THP-1-derived macrophages
Stimulation with a monoclonal antibody (mAb4E4) of scavenger receptor mediated uptake of chemically modified low density lipoproteins by THP 1 derived macrophages enhances foam cell generation
... atherosclerotic lesions. It is directed against an epitope that is poorly exposed in delipidated and solubilized apolipoprotein B-100 from modified LDL. mAb4E4, as well as its F(ab')2 and Fab fragments, enhanced the ...
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<p>Radiotherapy Increases 12-LOX and CCL5 Levels in Esophageal Cancer Cells and Promotes Cancer Metastasis via THP-1-Derived Macrophages</p>
... RNA was isolated by RNA Fast 2000 (Fastagen, Shanghai, China). First strand cDNA synthesis was performed by a cDNA reverse transcription kit (All-in-One First Strand Synthesis Kit, Genecopeia, Rockville, MD, USA). Next, ...
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Tumor-associated macrophages modulate resistance to oxaliplatin via inducing autophagy in hepatocellular carcinoma
... with macrophages, the GFP-LC3 redistribution was ...with macrophages could activate autophagy in HCC ...with macrophages, increased oxaliplatin cytotoxicity was observed in ATG5 siRNA-transfected HCC ...
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Macrophage phenotype in response to ECM bioscaffolds
... behavior, THP-1 macrophages were chosen because of their widespread use in the immunology and regenerative medicine field ...the THP-1 derived macrophages have a ...
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Apoptosis of THP-1 macrophages induced by protoporphyrin IX-mediated sonodynamic therapy
... Background: Sonodynamic therapy (SDT) was developed as a localized ultrasound-activated cytotoxic therapy for cancer. The ability of SDT to destroy target tissues selectively is especially appealing for atherosclerotic ...
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IL-17 induces macrophages to M2-like phenotype via NF-κB
... (A) THP-1-derived macrophages were treated with IL-17 at 0, 10, 50 and 100 ng/mL for 48 ...il-17-treated ThP-1-derived ...
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The epithelial-to-mesenchymal transition induced by tumor-associated macrophages confers chemoresistance in peritoneally disseminated pancreatic cancer
... and THP-1-derived macrophages were co-cultured over a distance, and this resulted in the morphological change of pancreatic cancer cells to spindle shapes ...M2-polarized macrophages ...
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Effects of copper sulfate-oxidized or myeloperoxidase- modified LDL on lipid loading and programmed cell death in macrophages under hypoxia
... Notes: Macrophages were incubated for 72 hours with 100 mg/mL of low-density lipoproteins under hypoxia (1% oxygen) or normoxia (21% ...(A) THP-1-derived macrophages or (B) ...
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Dual and Triple Epithelial Coculture Model Systems with Donor-Derived Microbiota and THP-1 Macrophages To Mimic Host-Microbe Interactions in the Human Sinonasal Cavities
... of THP-1- derived macrophages did not affect the growth of these bacterial ...donor 1, whereas for donor 2 a mild decrease in IL-8 levels was noted after 72 ...donor 1 ...
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Rhinovirus Replication in Human Macrophages Induces NF-κB-Dependent Tumor Necrosis Factor Alpha Production
... activate macrophages, but rhinovirus replication in macrophages has not been ...human macrophages causes activation and nuclear translocation of NF- B, leading to TNF- ␣ ...Using macrophages ...
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III. Ion Channel Expression in PMA-differentiated Human THP-1 Macrophages
... found almost exclusively in differentiated cells. We ex- plored whether changes in mRNA abundance could ex- plain these changes in K + channel expression. The levels of mRNA associated with the IRK1 and Kv1.3 channels ...
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Effect of n-3 fatty acids on the expression of inflammatory genes in THP-1 macrophages
... unstimulated macrophages, depending on the metabolic pathway of interest, the n-3 FA having the most import- ant effect is ...on macrophages were studied without induced inflamma- ...mediators ...
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Eradication of intracellular Francisella tularensis in THP-1 human macrophages with a novel autophagy inducing agent
... was derived by structural modification of the cyclooxygenase- 2 (COX-2) inhibitor, celecoxib, but is devoid of COX-2- inhibitory activity ...of 1 - 5 μM ...human macrophages, THP-1 ...
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Mono-ethylhexyl phthalate stimulates prostaglandin secretion in human placental macrophages and THP-1 cells
... LPS derived from Salmonella typhi- murium from List Biological Laboratory (Campbell, CA); COX-1 and COX-2 monoclonal antibodies, and NS-398, from Cayman Chemical (Ann Arbor, MI); NONIDET P-40 Substitute ...
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Monocyte-derived factors including PLA2G7 induced by macrophage-nasopharyngeal carcinoma cell interaction promote tumor cell invasiveness
... Although macrophages are known to be the major cells secreting IL-1β in tumor microenvironment, tumor cells such as prostate, breast, and head & neck cancer cells are also able to produce IL-1β to potentiate ...
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Selective killing of HIV 1 positive macrophages and T cells by the Rev dependent lentivirus carrying anthrolysin O from Bacillus anthracis
... [43]. Macrophages were similarly infected with an M-tropic virus, HIV-1(AD8) ...as macrophages, we used a monocyte-like cell line, THP-1, as well as primary human monocyte- ...
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Gene expression profiling of macrophages: implications for an immunosuppressive effect of dissolucytotic gold ions
... human THP-1 cell line which shares many properties with human monocyte-derived macrophages [32] but does not resemble monocytes-macrophages isolated from human ...that ...
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Effect of cyclooxygenase inhibition on cholesterol efflux proteins and atheromatous foam cell transformation in THP 1 human macrophages: a possible mechanism for increased cardiovascular risk
... strictor, and smooth muscle mitogen [5,6]. Little is known of the impact of these drugs on the cholesterol transport system. COX enzymes catalyze the rate-limiting step in the prostanoid biosynthesis pathway, converting ...
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Enhanced uptake of multiple sclerosis-derived myelin by THP-1 macrophages and primary human microglia
... These results obtained with freshly isolated human microglia indicate that microglia are indeed very capable of contributing to myelin phagocytosis in MS, likely to- gether with infiltrating macrophages. Previous ...
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High resolution analysis of aberrant regions in autosomal chromosomes in human leukemia THP 1 cell line
... el (6)(p21),+8,+der(9)t (9;11)(p22;q23) t(9;11) (q12; q23),-10,der(11)t(9;11)(p22;q23),del (12)(p11), del (17)(p11),der (20)ins(20;1)(p12;p22p36) [13]/48, idem, -del(12)(p11) [7]. We confirmed almost all the reported ...
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