosin Staining and PCR Restriction Enzyme Analysis
... with restriction enzyme analysis (PCR-REA), were tested on samples from 40 individuals infected with ...Distinct restriction patterns for ...
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Identification of Two Novel Mycobacterium avium Allelic Variants in Pig and Human Isolates from Brazil by PCR Restriction Enzyme Analysis
... Agropecua´ria, Centro Nacional de Pesquisa em Suı´nos e Aves, Conco´rdia-Santa Catarina, 3 Brazil Received 22 February 1999/Returned for modification 8 April 1999/Accepted 27 April 1999 Mycobacterium avium complex (MAC) ...
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Physical map of bacteriophage BF23 DNA: restriction enzyme analysis.
... in BalI digests of BF23st(+) DNA are listed as molecular weights without fragment numbers. Since EcoRI-1 is not[r] ...
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Comparison of epidemic and endemic group G streptococci by restriction enzyme analysis
... Restriction enzyme profiles of group G beta-hemolytic streptococci associated with a point source outbreak.. and an outbreak of sporadic pharyngitis in two different communities were com[r] ...
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Restriction enzyme analysis of partially transformation-defective mutants of acute leukemia virus MC29.
... The presence in the Q8 cell line of a provirus which has a ClaI site and the fact that the mutants lacked this implied that the deletions or. mutations could be found in this area[r] ...
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Comparison of Ribotyping and Restriction Enzyme Analysis for Inter and Intraspecies Discrimination of Bordetella avium and Bordetella hinzii
... REA results for B. avium suggest new variants may have recently emerged. HinfI profiles BA009 to BA014, which clus- ter together in one of the two major B. avium HinfI groups, are associated exclusively with isolates ...
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Restriction enzyme analysis of plasmid DNA and bacteriophage typing of paired Staphylococcus aureus blood culture isolates
... Table 3 outlines the phage types and REAP DNA profiles of the pairs of isolates considered nonidentical by group designation. The first pair had dif- ferent REAP DNA profiles, whereas al[r] ...
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Comparison of restriction enzyme analysis and pulsed field gradient gel electrophoresis as typing systems for Candida albicans
... To evaluate the sensi- tivity and specificity of REA further, in the present study we compared REA and CHEF techniques by using 35 clinical isolates from separate patients in a large ter[r] ...
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Restriction Enzyme Analysis of Thymidine Kinase Gene in Four Iranian Isolates of Herpes Simplex Virus Type 1
... Polymorphisms of thymidine kinase gene in herpes simplex virus type 1: Analysis of c1inical isolates from herpetic keratitis patients and laboratory strains. Acyclovi[r] ...
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Comparative analysis of genetic variability among Borrelia burgdorferi isolates from Europe and the United States by restriction enzyme analysis, gene restriction fragment length polymorphism, and pulsed field gel electrophoresis
... (A) Fingerprint patterns obtained through Southern blot hybridization of the 83-kDa antigen gene to the chromosomal EcoRI digests of the tick isolates from central and eastern parts of t[r] ...
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Three adenovirus type 8 genome types defined by restriction enzyme analysis: prototype stability in geographically separated populations
... Although Ad8 is still prevalent (5, 16, 36), Adl9 has been largely supplanted by Ad37, a new adenovirus species first isolated and characterized by Schapp et al. Hierholzer, Fourth Inter[r] ...
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